نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه علوم باغبانی و زراعی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

2 استادیار، گروه علوم باغبانی و زراعی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران.

3 استادیار، گروه گیاهپزشکی، مرکز تحقیقات کشاورزی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

چکیده

میکوریزا یکی از رایج‌ترین میکروارگانیزم‌های همزیست با گیاهان محسوب می‌شود. به‌منظور ارزیابی سازگاری توده‏های مختلف بذری گیاه کاسنی و تلقیح با قارچ میکوریزا، آزمایشی به‌صورت فاکتوریل در قالب طرح بلوک‏های کامل تصادفی شامل دو توده بذری (اردبیل و فارس) و تیمار همزیستی با گونه­های قارچ میکوریزا (Glomus mosseae، Glomus intraradices و عدم کاربرد) در سه تکرار در گلخانه در سال 1398 اجرا شد. میزان ترکیبات فیتوشیمیایی (فنول، فلاونوئید و فعالیت آنتی‏اکسیدانی، کلروفیل a، b و کل)، وزن تر و خشک ریشه و طول آن، جذب عناصر معدنی در گیاه کاسنی اندازه‏گیری شد. نتایج نشان داد که توده بذری و کاربرد قارچ‏های میکوریزا تأثیر معنی‌داری بر صفات رویشی، مقدار تولید کلروفیل، فنول و فلاونوئید کل و فعالیت آنتی‏آکسیدانی گیاه داشت. بالاترین مقدار تولید فنل کل (77/3 میلی‏گرم بر گرم وزن تر)، فلاونوئید کل (21/1 میلی‏گرم بر گرم وزن تر)، فعالیت آنتی‏آکسیدانی (23/5 میلی‏گرم بر گرم وزن تر) و پارامترهای رشد رویشی در تیمار توده اردبیل و تحت کاربرد قارچ G. intraradices و کم‌ترین مقدار آن‏ها در توده فارس در شرایط عدم کاربرد قارچ مشاهده شد. نوع توده بذری باعت تغییر در میزان رشد و ترکیبات فیتوشیمیایی گیاه می‌شود. با کاربرد قارچ‌های میکوریزا پارامترهای رشدی بهبود یافتند. تأثیر کاربرد قارچ G. intraradices، کارآمدتر از همزیستی ناشی از قارچ G. mosseae تشخیص داده شد. براساس نتایج پژوهش حاضر و با توجه به سازگاری میکوریزا با محیط زیست، استفاده از قارچ‏های مذکور به‌منظور افزایش عملکرد انواع گیاهان و به‌ویژه گیاهان دارویی قابل توصیه است.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Some Mycorrhizal Fungi Species on Quantitative and Qualitative Properties of Two Landraces of Chicory in Greenhouse Conditions

نویسندگان [English]

  • Ali Yazdan panah gohari 1
  • Marzieh Ghanbari Jahromi 2
  • Vahid Zarrin nia 3

1 M.Sc. Student, Department of Horticultural Sciences and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran.

2 Assistant Professor, Department of Horticultural Sciences and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran.

3 Assistant Professor, Department of Plant Protection, Agricultural Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran.

چکیده [English]

Mycorrhiza is a common group of microorganisms that can establish symbiotic relations with plants. To evaluate symbiosis and compatibility between a collection of chicory seeds and mycorrhizal fungi, through inoculation, a factorial experiment is set up in a randomized complete block design. Two batches of chicory seed (Ardabil and Fars) are inoculated by two species of mycorrhizal fungi (Glomus mosseae and Glomus intraradices) with the control group of the seeds not being inoculated at all. Three replicates have been arranged in a greenhouse. The research aims at examining how the two species of mycorrhizal fungi affect several plant traits and the amounts of phytochemical compounds (i.e. phenol, flavonoids, antioxidant activity, chlorophyll a and b, total chlorophyll), fresh and dry weights of roots and root length. It also deals with chicory roots’ ability to absorb several elements. Results show that seed batch and mycorrhizal fungi have significant impacts on vegetative traits, chlorophyll content, phenol, total flavonoids, and antioxidant activity in plants. The highest amounts of total phenol production (3.77 mg/g fresh weight), total flavonoids (1.21 mg/g fresh weight), antioxidant activity (5.23 mg/g fresh weight), root growth and chlorophyll content are observed in the Ardabil batch when treated with G. intraradices. The lowest amounts of these values are recorded in the Fars batch where no mycorrhizal inoculation has been applied. The two batches of seeds grow into plants with different growth rates and phytochemical compositions. The growth parameters of plants improve in the case of mycorrhizal inoculation. The effects of G. intraradices are more efficient than those of G. mosseae in symbiosis with chicory plants. Based on the results of the present study and considering the compatibility of mycorrhiza with the environment, the use of these fungi can substantially increase the yield of various plants, especially medicinal plants.

کلیدواژه‌ها [English]

  • Antioxidant activity
  • Biofertilizers
  • Chycorium intibus
  • Phosphorus
  • total phenol
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