اثر 1- متیلسیکلوپروپان و اتیلن بر صفات فیزیولوژیکی و فعالیت آنزیم های آنتی اکسیدانی گله ای شاخه بریدنی میخک ‘گرند اسلم’

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد علوم باغبانی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

2 استادیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

به منظور بررسی اثر تیمار 1- متیلسیکلوپروپان و اتیلن بر حفظ کیفیت و افزایش عمر گلجایی گلهای میخک رقم ‘گرنداسلم’ آزمایشی در قالب طرح کاملاً تصادفی با سه تکرار در آزمایشگاه فیزیولوژی پس از برداشت دانشگاه تربیت مدرس در سال 93-1392 انجام شد. ابتدا گلهای شاخهبریدنی با چهار سطح صفر، 5/0، 1 و 5/1 میکرولیتر بر لیتر 1- متیلسیکلوپروپان به مدت 24 ساعت تیمار شدند و سپس به مدت 16 ساعت در معرض غلظت یک میکرولیتر بر لیتر اتیلن قرار گرفتند.تیمار 1- متیلسیکلوپروپان اثر معنی‌داری بر عمر گلجایی، ویژگی­های بیوشیمیایی و میزان فعالیت آنزیم‌های آنتی‌اکسیدانت داشت. بیشترین عمر گلجایی و مقدار کلروفیل برگ و آنتوسیانین‌های گلبرگ مربوط به تیمار یک میکرولیتر بر لیتر 1- متیلسیکلوپروپان بود، هرچند که با تیمار 5/1 میکرولیتر بر لیتر 1- متیل‌سیکلوپروپان تفاوت معنی‌داری نداشت. بیشترین فعالیت آنزیم پراکسیداز تحت تیمار 1- متیلسیکلوپروپان با غلظت یک میکرولیتر بر لیتر مشاهده شد، درحالی‌که بیشترین و کمترین فعالیت آنزیم کاتالاز و سوپراکسید دیسموتاز به‌ترتیب در تیمار 5/1 میکرولیتر بر لیتر و تیمار شاهد مشاهده شد. به‌طورکلی، نتایج تحقیق حاضر نشان داد که 1- متیل‌سیکلوپروپان به عنوان یک بازدارنده عمل اتیلن سبب افزایش عمر گلجایی و فعالیت آنزیم­های آنتی‌اکسیدانی در گل شاخه‌بریدنی میخک رقم ‘گرند اسلم’ گردید.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of 1-Methylcyclopropene (1-MCP) and Ethylene on physiological characteristics and antioxidant activities of cut carnation cv. ‘Grand Slam’

نویسندگان [English]

  • Azam Ranjbar 1
  • Nourollah Ahmadi 2
1 M.Sc. Former Student, Department of Horticultural Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
2 2Assistant Professor, Department of Horticultural Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
چکیده [English]

This research was conducted to evaluate the effects of 1-MCP and ethylene on extending the display quality and increasing vase life of cut carnation flowers in a completely randomized design with three replicationsat the laboratory of the postharvest physiology, Tarbiat Modares Universityin 2014. First, cut flowers were treated with 0, 0.5, 1 and 1.5 µl l-1 of 1-MCP for 24 h and then exposed to 1 µl l-1 ethylene for 16 h. The results showed that 1-MCP had significant effect on the physiological and biochemical characteristics and the activity of antioxidant enzymes. Maximum of vase life and the amount of chlorophyll and anthocyanin were revealed in samples treated with 1 µl l-1 1-MCP. Also, the highest peroxidase enzyme activity was observed in treatment of 1 µl l-1 1-MCP which had significant difference with other treatments. The highest and lowest catalase and superoxide dismutase activities were found in 1.5 µl l-1 1-MCP and control, respectively. In conclusion, 1-MCP treatment resulted in extending vase life and increasing activity of antioxidant enzymes in cut carnation cv. ‘Grand Slam’ by suppression of ethylene action.

کلیدواژه‌ها [English]

  • Chlorophyll
  • Peroxidase
  • Senescence
  • superoxide dismutase
  • Vase solution

1 . Ahmadi N, Mibus H and Serek M (2008) Isolation of an ethylene induced putative nucleotide laccase in miniature roses (Rosa hybrida L). Journal of Plant Growth Regulation. 27: 320-330.

