نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه علوم باغبانی و زراعی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

2 استادیار، گروه علوم باغبانی و زراعی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران.

3 استادیار، گروه گیاهپزشکی، مرکز تحقیقات کشاورزی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

چکیده

میکوریزا یکی از رایج‌ترین میکروارگانیزم‌های همزیست با گیاهان محسوب می‌شود. به‌منظور ارزیابی سازگاری توده‏های مختلف بذری گیاه کاسنی و تلقیح با قارچ میکوریزا، آزمایشی به‌صورت فاکتوریل در قالب طرح بلوک‏های کامل تصادفی شامل دو توده بذری (اردبیل و فارس) و تیمار همزیستی با گونه­های قارچ میکوریزا (Glomus mosseae، Glomus intraradices و عدم کاربرد) در سه تکرار در گلخانه در سال 1398 اجرا شد. میزان ترکیبات فیتوشیمیایی (فنول، فلاونوئید و فعالیت آنتی‏اکسیدانی، کلروفیل a، b و کل)، وزن تر و خشک ریشه و طول آن، جذب عناصر معدنی در گیاه کاسنی اندازه‏گیری شد. نتایج نشان داد که توده بذری و کاربرد قارچ‏های میکوریزا تأثیر معنی‌داری بر صفات رویشی، مقدار تولید کلروفیل، فنول و فلاونوئید کل و فعالیت آنتی‏آکسیدانی گیاه داشت. بالاترین مقدار تولید فنل کل (77/3 میلی‏گرم بر گرم وزن تر)، فلاونوئید کل (21/1 میلی‏گرم بر گرم وزن تر)، فعالیت آنتی‏آکسیدانی (23/5 میلی‏گرم بر گرم وزن تر) و پارامترهای رشد رویشی در تیمار توده اردبیل و تحت کاربرد قارچ G. intraradices و کم‌ترین مقدار آن‏ها در توده فارس در شرایط عدم کاربرد قارچ مشاهده شد. نوع توده بذری باعت تغییر در میزان رشد و ترکیبات فیتوشیمیایی گیاه می‌شود. با کاربرد قارچ‌های میکوریزا پارامترهای رشدی بهبود یافتند. تأثیر کاربرد قارچ G. intraradices، کارآمدتر از همزیستی ناشی از قارچ G. mosseae تشخیص داده شد. براساس نتایج پژوهش حاضر و با توجه به سازگاری میکوریزا با محیط زیست، استفاده از قارچ‏های مذکور به‌منظور افزایش عملکرد انواع گیاهان و به‌ویژه گیاهان دارویی قابل توصیه است.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Some Mycorrhizal Fungi Species on Quantitative and Qualitative Properties of Two Landraces of Chicory in Greenhouse Conditions

نویسندگان [English]

  • Ali Yazdan panah gohari 1
  • Marzieh Ghanbari Jahromi 2
  • Vahid Zarrin nia 3

1 M.Sc. Student, Department of Horticultural Sciences and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran.

2 Assistant Professor, Department of Horticultural Sciences and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran.

3 Assistant Professor, Department of Plant Protection, Agricultural Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran.

چکیده [English]

