نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته کارشناسی ارشد، گروه زراعت، دانشکده کشاورزی، دانشگاه صنعتی شاهرود، شاهرود، سمنان، ایران.

2 دانشیار، گروه زراعت، دانشکده کشاورزی، دانشگاه صنعتی شاهرود، شاهرود، سمنان، ایران.

چکیده

به‌منظور بررسی محلول­پاشی عصاره مخمر بر خصوصیات فیزیولوژیکی و بیوشیمیایی لوبیا چشم­بلبلی(Vigna sinensis L.)  در شرایط تنش خشکی،آزمایشی به‌صورت فاکتوریل در قالب طرح بلوک­های کامل تصادفی با سه تکرار در سال زراعی 1397 در دانشکده کشاورزی دانشگاه صنعتی شاهرود (واقع در بسطام)، تحت تیمارهای تنش خشکی و محلول­پاشی عصاره مخمر به اجرا درآمد. تنش خشکی به‌‌صورت قطع آبیاری در دو مرحله 50 درصد گل­دهی و 50 درصد غلاف­بندی و تیمار بدون قطع آبیاری به‌عنوان تیمار شاهد بود. محلول‌پاشی عصاره مخمر با غلظت‌های صفر، دو، چهار و شش گرم بر لیتر انجام گرفت. نتایج این پژوهش نشان داد که با مصرف مخمر با غلظت‌های دو، چهار و شش گرم بر لیتر، میزان محتوای نسبی آب برگ 82/5 درصدی افزایش یافت. محتوای پرولین (74/30 درصد)، فلاونوئید برگ (83 درصد)، محتوای نسبی آب برگ (82/ 5 درصد)،  نیتروژن (82/3 درصد)، فسفر(22/22 درصد) و پتاسیم دانه (43 درصد) ازجمله صفاتی بودند که با کاربرد عصاره مخمر نسبت به تیمار شاهد افزایش یافتند. استفاده از غلظت شش گرم بر لیتر عصاره مخمر موجب افزایش عملکرد دانه (02/1 تن در هکتار)، آنتوسیانین برگ (072/0 میلی­گرم بر گرم وزن‌تر)، پرولین شد. نتایج نشان داد که غلظت شش گرم بر لیتر عصاره مخمر می‌تواند در کاهش شدت تنش خشکی مفید باشد. عصاره مخمر می‌تواند به‌عنوان یک راه‌کار اکولوژیکی، باعث افزایش پاسخ دفاعی در برابر تنش خشکی شود.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Yeast Extract on Physiological and Biochemical Indicators of Cowpea in Drought ‎Stress Conditions

نویسندگان [English]

  • sahar keramati 1
  • Ahmad Gholami 2
  • Mehdi Baradaran 2
  • Hamid Abbasdokht 2

1 Former M.Sc. Student, Department of Agronomy, Faculty of Agricultural, Shahrood University of Technology, Shahrood, Semnan, Iran.

2 Associate Professor, Department of Agronomy, Faculty of Agricultural, Shahrood University of Technology, Shahrood, Semnan, Iran.

چکیده [English]

In order to investigate the role of foliar application of yeast extract on physiological and Biochemical properties of cowpea (Vigna sinensis L.) under drought stress conditions, a factorial experiment has been conducted as a randomized complete block design with three replications in Agriculture Campus of Shahrood University of Technology (located in Bastam) during 2017 under drought stress and foliar application of yeast extract. Drought stress involves two stages of 50 present flowering and 50 present pod formation with control treatment (without irrigation interruption). Foliar application of yeast extract (two, four, six g/L, and control) are repeated twice with a seven-day interval, with the first one being 30 days after planting. Results show that drought stress has decreased relative water content of leaves. In contrast, relative water content (5.82%), proline content (30.74%), leaf flavonoid (83%), nitrogen (3.82%), phosphorus (22.22%), and potassium (43%) are among the traits that have increased with the use of yeast extract, compared to the control. Six g / L of the yeast extract has increased grain yield (1.02 t ha-1), leaf anthocyanin (0.072 mg /g fresh weight), and proline (31.62 mg /g fresh weight). It can be said that a concentration of six g/L of yeast extract can be useful to reduce the severity of drought stress. The yeast extract can act as an ecological strategy to improve the defensive response against environmental stresses.

