تأثیر متیل جاسمونات بر فعالیت آنزیم‌های آنتی‏اکسیدان و محتوای عناصر غذایی لوبیا چشم‌بلبلی تحت تنش شوری

نوع مقاله: مقاله پژوهشی

نویسنده

دانشیار، گروه زراعت، واحد یادگار امام خمینی (ره) شهر ری، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

به‌منظور بررسی نقش متیل جاسمونات در تحمل به تنش شوری در لوبیاچشم‌بلبلی آزمایشی گلدانی به‌صورت فاکتوریل در قالب طرحی کاملاً تصادفی با سه تکرار در سال 1394 در شهرستان ری انجام شد. تیمارها شامل سه سطح خیساندن بذرها در محلول متیل جاسمونات (صفر، 25 و 50 میکرو مولار) به‌مدت 20 ساعت و سه سطح شوری آب آبیاری (صفر، 50 و 100 میلی‌مولار کلرید سدیم) بود. نتایج نشان داد با افزایش سطح شوری، پراکسایش چربی‌ها، فعالیت آنزیم‌های آنتی‌اکسیدان (سوپراکسید دیسموتاز، کاتالاز، اسکوربات پراکسیداز و گلوتاتیون ردکتاز) و غلظت کلر در برگ افزایش می‌یابد، در حالی ‏که غلظت نیتروژن، کلسیم و منیزیم، همچنین نسبت پتاسیم به سدیم کاهش یافت. این تغییرات در نهایت، به کاهش عملکرد دانه انجامید. از سوی دیگر، تیمار بذر با متیل جاسمونات در شرایط عدم تنش تأثیر معناداری بر صفات اندازه‌گیری‌شده نداشت، ولی کاربرد آن (به‌ویژه غلظت 50 میکرومولار) تحت تنش شوری، موجب فعالیت بیشتر آنزیم‌های آنتی‌اکسیدان، کاهش پراکسایش چربی‌ها، کاهش غلظت کلر در برگ، افزایش غلظت نیتروژن، کلسیم و منیزیم، همچنین نسبت پتاسیم به سدیم شد. در نتیجه موجب بهبود عملکرد شد. براساس نتایج این پژوهش، متیل جاسمونات در القای تحمل به شوری از طریق افزایش فعالیت آنزیم‌های آنتی‌اکسیدان و تغییر در ترکیب عناصر غذایی در لوبیاچشم‌بلبلی نقش مهمی ایفا می‌کند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of methyl jasmonate on antioxidant enzymes activity and nutrients content of cowpea under salinity stress

نویسنده [English]

  • Omid Sadeghipour
چکیده [English]

In order to investigate the role of methyl jasmonate on salinity tolerance of cowpea, a factorial pot experiment was conducted in the basis of completely randomized design with three replications in the Rey region in 2015. Treatments included three levels of seed soaking in methyl jasmonate solution (0, 25 and 50 µM) for 20 hours and three levels of salinity (0, 50 and 100 mM NaCl). The results showed that by increasing salinity levels, lipids peroxidation, antioxidant enzymes activity (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase) and Cl- concentration in the leaves increased, while the concentration of N, Ca2+ and Mg2+ decreased as well as K+/Na+ ratio. These changes eventually led to decreasing seed yield. Seed treatment with methyl jasmonate had not significantly effect on any measured traits under normal conditions, however this treatment (especially 50 µM) under salinity stress conditions caused further activity of antioxidant enzymes, declining lipids peroxidation, reducing the concentration of Cl- in the leaves, increasing the concentration of N, Ca2+ and Mg2+ as well as K+/Na+ ratio, and finally improved seed yield. These findings suggest that methyl jasmonate plays a pivotal role in inducing salinity tolerance of cowpea plants via enhancing antioxidant enzymes activity and altering nutrients composition.

کلیدواژه‌ها [English]

  • lipids peroxidation
  • plant growth regulators
  • seed pre-treatment
  • Sodium Chloride
  • yield
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