نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد گروه علوم باغبانی، دانشکدۀ کشاورزی، دانشگاه تبریز، تبریز، ایران

2 دانشیار گروه علوم باغبانی، دانشکدۀ کشاورزی، دانشگاه تبریز، تبریز، ایران

3 استادیار گروه علوم باغبانی، دانشکدۀ کشاورزی، دانشگاه تبریز، تبریز، ایران

چکیده

با توجه به اهمیت تولید و تکثیر گیاهان عاری از ویروس در سیب­زمینی تاکنون تعدادی از عوامل دخیل در تکثیر سیب­زمینی (تولید گیاهچه­ها و ریزغده­های درون­شیشه‌ای عاری از ویروس) بررسی شده است. مطابق نتایج دیگر تحقیقات، افزودن مخلوطی از اسیدهای آمینه، تأثیر مهمی در کشت بافت گیاهان دارد و رشد را در بسیاری از موارد افزایش می‌دهد. این آزمایش به­منظور بررسی اثر چهار اسیدآمینۀ گلوتامین، آرژنین، آسپاراژین، سیستئین و ترکیب دوتایی آنها بر ریزغده‌زایی سیب‌زمینی در قالب طرح کاملاً تصادفی با سه تکرار اجرا شد. جوانه­های جانبی حاصل از شاخساره­های درون­­شیشه­ای به‌عنوان ریزنمونه و تحت شرایط استریل روی محیط­ کشت MS کشت شدند. کشت­ها در تاریکی مداوم و دمای 2±17 درجۀ سانتی‌گراد در ژرمیناتور نگهداری شدند. طی ماه اول کشت، سرعت ریزغده‌زایی و پس از اتمام دو ماه صفاتی نظیر درصد ریزغده‌زایی، ریزغده‌های فاقد دورۀ خواب ومتوسط وزن ریزغده اندازه­گیری شد. نتایج تجزیۀ واریانس داده­ها نشان داد که همۀ صفات مورد بررسی به‌جز ریزغدۀ فاقد دورۀ خواب تحت تأثیر چهار اسیدآمینۀ مذکور معنادار بودند. در پژوهش حاضر، اسیدآمینۀ آسپاراژین در ترکیب با اسیدآمینۀ آرژنین تأثیر مثبتی در بهبود اکثر صفات مهم از جمله متوسط وزن ریزغده و سرعت ریزغده‌زایی داشت.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of some Amino acids on increasing of the microtuberization potato (cv. Agria)

نویسندگان [English]

  • Yalda Ghazi-Vakili 1
  • Ali Reza Motallebi-azar 2
  • Fariborz Zare Nahandi 3
  • Naser Mahna 3

1 M.Sc. Student, Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

2 Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

3 Assistant Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

چکیده [English]

Due to the importance of production and propagation of virus-free potato plants, some of the factors involved in the propagation of potato (plantlet production and virus-free in vitro microtuberization) has been studied. In most reports, adding a mixture of amino acids play an important role in plant tissue culture and development of the explants in many cases increase. This experiment was conducted in a completely randomized design with three replications to investigate the effects of glutamine, arginine, asparagine, cysteine and their combinations on microtuberization of potatoes. Lateral buds from in vitro shoots were cultured on MS medium under sterile conditions. Cultures were kept in constant darkness and temperatures of 17 ± 2°C in a germinator. During the first month of culture microtuberization rate and after two months other microtuberization traits such as microtubers weight was measured. Analysis of variance showed that all traits except microtuber without rest were significantly affected by four amino acids. In this study, arginine in combination with asparagine had a positive role in improving the most important characteristics such as weight and microtuberization rate.
 

کلیدواژه‌ها [English]

  • arginine
  • Asparagine
  • dormancy
  • Microtuberization rate
  • Weight of microtuber
 
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