ارزیابی کمون ثانویه و رفتار جوانه‌زنی بذر لاین‌ها و ارقام کلزا

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه زراعت، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 دانشیار، گروه زراعت، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

3 استادیار، مؤسسة تحقیقات کشاورزی و منابع طبیعی استان گلستان، گرگان، ایران

4 استاد، گروه زراعت، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

5 دانشیار، گروه زیست‌شناسی، دانشگاه گلستان، گرگان، ایران

چکیده

کمون ثانویه یکی از اصلی‌ترین دلایل پایداری بانک بذر در خاک است. بذرهای کلزای موجود در بانک بذر خاک پس از رفع کمون ثانویه جوانه زده و می‌توانند به پراکنش ناخواسته ژن‌های هدف به دیگر گیاهان منجر شوند. در این مطالعه کمون ثانویه با استفاده از پلی‏اتیلن‏گلایکول 6000 به‏مدت 14 روز تحت شرایط آزمایشگاهی در 41 لاین و 5 رقمکلزا القا و درصد کمون ثانویه بذرها ثبت شد. این مطالعه در قالب طرح کاملا تصادفی انجام شد. تمامی لاین‌ها و ارقام در شرایط مطلوب جوانه‌زنی، از جوانه‌زنی بسیار بالایی برخوردار (بالای 94 درصد) و پس از القای کمون ثانویه در پنج گروه بسیارکم، کم، متوسط، زیاد و بسیار زیاد با استفاده از تجزیة کلاستر، دسته‌بندی شدند. پنج لاین در گروه بسیارکم و دو لاین در گروه بسیار زیاد و بقیة لاین‌ها در گروه متوسط و کم قرار گرفتند. پنج رقم (RGS003، زرفام، هایولا 401، هایولا 308 و هایولا 50) از کمون ثانویه متوسط (40-60 درصد) برخوردار بودند که بیانگر عدم توجه اصلاح‌گران به بحث مهم کمون ثانویة آن‌ها طی روند اصلاح آن‌ها بوده است. دسته‌بندی لاین‌ها براساس سطح کمون ثانویه به تولیدکنندگان بذر کمک می‌کند تا لاین‌هایی را انتخاب کنند تا علاوه بر عملکرد و دیگر ویژگی‌های مهم در امر تولید بذر، از کمون ثانویه پایین‌تری برخوردار باشند تا از دیدگاه تولید بذر مشکلات ناشی از آن‌ها در محصول بعد به حداقل برسد.

کلیدواژه‌ها


عنوان مقاله [English]

Assessment of germination and secondary dormancy behaviours of lines and cultivars of canola

نویسندگان [English]

  • Ali Shayanfar 1
  • Farshid Ghaderifar 2
  • Rahmatollah Behmaram 3
  • Afshin Soltani 4
  • Hamidreza Sadeghipour 5
1 Gorgan University of Agricultural and Natural Resources
2 Gorgan University of Agricultural and Natural Resources
3 Research Institute of Agriculture and Natural Resources of Golestan Province
4 Gorgan University of Agricultural and Natural Resources
5 Golestan University, Biology Department
چکیده [English]

Secondary seed dormancy is known as the major reason for seed persistence of canola (Brassica napusL). Volunteer’s rapeseeds emerging from the soil seed bank can lead to unwanted gene dispersal to other plants after breaking secondary seed dormancy. At the current study, secondary dormancy was induced in 41 lines and 5 cultivars of canola under laboratory condition with using polyethylene glycol 6000, during 14 days and secondary seed dormancy recorded. This study was conducted as a randomized complete design. High germination percentage was observed at the all lines and cultivars (higher than 94%), and they were classified at five groups included very low, low, medium, high and very high secondary dormancy using cluster analysis. Among different lines, five genotypes were included at the very low group and two genotypes were included at the very high group. The other lines were placed in average and low groups. It was observed that five varieties (RGS003, Zarfam, Hyola401, Hyola308 and Hyola50) had average secondary dormancy (40-60%) that was related to breeding ignorance about secondary dormancy during seed production process. Lines classification based on different levels of secondary dormancy helps seed producers to select lines with low levels of secondary dormancy along with high yield and other characteristics, in order to deal with problems in seed producing process.

کلیدواژه‌ها [English]

  • Canola
  • dormancy
  • seed bank
  • seed production
  • Weeds

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