مطالعه واکنش‌های مورفوفیزیولوژیکی گیاه خرفه به متیل‌جاسمونات تحت تنش شوری

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار، مرکز تحقیقات گیاهان دارویی دانشگاه شاهد، تهران، ایران

2 دانش‌آموخته کارشناسی ارشد، فضای سبز شهرداری تهران، تهران، ایران

3 دانش‌آموخته کارشناسی ارشد، مرکز تحقیقات گیاهان دارویی دانشگاه شاهد، تهران، ایران.

چکیده

به‌منظور بررسی واکنش‌های مورفوفیزیولوژیکی گیاه خرفه (Portulaca oleracea L.) به متیل‌جاسمونات تحت تنش شوری با کلرید‌سدیم آزمایشی به‌صورت کرت‌های خرد‌شده با دو فاکتور بر پایه طرح کاملا تصادفی با سه تکرار در مرکز تحقیقات گیاهان دارویی دانشگاه شاهد واقع در جنوب تهران در سال 1396 انجام شد که در آن غلظت‌های شوری با 4 سطح (0، 3، 6، 9 دسی‌زیمنس) به‌عنوان فاکتور اصلی و غلظت‌های متیل‌جاسمونات با 4 سطح (0، 25/0، 5/0 و 75/0 میلی‌مولار) به‌عنوان فاکتور فرعی مورد بررسی قرار گرفتند. نتایج نشان داد که با افزایش غلظت شوری شاخص‌های رشد از قبیل طول ریشه، وزن‌تر ریشه، کلروفیل b، کلروفیل کل و میزان فعالیت آنزیم سوپر‌اکسید‌دیسموتاز کاهش پیدا کرد، در حالیکه با افزایش غلظت شوری مقدار پرولین برگ، میزان فعالیت مالون‌دی‌آلدئید و کاتالاز افزایش یافت. با اعمال تیمار متیل‌جاسمونات تحت تنش شوری میزان کاهش شاخض‌های رشد و رنگیزه‌های فتوسنتزی تعدیل پیدا کرد. بیشترین ارتفاع بوته، تعداد شاخه جانبی، تعداد برگ، وزن‌تر و خشک اندام هوایی و پرولین در غلظت شش دسی‌زیمنس بر متر شوری و 5/0 میلی‌مولار متیل‌جاسمونات بدست آمد، همچنین با افزایش سطح متیل‌جاسمونات میزان پرولین و میزان فعالیت کاتالاز افزایش یافت ولی با افزایش غلظت متیل‌جاسمونات میزان فعالیت مالون‌دی‌آلدئید و سوپر‌اکسید‌دیسموتاز در برگ کاهش یافت. بنابراین میتوان نتیجه‌گیری کرد که تحمل گیاه خرفه به شوری حداکثر شش دسی‌زیمنس بر متر شوری می‌باشد و مصرف مقدار پائین متیل‌جاسمونات (5/0 میلی‌مولار) منجر به افزایش حدود 7/30 درصد در عملکرد و بهبود شاخص‌های فیزیولوژیک خرفه می‌شود.

کلیدواژه‌ها


عنوان مقاله [English]

Study of Morpho-physiological responses of Purslane to methyl jasmonate under salinity stress conditions

نویسندگان [English]

  • Dariush Talei 1
  • Reza Sharifi 2
  • Mahdi Pirsalehi 3
1 Assistant Professor, Medicinal Plants Research Center, Shahed University, Tehran, Iran.
2 Former M.Sc. Student, Green Space of Tehran Municipality, Tehran, Iran.
3 Former M.Sc. Student, Medicinal Plants Research Center, Shahed University, Tehran, Iran.
چکیده [English]

In order to investigate the morpho-physiological responses of Purslane plant to methyl jasmonate under salinity stress a split plot based on a completely randomized design with two factors and three replications was carried out in medicinal plants research center, Shahed University, Tehran during 2017. The factors were, salinity with four levels (0, 3, 6 and 9 dS/m) as a main factor and methyl jasmonate with four levels (0, 0.25, 0.5 and 0.75 mM) as a sub factor. The results showed that by increasing salinity levels, the growth indices such as root length, fresh weight of root, chlorophyll b, total chlorophyll and the amount of superoxide dismutase decreased, while by increasing the salinity levels the proline content and the activity of catalase and MDA in the leaf increased. Applying methyl jasmonate under salinity stress reduced the growth indices and photosynthetic pigments. The highest number of branches, number of leaves and chlorophyll b was obtained at 6 dS/m salinity and 0.5 mM methyl jasmonate. By increasing methyl jasmonate level, the proline content and the activity of CAT increased, while the amount of MDA and SOD enzymes decreased. Therefore, it can be concluded that the is the tolerance of Portulaca oleracea plant to salinity was up to six dS/m, and consuming low amounts of methyl jasmonate can improve yield and physiological indices in Purslane.

کلیدواژه‌ها [English]

  • Catalase
  • Malondialdehyde
  • morphological traits
  • Physiological traits
  • superoxide dismutase

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