همبستگی مقدار عناصر غذایی، فعالیت آنزیم های مسیر فنیل پروپانوئید و متابولیت های ثانویه در دو ژنوتیپ سیب توسرخ ایرانی و رقم رد دلیشز روی پایه های مختلف

نوع مقاله: مقاله پژوهشی

نویسندگان

1 مربی بخش تحقیقات زراعی و باغی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان سمنان (شاهرود)، سازمان تحقیقات آموزش و ترویج کشاورزی، شاهرود، ایران

2 استادیار، گروه علوم باغبانی و مهندسی فضای سبز، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

3 استاد، گروه علوم باغبانی و مهندسی فضای سبز، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

4 دانشیار بخش تحقیقات زراعی و باغی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی خراسان رضوی، سازمان تحقیقات آموزش و ترویج کشاورزی، مشهد، ایران

چکیده

به منظور بررسی نقش پایه های درختان میوه بر مقدار عناصر غذایی موجود در برگ های دو ژنوتیپ سیب توسرخ ایرانی و تعیین همبستگی عناصر غذایی با انواع متابولیت های ثانویه، این پژوهش در باغ تحقیقاتی بخش علوم باغبانی و مهندسی فضای سبز دانشگاه فردوسی مشهد طی سال های 94 تا 97 انجام شد. پایه های مورد استفاده، دو پایه پاکوتاه M9 و B9 و یک پایه کلونی سیب توسرخ بکران بودند که به صورت فاکتوریل در قالب طرح آزمایشی بلوک کامل تصادفی مورد آزمون قرار گرفتند. نتایج این پژوهش نشان داد که پایه بر مقدار نیتروژن و فسفر موجود در برگ دو ژنوتیپ سیب توسرخ و رقم رد دلیشز، و فاکتورهای پایه، پیوندک و برهمکنش پایه و پیوندک بر مقدار پتاسیم، آهن، کلسیم و روی در نمونه های برگ تیمارها تاثیر معنی داری داشتند. تاثیر پایه B9 بر مقدار تمامی عناصر برگ در همه تیمارها منفی و تاثیر پایه M9 در تمامی موارد مثبت ارزیابی شد. فعالیت آنزیم PAL با عناصر نیتروژن، پتاسیم، کلسیم، آهن و روی همبستگی مثبت معنی‌داری داشت. سنتز آنتوسیانین نیز با مقدار نیتروژن همبستگی مثبت (47‏/0) نشان داد. مقدار عناصر پتاسیم، کلسیم و آهن همبستگی بالایی با فعالیت آنزیم UFGT داشتند. مقدار فلاونوئید کل نیز با عناصر کلسیم و روی (به ترتیب 64‏/0 و 45‏/0-) همبستگی معنی داری نشان داد. که این تاثیرات را می توان به دلیل مشارکت تعدادی از این عناصر غذایی به عنوان کوفاکتور در فعالیت آنزیم ها و یا پیش ماده های ترکیبات فنلی و متابولیت های ثانویه دانست.

کلیدواژه‌ها


عنوان مقاله [English]

Correlation of Nutrient Content, Activity of Phenylpropanoid Pathway Enzymes and Secondary Metabolites in Two Iranian Red Flesh Apple Genotypes and Red delicious Cultivar on Different Rootstocks

نویسندگان [English]

  • Tahereh Parvaneh 1
  • Bahram Abedi 2
  • Gholam Hossein Davarynejad 3
  • Ebrahim Ganji Moghadam 4
1 Instructor, Horticulture Crops Research Department, Agricultural and Natural Resources Research Center of Semnan Province (Shahrood), AREEO, Shahrood, Iran
2 Assistant Professor, Department of Horticulture, Agriculture Faculty, Ferdowsi University of Mashhad, Mashhad, Iran.
3 Professor, Department of Horticulture, Agriculture Faculty, Ferdowsi University of Mashhad, Mashhad, Iran
4 Associate Professor, Horticulture Crops Research Department, Agricultural and Natural Resources Research Center, Khorasan Razavi, AREEO, Mashhad, Iran
چکیده [English]

In order to study the role of fruit tree rootstocks on nutrient content in leaves of two Iranian red flesh apple genotypes and to determine the correlation of nutrients with different secondary metabolites, this study was conducted in Research Garden of Horticultural Sciences of Ferdowsi University of Mashhad during 2017-2019. The rootstocks that used were two dwarfing rootstocks, M9 and B9 and one red flesh apple colony rootstock of Bekran which were tested in a factorial randomized complete block design. The results of this study showed that the rootstock had a significant effect on nitrogen and phosphorus content in leaf samples of two red flesh apple genotypes and Red delicious cultivar, also, rootstock, scion and their interaction had a significant effect on potassium, iron, calcium and zinc content of leaf samples. The effect of B9 on the amount of all elements of scion leaves was negative and the effect of M9 was positive in all cases. The results showed that PAL enzyme activity was positively correlated with nitrogen, potassium, calcium, iron and zinc. Anthocyanin synthesis was also positively correlated with nitrogen content (0.47). The results showed that the amount of potassium, calcium and iron were highly correlated with UFGT activity. Total flavonoid content was also significantly correlated with calcium and zinc (-0.64 and -0.45, respectively) that these effects could be due to the participation of some of these nutrients as cofactors in enzyme activity and or precursors of phenolic compounds and secondary metabolites.

کلیدواژه‌ها [English]

  • Anthocyanin
  • Red flesh apples
  • phenylalanine ammonia lyase (PAL)
  • secondary metabolites
  • Apple rootstocks
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