Mycorrhiza is a common group of microorganisms that can establish symbiotic relations with plants. To evaluate symbiosis and compatibility between a collection of chicory seeds and mycorrhizal fungi, through inoculation, a factorial experiment is set up in a randomized complete block design. Two batches of chicory seed (Ardabil and Fars) are inoculated by two species of mycorrhizal fungi (Glomus mosseae and Glomus intraradices) with the control group of the seeds not being inoculated at all. Three replicates have been arranged in a greenhouse. The research aims at examining how the two species of mycorrhizal fungi affect several plant traits and the amounts of phytochemical compounds (i.e. phenol, flavonoids, antioxidant activity, chlorophyll a and b, total chlorophyll), fresh and dry weights of roots and root length. It also deals with chicory roots’ ability to absorb several elements. Results show that seed batch and mycorrhizal fungi have significant impacts on vegetative traits, chlorophyll content, phenol, total flavonoids, and antioxidant activity in plants. The highest amounts of total phenol production (3.77 mg/g fresh weight), total flavonoids (1.21 mg/g fresh weight), antioxidant activity (5.23 mg/g fresh weight), root growth and chlorophyll content are observed in the Ardabil batch when treated with G. intraradices. The lowest amounts of these values are recorded in the Fars batch where no mycorrhizal inoculation has been applied. The two batches of seeds grow into plants with different growth rates and phytochemical compositions. The growth parameters of plants improve in the case of mycorrhizal inoculation. The effects of G. intraradices are more efficient than those of G. mosseae in symbiosis with chicory plants. Based on the results of the present study and considering the compatibility of mycorrhiza with the environment, the use of these fungi can substantially increase the yield of various plants, especially medicinal plants.

کلیدواژه‌ها [English]