کلیدواژه‌ها [English]

  • bio stimulant
  • Flavonoids
  • Grain yield
  • Phosphorus Grain
  • proline
Abbasi Siahjani, E. (2008). Effect of drought stress on morphological and agronomical characteristics of sunflower. M.Sc. thesis of Agronomy. Islamic Azad University of Tabriz. Iran. )In Persian(.
Abd El-Latif, E. S. M. (2006). Effect of Chemical, Organic Fertilizers and Spraying with Active Dry Yeast on Growth, Oil Production and Plant Constituents of Sage (Salvia officinalis L.) Plant. M. Sc. Thesis. Cairo University., Egypt, jornal of productivity and development. 155p.
Ahmad, F.E., & Suliman, A.S.H. (2010). Effect of water stress applied at different stages of growth on seed yield and water-use efficiency of Cowpea. Agriculture and Biology Journal of North America, 1(4), 534-540.
Alaei, Y., Khanghah, A.M., Jafari, M., & Khaneghah, A. (2012). Evaluation on leaf proline amount in three bread wheat cultivars in presence of two fertilizers containing amino acids in drought stress. World Applied Sciences Journal, 18(9), 1190-1192.
Amer, S. S. A. (2004). Growth, green pods yield and seeds yield of common bean (Phaseolus vulgaris L.) as affected by active dry yeast, salicylic acid and their interaction. Journal of Agricultural Science Mansoura University, 29 (3), 1407-1422.
Baque, M.A., Karim, M.A., Hamid, A., & Tetsushi, H. (2006). Effect of fertilizer potassium on growth, yield and nutrient uptake of wheat (Triticum aestivum L.) under water stress conditions. South Pacific Studes, 27(1), 25-35.
Barnett, J.A., Payne, R.W., & Yarrow, D. (1990). Yeast: Characteristics and Identification second ed. Press, Cambridge University., London, UK.1012 p.
Bates, L.S., Waldren, K.P., & Teare, L.D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil journal.  39, 205-207.
Chapman, H.D., & Pratt, P.F. (1982). Methods of Plant Analysis. I: Methods of Analysis for Soil, Plants and Water. Chapman Publishers, Riverside, University of California, Division of Agricultural Sciences, CA. 170 p.
El-Desouky, S.A., Wans, A. L., & Khedr, Z. M. (1998). Utlization of some natural plant extracts (of garlic and yeast) as seed – soaked materials to squash (Cucurbatia pepo L.). I- Effect on growth, sex expression and fruit yield and quality. Agricultural Science. Moshtohor, Zagazig. Univ., 35(2), 839-854.
El-Gamal, S. M. A. (2005). Physiological Response of Sweet Basil Plants Grown Under Stress Conditions as Affected by Yeast Extract, Ascorbic Acid and Potassium. Minufiy. Middle East Journal of Agriculture Research, 30(1), 25-50.
El-Garhy, A.M. (2002). Physiological Studies on Tolerance of Some Varieties of Faba Bean Plants Under Least Water Requirements. Ph. D. thesis, Agric., Botany Department., Faculty of Agriculyure., Minufiya Univ., Shebin El-Kom, Egypt, pp. 44-120.
El-Tohamy, W. A., El-Abagy, H. M., & El-Greadly, N. H. M. (2008). Studies on the effect of putrescine, yeast and vitamin C on growth, yield and physiological responses of eggplant (Solanum melongena L.) under sandy soil conditions. Australian Journal of Basic and Applied Sciences, 2(2), 296-300.
Emam, Y., & Seghatoleslami, M. J. (2005). Crop Yield:  Physiology and Processes. Shiraz University Press, Shiraz, Iran. 593 p. (In Persian).
Ezz El-Din, A. A., & Hendawy, S. F. (2010). Effect of dry yeast and compost tea on growth and oil content of Borago officinalis plant. Research Journal of Agriculture and Biological Sciences, 6(4), 424-430.
