اثر اسید هیومیک بر شاخص‌های فیزیولوژیکی، بیوشیمیایی و عملکرد گوجه‌فرنگی تحت شرایط کم آبیاری

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

2 دانشیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

3 دانشیار، گروه مهندسی آب، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران

چکیده

به‌منظور بررسی تأثیر تنش کم­آبی و سطوح مختلف اسید هیومیک بر شاخص­های فیزیولوژیکی و عملکرد گوجه‌فرنگی، آزمایشی به‌صورت کرت‌های خردشده در قالب طرح بلوک‌های کامل تصادفی با سه تکرار، در مزرعه پژوهشی دانشگاه زنجان در سال 1395 انجام شد. تیمارهای آزمایشی شامل آبیاری در سه سطح (60، 80 و 100 درصد نیاز آبی گیاه) و محلول­پاشی اسید هیومیک در چهار سطح (صفر، 100، 200 و 300 میلی­گرم در لیتر) بودند. نتایج نشان داد که تنش کم­آبیاری به­طور معنی­داری هدایت روزنه­ای، شاخص پایداری غشا، محتوای نسبی آب برگ، کلروفیل و عملکرد بوته را کاهش داد و باعث افزایش مقدار پرولین و فعالیت آنزیم پراکسیداز شد. کاربرد اسید هیومیک فعالیت آنزیم پراکسیداز، محتوای نسبی آب برگ، مقدار پرولین، کلروفیل و شاخص پایداری غشا را تحت آبیاری نرمال و کم­آبیاری افزایش داد و باعث کاهش هدایت روزنه­ای گردید. بیشترین میزان فعالیت آنزیم پراکسیداز (207/1 واحد بر گرم وزن برگ تازه در دقیقه) و پرولین (5/11 میلی­گرم در گرم بافت تازه) با کاربرد 200 میلی­گرم در لیتر اسید هیومیک در تیمار کم­آبیاری با 60 درصد نیاز آبی حاصل شد. بیشترین میزان محتوای نسبی آب برگ (6/78 درصد) و شاخص پایداری غشا (01/70 درصد) به‌ترتیب با کاربرد 300 و 200 میلی­گرم بر لیتر اسید هیومیک در شرایط آبیاری 100 درصد نیاز آبی گیاه حاصل شد. حداکثر عملکرد بوته تحت آبیاری 100 درصد با کاربرد 200 میلی­گرم در لیتر اسید هیومیک به­دست آمد. با توجه به نتایج، کاربرد 200 میلی­گرم در لیتر اسید هیومیک جهت بهبود شاخص­های فیزیولوژیکی و عملکرد میوه گوجه­فرنگی پیشنهاد می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of humic acid on physiological and biochemical indices and yield of tomato under deficit irrigation

نویسندگان [English]

  • Shilan Aslani 1
  • Taher Barzegar 2
  • Jaefar Nikbakht 3
1 Former M.Sc. Student, Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
2 Associate Professor, Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
3 Associate Professor, Department of Water Engineering, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
چکیده [English]

In order to study the effect of water deficit stress and foliar spray of humic acid (HA) on physiological and yield traits of tomato, an experiment was carried out in a split plot based on randomized complete block design with three replications at the research filed of University of Zanjan, in 2016. Treatments consisted arrangement of three irrigation levels (starting irrigation at 100, 80 and 60 percent ETc) and 4 levels of HA (0, 100, 200 and 300 mg.l-1). The results showed that deficit irrigation significantly decreased stomatal conductance, membrane stability index, leaf relative water chlorophyll a and b content and plant yield, and increased the proline content and proxidase activity. Treatment of HA enhanced proxidase activity, leaf relative water content, proline, chlorophyll, membrane stability index and plant yield under normal and deficit irrigation, and decreased stomatal conductance. The highest peroxidase activity (1.207 uints.g-1FW.Min-1) and proline content (11.5 mg.gFW-1) was obtained with treatment of HA 200 mg L-1 under irrigation 60 percent ETc. Also, maximum leaf relative water content (78.6 percent) and membrane stability index (70.01 percent) was achieved in HA 300 and 200 mg L-1, respectively under irrigation 100 percent ETc. The maximum plant yield was obtained with application of HA 200 mg L-1 under irrigation 100 %ETc. According to the results, application of HA 200 mg L-1 can be proposed to improve physiological traits and increase fruit yield.

کلیدواژه‌ها [English]

  • Foliar spray
  • Membrane stability index
  • proline
  • Proxidase
  • Stomatal conductance
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