  • Antioxidant activity
  • Biofertilizers
  • Chycorium intibus
  • Phosphorus
  • Total phenol
Ali Abadi-Farahani, H., & Valad Abadi, S. A. R. (2009). The role of arbuscular Mycorrhizal fungus on coriander (Coriandrum sativum L.) in drought stress conditions. Journal of Soil Science (Soil and Water Science), 24 (1), 69-80.
Ali Asghar zad, N. (1997). Soil Microbiology and Biochemistry (Translation 9), First Edition. University of Tabriz Press. (In Persian).
Al-Karaki, G. N. (2006). Nursery inoculation of tomato with arbuscular mycorrhizal fungi and subsequent performance under irrigation with saline water. Scientia Horticulturae, 109, 1-7.
Arnon, D. I. (1949). Copper Enzymes in Isolated Chloroplasts. Polyphenoloxidase in Beta Vulgaris. Plant Physiology, 24, 1-15.
Aslani, Z., Hassani, M., Rasooli Sadaghiyani, M., Sefidkon, S., & Barin, M. (2012). Effect of two fungi species of arbuscular mycorrhizal (Glomus mosseae and Glomus intraradices) on growth, chlorophyll contents and P concentration in Basil (Ocimum basilicum L.) under drought stress conditions. Iranian Medicinal and Aromatic Plants, 27(2), 471-486. (In Persian).
Bahadori, F., Sharifi Ashorabadi, A., Mirza, M., Matinizadeh, M., & Abdousi, V. (2015). The effects of plant growth promoting rhizobacteria and arbuscular mycorrhizal fungi on N, P and K uptake and yield of Thymus daenensis Clak Iranian Journal of Medicinal and Aromatic Plants, 31(3), 527-538. (In Persian).
Bais, H. P., & Ravishankar, G. A. (2001). Cichorium intybus L. cultivation, processing, utility, value addition and biotechnology, with an emphasis on current status and future prospects. Journal of Science Food Agriculture, 81, 467-484.
Baslam, M., & Goicoechea, N. (2012). Water deficit improved the capacity of arbuscular mycorrhizal fungi (AM FUNGI) for inducing the accumulation of antioxidant compounds in lettuce leaves. Mycorrhiza, 22, 347-359.
Bastami, A., & Majidian, M. (2016). Comparison between mycorrhizal fungi, phosphate biofertilizer and manure application on growth parameters and dry weight of coriander (Coriandrum sativum L.) Medicinal plant. Journal of Science and Technology of Greenhouse Culture, 7(2), 23-33. (In Persian).
Brundrett, M. C., & Tedersoo, L. (2018). Evolutionary history of mycorrhizal symbioses and global host plant diversity. New Phytologiest Trust, 220(4), 1108-1115.
Chang, Y. L., Kim, D. O., Lee, K. W., Lee, H. J., & Lee, C. Y. (2002). Vitamin C equivalent anti-oxidant capacity (VCEAC) of phenolic phytochemicals. Journal of Agricultural and food chemistry, 50(13), 3713-3717.
Copetta, A., Lingua, G., & Berta, G. (2006). Effects of three AM fungi on growth, distribution of glandular hairs, and essential oil production in Ocimum basilicum L. var. Genovese. Mycorrhiza, 16, 485-494.
Dorman, H. J. D., Peltoketo, A., Hiltunen, R., & Tikkanen, M. J. (2003). Characterisation of the antioxidant properties of de-odourised aqueos extracts from selected Lamiaceae herbs. Food Chemistry, 83, 255-262.
Fadaee, E., Parvizi Y., Gerdakane M., & Khan-ahmadi M. (2018). The Effects of Mycorhiza (Glomus mosseae and Glomus intraradiceae) and Phosphorus on Growth and Phytochemical Traits of Dracocephalum moldavica L. under Drought Stress. Journal of Medicinal Plants, 17(2), 117-130. (In Persian).
Fan, L., Dalpé, Y., Fang, Ch., Dubé, C., & Khanizadeh, Sh. (2011). Influence of arbuscular Mycorrhizale on biomass and root morphology of selected strawberry cultivars under salt stress. Botany, 89 (6), 397-403.
Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., & Basra, S. M. A. (2009). Plant drought stress: Effects, mechanisms and management. Agronomy for sustainable development, 29, 185-212.
Garg, N., & Chandel, S. (2011). Effect of mycorrhizal inoculation on growth, nitrogen fixation and nutrient tuptakein Cicer arietinum (L.) under salt stress. Turkish Journal of Agriculture, 4, 1-35.
Ghanbari Kashan, M., Mirzakhani, M., & Farid Hashemi, S. (2017). Effect of organic and N and P chemical fertilizers application on oil percentage and some of ecological traits of Carthamus tinctorious. Agricultural Science and Sustanble Production, 27(4), 203-2016.
Hamel, C. (2004). Impact of arbuscular mycorrhizal fungi on N and P cycling in the root zone. Canadian Journal of Soil Science, 84(4), 383-395.
Hristozkova, M., Gigova, L., Geneva Stancheva, I., Vasileva, I., Sichanova, M., & Mincheva, J. (2017). Mycorrhizal fungi and microalgae modulate antioxidant capacity of basil plants. Journal of Plant Protection Research, 57(4), 10pp.
Jeffries, P. (2001). Achievements in the past and autlook for the future of AMF. Research School of Biosciences, University. Of kent, Canterbury, kent CT27NJ, VK.