Fawzy, Z. F., Li, Y., Ouyang, Z., & Hoda, A. M. (2012). Influence of foliar application by EM “Effective microorganisms”, amino acids and yeast on growth, yield and quality of two cultivars of onion plants under newly reclaimed soil. Journal of Agricultural Science, 4(11), 26.
Flowers, T.J., Troke, P.F., Yeo, A.R. (1977). The mechanism of salt tolerance in halophytes. Annual Review of Plant Physiology. 28, 89–121.
Galavi, M., Hossein Abadi, A. A., & Heidari, M. (2006). Study of the effects of foliar application of iron, zinc and manganese on the qualitative and quantitative characteristics of wheat Hamoon in Sistan area. New agricultural Findings, 1(2), 104-110.
Garratt, L.C., Janagoudr, B.S., Lowe, K.C.,Anthony, P., Power, J.B., & Davey, M.R. (2002). Salinity tolerance and antioxidant status in cotton cultures. Free Radical Biology and Medicine, 33(4), 502-511.
Gonzales, P.R., & Salas, M.L. (1995). Improvement of the growth, grain yield, and nitrogen, phosphorus and potassium nutrition of grain corn through weed control. Journal of Plant Nutrition, 18, 3313-3324.
Haidar Al-madhagi, I.A.H. (2019). Effect of humic acid and yeast on the yield of greenhouse cucumber. Journal of Horticulture and Postharvest Research, 2(1), 67-82.
Hammad, S.A.R. (2008). Physiological and anatomical studies on drought tolerance of pea plants by application of some natural extracts. Annals of Agricultural Sciences .Ain Shams Univ., Cairo., 53(2), 285-305.
Hammad, S. A., & Ali, O. A. (2014). Physiological and biochemical studies on drought tolerance of wheat plants by application of amino acids and yeast extract. Annals of Agricultural Sciences, 59(1), 133-145.
Hemantaranja, A., & Gray, O. K. (1988). Iron and zinc fertilization with reference to the grain quality of Triticum eastivum L.. Journal of Plant Nutrition, 11, 1439-1450.
Hussain K., Nawaz, K., Majeed A., Khan F., Lin F., Ghani A., Raza G., Afghan S., Zia-ul-Hussnain S., & Ali K. (2010). Alleviation of salinity effects by exogenous applications of salicylic acid in pearl millet (Pennisetum glaucum L.) R. Br.) seedlings. African Journal of Biotechnology, 9, 8602-8607.
Hussein, M.M., Shaaban, M.M., & El-Saady, A.K.M. (2008). Response of cowpea plants grown under salinity stress to pk foliar applications. American Journal of Plant Physiology, 3(2), 81-88.
Jin, J., Wang, G., Liu, X., Pan, X., Herbert, S.J., & Tang, C. (2006). Interaction Between Phosphorus Nutrition and Drought on Grain Yield, and Assimilation of Phosphorus and Nitrogen in Two Soybean Cultivars Differing in Protein Concentration in Grains.  Journal of Plant Nutrition, 29, 1433- 1499.
Kafi, M., Barzoui, A., Salehi, M., Kamandi, A.S., Masoumi, A.S., & Vegetati, H. (2009). Physiology of Environmental Stresses in Plants. Mashhad University of Medical Sciences Publications, First edition, Mashahd, Iran. 502 p.
Karamlachab, A., Bakhshandeh, A. M., Qarineh, M. H., Fathi, Gh., & Moradi Telavat, M. R. (2014). Effect of Silicon Application on Morphophysiological Traits, Yield and Content of Wheat Grain Minerals Under drought stress conditions. Journal of Crop Production and Processing, 4(14), 133-144.
Kochaki, A. R., & Khaje Hosseini, M. J. (2008). Modern agriculture: First Edition. Publications University of Mashhad. 712 p.
Kramer, P.S. (1983). Water relations of plant. Academic press. Pp. 342-415.