2 . Ahmadi N, Mibus H and Serek M (2009) Characterization of ethylene-induced organ abscission in F1 breeding lines of miniature roses (Rosa hybrida L.). Postharvest Biology and Technology. 52: 260-266.

3 . Asil MH, Karimi M and Zakizadeh H (2013) 1-MCP improves the postharvest quality of cut spray carnation (Dianthus caryophyllus L.)‘Optima’flowers. Horticulture, Environment, and Biotechnology. 54(1): 58-62.

4 . Barth C, De Tullio  M and Conklin PL (2006) The role of ascorbic acid in the control of flowering time and the onset of senescence. Journal of Experimental Botany. 57(8): 1657-1665.

5 . Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 72: 248-254.

6 . Buchanan-Wollaston V (1997) The molecular biology of leaf senescence. Journal of Experimental Botany.48(2): 181-199.

7 . Cakmak I and Horst WJ (1991) Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiologia Plantarum. 83: 463-468.

8 . Cameron AC and Reid MS (2001) 1-MCP blocks ethylene-induced petal abscission of Pelargonium peltatum but the effect is transient. Postharvest Biology and Technology. 22: 169-177.

9 . Chance B and Maehly A (1955) Methods in enzymology. SP Colowick, NO Kaplan, Eds. 2: 764 pp.

10 . Chutichudet P, Chutichudet B and Boontiang K (2010b) Effect of 1-MCP fumigation on vase life and other postharvest qualities of siam tulip (Curcuma aeruginosa Roxb.) cv. laddawan. International Journal of Agricultural Research. 5(1): 1-10.

11 . Daneshi Nergi MA and Ahmadi N (2014) Effects of 1-MCP and ethylene on postharvest quality and expression of senescence-associated genes in cut rose cv. Sparkle. Scientia Horticulturae. 166: 78-83.

12 . Djanaguiraman M, Prasad P and Al-Khatib K (2011) Ethylene perception inhibitor 1-MCP decreases oxidative damage of leaves through enhanced antioxidant defense mechanisms in soybean plants grown under high temperature stress. Environmental and Experimental Botany.71(2): 215-223.

13 . Ebeles FB, Morgan PW and Saltveit ME (1992) Ethylen in plant biology. 2nd ed. Academic press, NewYork.

14 . Francis FJ (1989) Food colorant. Anthocyanins. Critical Reviews in Food Science and Nutrition. 28: 273-314.

15 . Gao C, Wang Y, Liu G, Wang C, Jiang J and Yang C (2010) Cloning of ten peroxidase (POD) genes from Tamarix  hispida and characterization of their responses to abiotic stress. Plant Molecular Biology Reporter. 28(1): 77-89.

16 . Hassan F and Ali E (2014) Protective effects of 1-methylcyclopropene and salicylic acid on senescence regulation of gladiolus cut spikes. Scientia Horticulturae. 179: 146-152.

17 . Hershkovitz V, Saguy SI and Pesis E (2005) Postharvest application of 1-MCP to improve the quality of various avocado cultivars. Postharvest Biology and Technology. 37(3): 252-264.

18 . Jiang YM and Chen F (1995) A study on polyamine change and browning of fruit during cold storage of litchi (Litchi chinensis Sonn.). Postharvest Biology and Technology. 5(3): 245-250.

19 . Jiang Y,  Duan X, Joyce D, Zhang Z and Li J (2004) Advance in understanding of enzymatic browning in harvested litchi fruit. Food Chemistry. 88(3): 443-446.

20 . Karimi  M (2014) Change in ethylene production and ACC content of potted carnation in response to anti-ethylene treatments. International Journal of Biosciences. 4(8): 116-123.

21 . Krizek DT, Kramer GF, Upadhyaya A and Mirecki RM (1993) UV-B response of cucumber seedlings grown under metal halide and high pressure sodium/deluxe lamps. Physiologia Plantarum. 88: 350-358.

22 . Larrigaudiere C, Vilaplana R, Soria Y and  Recasens I (2004) Oxidative behaviour of Blanquilla pears treated with 1‐methylcyclopropene during cold storage. Journal of the Science of Food and Agriculture. 84(14): 1871-1877.

