نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه مهندسی علوم باغبانی و فضای سبز، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

2 استاد، گروه مهندسی علوم باغبانی و فضای سبز، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

3 دانشیار، گروه مهندسی علوم باغبانی و فضای سبز، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

4 استادیار، پژوهشکده پسته، سازمان تحقیقات، آموزش و ترویج کشاورزی استان سمنان، مرکز دامغان، ایران

چکیده

گیاهان هنگام مواجه شدن با انواع تنش‌های محیطی از سیستم آنتی‌اکسیدانی جهت مقابله با عوارض ناشی از تنش‌ استفاده می‌کنند. در این تحقیق به‌منظور بررسی اثر تنش شوری ناشی از افزایش کلرید سدیم بر سیستم آنتی‌اکسیدانی و برخی خصوصیات فیزیولوژیک دانهال‌های پسته (بادامی‌ریز زرند، قزوینی، سرخس، آتلانتیکا، UCB1 و دورگ‌های cv. BadamiRiz-e--Zarand × P. Integerrima ، Pistacia. vera، cv. Sharakhs× P. Integerrima P. vera P. atlantica Desf× P. Integerrima,) آزمایشی گلخانه‌ای به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در بستر کوکوپیت و پرلایت انجام‌شد. عوامل آزمایش شامل تنش شوری (صفر، 75، 150 و 225 میلی‌مولار) و پایه بودند. اعمال تنش در سال دوم به مدت نه هفته انجام گردید. در پایان آزمایش برخی خصوصیات فیزیولوژیکی و بیوشیمیایی دانهال‌ها اندازه‌گیری شدند. نتایج نشان داد که با افزایش سطح شوری، سطح برگ و کلروفیل کل در گیاهان مورد مطالعه کاهش یافتند. پایه آتلانتیکا بیشترین و پایه بادامی‌ریز زرند کمترین کاهش را در این خصوص نشان دادند. همچنین با افزایش سطح شوری، میزان پرولین برگ، فنول کل و فعالیت آنزیم‌های آنتی‌اکسیدان افزایش یافتند. بیشترین افزایش فعالیت آنزیم‌های آنتی‌اکسیدان در دورگ بادامی‌ریز زرند× اینتگریما، پایه‌های بادامی‌ریز زرند و قزوینی مشاهده شد. با توجه به نتایج این پژوهش به نظر می‌رسد دورگ بادامی‌ریز زرند× اینتگریما، پایه‌های بادامی‌ریز زرند و قزوینی توانایی بیشتری در حفظ پروتئین‌های برگ داشته و تحمل بالاتری نسبت به تنش شوری دارند.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of Biochemical traits in some Pistachio Rootstocks under salt stress to select tolerant Rootstocks

نویسندگان [English]

  • Khiermohammad Mohammadi 1
  • Ali Ebadi 2
  • Mohammad Ali Askari Sarchishmeh 3
  • Mohammad Reza fattahi Moghaddam 2
  • Hossein Hokmabadi 4

1 Ph.D. Candidate, Department of Horticultural Science and Landscape Engineering, Faculty of Agricultural Science and Engineering, College of Agricultural and Natural Resources, University of Tehran, Karaj, Iran

2 Professor, Department of Horticultural Science and Landscape Engineering, Faculty of Agricultural Science and Engineering, College of Agricultural and Natural Resources, University of Tehran, Karaj, Iran

3 Associate Professor, Department of Horticultural Science and Landscape Engineering, Faculty of Agricultural Science and Engineering, College of Agricultural and Natural Resources, University of Tehran, Karaj, Iran

4 Assistant Professor, Pistachio Research center, Agricultural Research Education and Extention Organization of Semnan Province, Dameghan center, (Iran).

چکیده [English]

Plants employ antioxidant mechanism to face different stresses. In this research work the effect of salinity stress, caused by increasing sodium chloride, on the antioxidative system and some physiological characteristics of selected pistachio seedlings (Badami-Riz-e- Zarand, Qazvini, Sarakhs, Atlantica UCB1, and Hybrids of Pistacia. vera cv. Badami-Riz-e-Zarand × P. Integerrima, P. vera cv. Sharakhs× P. Integerrima, P. atlantica Desf. × P. Integerrima ) was studied. A greenhouse experiment was conducted as a factorial based on a completely randomized design with three replications in Cocopit and Perlite media. Treatments consisted of four salinity levels (0, 75, 150 and 225 mM NaCl) and above mentioned Root stocks. Using salt in nutrient solution was carried out in the second year of seedling growth for nine weeks. At the end of experiment, some physiological and biochemical traits of seedlings were measured. Results showed that, leaf area and total chlorophyll contents decreased in all treated plants. Under the salinity stress, a higher reduction in the observed traits was noticed in the Atlantica rootstock while less effect on the Badami-Riz-e-Zarand was noticed. Also, it has been found that by increasing salinity levels the levels of proline, total phenol content and antioxidant enzymes activity were higher in Badami-Riz-e-Zarand × P. Integerrima, Badami-Riz-Zarand and Qazvini compared to others. It seems that Hybrid of Badami-Riz-e-Zarand × P. Integerrima, Badami-Riz-Zarand and Qazvini rootstocks had more ability to sustain leaf water and proteins and therefore, were more resistant to salinity stress.

کلیدواژه‌ها [English]

  • Atlantic
  • Enzymatic activity
  • Leaf area
  • proline
  • Root stock
  • Salt tolerance
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