Krizek, D.T., Britz, S.J., & Mirecki, R.M. (1998). Inhibitory effects of ambient levels of solar UV‐A and UV‐B radiation on growth of cv. New Red Fire lettuce. Physiologia Plantarum Journal, 103, 1-7.
Kurtzman, C.P., & Fell, J.W. (2006). Yeast Systematics and Phylogeny-Implications of Molecular Identification Methods for Studies in Ecology. In: Péter G., Rosa C. (eds) Biodiversity and Ecophysiology of Yeasts. The Yeast Handbook. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-30985-3_2
Larson, K.L. (1975). Drought injury and resistance of crop plants. In: Gupta (Ed.), U.S. Physiological Aspects of Dry Land Farming. Oxford and RHS Pub. Co., New Delhi, India, pp. 147-165.
Lister, C.E., Lancaster, J.E., & Walker, J.R. (1996). Developmental changes in enzymes biosynthesis in the skins of red and of flavonoid green apple cultivars. Journal of the Science of Food and Agriculture, 71, 313-330.
Mady, M.A. (2009). Effect of foliar application with yeast extract and zinc on fruit setting and yield of faba bean (Vicia faba L.). Journal of Biological Chememistry. Environmental Sciences, 4(2), 109-127.
Mahmoudi, H., Ksouria, R., Gharsallia, M., & Lachaa, M. (2005). Differences in responses to iron deficiency between two legumes: lentil (Lens culinaris L.) and chickpea (Cicer arietinum L.) .Plant Physiology, 162, 1237-1245.
Mahmoued, T. R. (2001). Botanical studies on the growth and germination of mahnolia (Magnolia grandiflora L.) plants. M. Sci. thesis. Faculty of Agriculture. Moshtohor, Zagazig Univ., Egypt.
Marschner, H. (1995). Mineral nutrition of higher plants. (2nd eds.) Academic Press. New York. 15-22.
Maria, A.M., Gendy, A.A., Selim, A.H., & Abd El.-All, A.M. (2008). Response of wheat plants grown under water stress in relation to Jasmonic acid. Minufiya Journal. Agricultural Research, 33(6), 1355-1375.
Mita, S., Murano, N., Akaike, M., & Nakamura, K. (1997). Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gene for beta-amylase and on the accumulation of anthocyanin those are inducible by sugars. The Plant Journal, 11,841-851.
Mohammad, S. F. (2007). Influence of Foliar Spray with Yeast Extract on Vegetative Growth, Yield of Fresh Herb, Anatomical Structure, Composition of Volatile Oil and Seed Yield Components of Basil Plant (Ocimum basilicum L.). International Journal, 3(10), 978-993.
Mohamed, S. E. (2005). Photochemical studies on common bean (Phaseolus vulgaris L.) plants as affected by foliar fertilizer and active dry yeast under sandy soil conditions. Egypt Journal Applied Scince, 20(5), 539-559.
Mohamed, A.M. (2006). Effect of Some Bio-chemical Fertilization Regimes on Yield of Maize. M.Sc. Thesis, Faculty of Agricultural & Enviromental Sciences., Zagazig Univercity., Egypt, pp. 70-177.
Moussavi-Nik, M., Rengel, Z., Hollamby, G. J., & Ascher, J. (1997). Seed manganese content ismor important than Mn fertilization for wheat, growth under Mn deficient conditions. plant nutrition for sustainable food production and environ, Plant Nutrition for Sustainable Food Production and Environment, 4, 267-268.
Nagodawithana, W.T. (1991). Yeast Technology. Universal foods corporation Milwaukee, Wisconsin. Van Nostrand Reinhold, New York, 273 p.
Naguib, N. Y., & Khalil, M. Y. (2002). Studies on The Effect of Dry Yeast, Thiamin and Biotin on The Growth and Chemical Constituents of Black Cumin (Nigella stavia). Arab Univ. Journal of Agricultural Science, 10(3), 919-937.