23 . Li Z, Wang L, Wang W and Zhu Y (2007) Physiological effect and application of 1-MCP on delaying fruit senescence. Plant Physiology Communications. 43: 201-206.

24 . Ma N, Tan H,  Liu X, Xue J, Li Y and Gao J (2006) Transcriptional regulation of ethylene receptor and CTR genes involved in ethylene-induced flower opening in cut rose (Rosa hybrida) cv. Samantha. Journal of Experimental Botany. 57(11): 2763-2773.

25 . Mayak S, Legge RL and Thompson JE (1983) Superoxide radical production by microsomal membranes from senescing carnation flowers: an effect on membrane fluidity. Phytochemistry. 22(6): 1375-1380.

26 . Miao L and St Clair DK (2009) Regulation of superoxide dismutase genes: implications in disease. Free Radical Biology and Medicine. 47(4): 344-356.

27 . Richardson AD, Duigan SP and Berlyn GP (2002) An evaluation of noninvasive methods to estimate foliar chlorophyll content. New Phytologist.153: 185-194.

28 . Sahebjamei H, Abdolmaleki P and Ghanati F (2007) Effects of magnetic field on the antioxidant enzyme activities of suspension cultured tobacco cells. Bioelectromagnetics. 28: 42-47.

29 . Seglie L, Martina K, Devecchi D, Roggero C, Trotta F and Scariot V (2011) The effects of 1-MCP in cyclodextrin-based nanosponges to improve the vase life of Dianthus caryophyllus cut flowers. Postharvest Biology and Technology. 59: 200-205.

30 . Serek M, Jones RB and Reid MS (1994) Role of ethylene in opening and senescence of Gladiolus sp. flowers. JournalAmerican Society for Horticultural Science. 119: 1014-1019.

31 . Serek M, Praducki A and Sisler EC (1998). Inhibitors of ethylene action affect final quality and rooting of cuttings before and after storage. HortScience. 33: 153-155.

32 . Serek M and Sisler EC (2001) Efficacy of inhibitors of ethylene binding in improvement of the postharvest charactericts of potted flowering plants. Postharvest Biology and Technology. 23: 161-166.

33 . Singh K (1994) Effects of spermidine, IAA, ACC and ethylene on petal longevity in carnation (Dianthus caryophyllus L.). Phyton. 34: 309-313.

34 . Singh H, Hallan V, Raikhy G, Kulshrestha S, Sharma M, Ram R, Garg I and Zaidi A (2005) Characterization of an Indian isolate of carnation mottle virus infecting carnations. Current Science. 88: 594-601.

35 . Staskawicz BJ, Ausubel FM, Baker BJ, Ellis JG and Jones JD (1995) Molecular genetics of plant disease resistance. Science-New York Then Washington. Pp. 661-661.

36 . Spanou CI, Veskoukis AS, Stagos D, Liadaki K, Aligiannis N, Angelis A, Skaltsounis AL, Anastasiadi M, Haroutounian SA and Kouretas D (2012) Effects of Greek legume plant of extracts on xanthine oxidase, catalase and superoxide dismutase activities. Journal of Physiology and Biochemistry. 68(1): 37-45.

37 . Yang SF and Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants.  Annual Review of Plant Physiology. 35: 155-189.

38 . Zhang  Z, Pang X, Ji Z and Jiang Y (2001) Role of anthocyanin degradation in litchi pericarp browning. Food Chemistry.75(2): 217-221.

39 . Zhang P, Zhang M, Wang S and Wu Z (2012) Effect of 1-methylcyclopropene treatment on green asparagus quality during cold storage. International Agrophysics. 26(4): 407-411.

40 . Zhou Q, Ma C, Cheng S, Wei B, Liu X and Ji S (2014) Changes in antioxidative metabolism accompanying pitting development in stored blueberry fruit. Postharvest Biology and Technology. 88: 88-95.

41 . Xie M, Zhang J and Xie J (2003) Relationships between some physio-biochemical changes and senescence during storage in bitter gourd. Acta Botanica Boreali-Occidentalia Sinica.24(4): 716-719.