Kapoor, R., Giri, B., & Mukerji, K. G. (2004). Improved growth and essential oil yield and quality in (Foeniculum vulgare mill.) on mycorrhizal inoculation supplemented with P-fertilizer. Bio resource Technology, 93, 307-311.
Kartal, N., Sokmen, M., Tepe, B., Daferera, D., Polissiou, M., & Sokmen, A. (2007). Investigation of the antioxidant properties of Ferula orientalis L. using a suitable extraction procedure. Food Chemistry, 100(2), 584-589.
Kirch, H. H., Vera-Estrella, R., Golldack, D., Quigley, F., Michalowski, C. B., Barkl, B. J., & Bohnert, H. J. (2000). Expression of water channel proteins in Mesembryanthemum crystallinum. Plant Physiology, 123, 111-124.
Kohneh, A., Haghparast Tanha, M., & Ramazanpour, H. (2007). The effect of arbuscular mycorrhizal fungi and phosphorus on the amount of phosphorus uptake by tea seedlings in sterile soil. Journal of Agricultural Sciences, 38(1), 11-17. (In Persian).
Lee, J., & Scagel, C. F. (2010). Chicoric acid levels in commercial basil (Ocimum basilicum) and Echinacea purpurea products. Journal of functional foods, 2, 77-84.
Marcel, G. A., viemken, A., & Sanders, I. R. (2003). Different arbuscular mycorrhizal fungi alter coezistence and resorce distribution between coocurring olant. New Physiologist, 157, 569-578.
Mohammadi Sardoueiyeh, S., Boroomand, N., & Moghbeli, E. (2019). Effect of different mycorrhizal species inoculation on concentration of nutrient elements, yield per plant and antioxidant activity in Peppermint (Mentha piperita) under salt stress. Journal of Soil Management and Sustainable Production, 8(4), 127-142. (In Persian).
Mulabagal, V., Wang, H., Ngouajio, M., & Nair, M. G. (2009). Characterization and quantification of health beneficial anthocyanins in leaf chicory (Cichorium intybus) varieties. European Food Research and Technology, 230, 47-53.
Muthusamy, V. S., Anand, S., Sangeetha, K. N., Sujatha, S., Arun, B., & Lakshami, B. S. (2008). Tannins present in Cichorium intybus enhance glucose uptake and inhibit adipogenesis in 3T3-L1 adipocytes through PTP1B inhibition. Chemico Biological Interacttions Journal, 174(1), 69-78.
Nadiyan, H. (2011). Effect of drought stress and Mycorrhizal symbiosis on phosphorus growth and absorption by two different sorghum cultivars in root morphology. Journal of Agricultural Science and Technology, Water and Soil Science, 15(57), 127-140.
Rahimi, A., Dovlati, B., Amirnia, R., & Heydarzade, S. (2020). Effect of application of mycorrhizal fungus and Azotobacter on physiological characteristics of Trigonella foenum-graecum L. under water stress conditions. Iranian Journal of Plant Biology, 11(4). (In Persian).
Ratti, N., Kumar, S., Verma, H. N., & Gautams, S. P. (2001). Improvement in bioavailability of tricalcium phosphate to Cymbopogon martini var. motia by Rhizobacteria, AMF and Azospirillum inoculation. Microbiology Research, 156, 145-149.
Riter Netto, A. F., Freitas, M. S., Martins, M. A., Carvalho, A. J., & Vitorazi Filho, J. Á. (2014). Efeito de fungos micorrízicos arbusculares na bioprodução de fenóis totais e no crescimento de Passiflora alata Curtis. Revista Brasileira de Plantas Medicinais, 16, 1-9.
Rozpadek, P., Wezowicz, K., Stojakowska, A., Malarz, J., Surowka, E., Sobczyk, P., Anielska, R., Wazny, R., Miszalski, Z., & Turnau, K. (2014). Mycorrhizal fungi modulate phytochimical production and antioxcidant activity of Chichoriyum intibus L. (Astraceae) under metal toxicity. Chemosphere journal, 112, 217-224.
Saleh Rastin, N. (1998). Biological fertilizers. Journal of soil and water sciences, 12(3), 1-36. (In Persian).
Sharma, A. K. (2003). Biofertilizer for sustainable agriculture. Agrobios (India). 407.
Sheng, M., Tang, M., Zhang, F. F., & Huang, Y. H. (2011). Influence of arbuscular mycorrhiza on organic solutes in maize leaves under salt stress. Mycorrhiza, 21, 423-430.
Zhang, R. Q., Zhu, H. H., Zhao, H. Q., & Yao, Q. (2013). Arbuscular mycorrhizal fungal inoculation increases phenolic synthesis in clover roots via hydrogen peroxide, salicylic acid and nitric oxide signaling pathways. Journal of Plant Physiology, 170(1), 74-79.
Zimare, S. B., Borde, M. Y., Jite, P. K., & Malpathak, N. P. (2013). Effect of AM Fungi (Gf, Gm) on Biomass and Gymnemic Acid Content of Gymnema Sylvestre (Retz.). The Proceedings of the National Academy of Sciences, India, Section B. Biological Sciences, 83, 439-45.
Zlatev, Z., & Lidon, F. C. (2012). An overview on drought induced changes in plant growth, water relations and photosynthesis. Emirates Journal of Food and Agriculture, 24, 57-72.3.