Naoumkina, M., Farag, M. A., Sumner, L. W., Tang, Y., Liu, C. J., & Richard, R. A. (2007). Different mechanisms for phytoalexin induction by pathogen and wound signals in Medicago truncatula. Proceedings of the National Academy of Sciences USA, 104(46), 17909-17915.
Natio, K., Nagamo, S., Fury, K., & Suzki, H. (1981). Effect of benzyladenine on RNA and protein synthesis in intact bean leaves at various stages of ageing. Plant Physiology, 52, 342-348.
Neyakani, M., & Ghorbakli, M. (2007). The effect of drought stress on growth parameteres, photosynthetic factors, content of protein, Na and K in shoot and root in two soybean cultivars. Journal of Herbs, 8(1), 17-31. (In Persian).
Olaiya, C. O. (2010). Presowing bioregulator seed treatments increase the seedling growthand yield of tomato (Solanum  lycopersicon). Journal of Plant Growth Regulation, 29(3), 349-356.
Patel, M. S., Sutar, D. M., & Kanizaria, M. V. (1993). Effect of foliar application of iron and sulfur in curing chlorosis in groundnut. Journal of the Indian Society of Soil Science, 41, 103-105.
Riccardi, F., Gazeau, D., & Vienne, M. (1998). Protein changes in response to progressive drought stress in maize. Plant Physiology, 117, 1253-1263.
Ridge, I., Murphy, P., Bell, M., & Parker, P. (1993). Plant Physiology. Biology Form and Function. In: Ridge, Irene (Ed.). Hodder and Stoughton Ltd, The Open University, UK.
Rohani, N., Nemati, H., Moghadam, M., & Ardakanian, V. (2016). Effect of humic acid on biochemical properties, anthocyanin and chlorophyll pigment content in radish cultivars under salinity stress. Plant Process and Function Journal, 6, 377-387.
Salle, A.J.  (1973). Laboratory Manual on Fundamental Principles of Bacteriology. McGraw-Hill Book Company, New York, USA.
Shekari, F. (2001). Evaluation of common bean characters to drought stress tolerance. Final Report of Research Project of Institute of Agricultural Physiology and Biotechnology of Zanjan University. (In Persian).
Solar, A., Colaric, M., Usenik, V., & Stampar, F. (2006). Seasonal variations of selected flavonoids, phenolic acids and quinines in annual shoots of common walnut (Juglans regia L.). Plant Science, 170, 453-461.
 Snedecor, G.W., & Cochran, W.G. (1980). Statistical methods. (7th Ed.). The Iowa State University press, pp. 253-254.
Upreti, K.K., & Murti, G.S.R. (2004). Effects of brassinosteroids on growth, nodulation, phytohormone content and nitrogenase activity in French bean under water stress. Biologia Plantarum, 48(3), 407-411.
Waling, L., Vark, W.V., Houba, V.J.G., & Van der Lee, J.J. (1989). Plant Analysis Procedures. Soil and Plant Analysis Part 7, Wageningen Agriculture University, the Netherlnd.
Wanas, A.L. (2002). Resonance of faba bean (Vicia faba L.) plants to seed soaking application with natural yeast and carrot extracts. Annals of Agricultural Science Moshtohor Journal, 40(1), 259-278.
Wanas, A. L. (2006). Trails for improving growth and productivity of tomato plants grown in winter. Annals of Agricultural Science Moshtohor Journal, 44(3),466-471.
Yan, Q., Shi, M., Ng, J., & Yong, J. (2006). Elicitor induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Salvia miltiorrhiza hairy roots. Plant Science, 170(4), 853-858.
Yilmaz, A., Kiz, H.E., Torun, B., Gulekin, I., Karanlk, S., Bagci, A., & Cakmak, I. (1997). Effects of different zink application methods on grain yield and zinc concentration in wheat cultivars grown on zinc deficient calcareous soils. Journal of Plant Nutrition, 20, 461-471.