ارزیابی فعالیت آنزیم‌های آنتی اکسیدان در واکنش به تلقیح میکوریزایی در گندم تحت تنش شوری

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دکتری تخصصی فیزیولوژی گیاهان زراعی، گروه زراعت و اصلاح نباتات، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، تهران - ایران

2 استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه یاسوج، یاسوج - ایران

چکیده

تحقیق حاضر با هدف ارزیابی نقش قارچ میکوریزایی بر فعالیت آنتی­اکسیدانی گندم تحت تنش شوری، در گلخانه دانشکده کشاورزی و منابع طبیعی دانشگاه تهران، در سال 1390 انجام شد. آزمایش به صورت فاکتوریل و در قالب طرح بلوک کامل تصادفی در سه تکرار اجرا گردید. تیمارهای آزمایشی عبارت از سه سطح تنش شوری شامل شاهد (بدون تنش)، 60 و 120 میلی­مولار نمک کلرید سدیم و دو سطح تلقیح میکوریزایی (تلقیح و عدم تلقیح قارچ میکوریزاییGlomusmosseae) بود. صفات مورد ارزیابی شامل طول ریشه و اندام هوایی، وزن خشک ریشه و اندام هوایی، درصد کلونیزاسیون ریشه و فعالیت آنزیم­های آنتی­اکسیدان سوپراکسیددیسمیوتاز، کاتالاز و گایاکول پراکسیداز بود. نتایج حاصل نشان­دهنده اثرات بازدارنده تنش شوری بر رشد گیاه گندم بود، به نحوی که با افزایش شدت تنش شوری طول و وزن خشک اندام هوایی و ریشه به میزان قابل ملاحظه­ای کاهش یافت. به­کارگیری تیمار میکوریزایی موجب بهبود رشد اندام هوایی و ریشه بوته­های گندم در شرایط تنش گردید. همچنین، تنش شوری موجب کاهش درصد کلونیزاسیون ریشه و افزایش فعالیت آنزیم­های آنتی­اکسیدان مورد ارزیابی گردید. اعمال تیمار میکوریزایی نقش مؤثری در ارتقاء رشد و فعالیت آنزیم‌های آنتی‌اکسیدان گندم به ویژه در شرایط تنش شوری داشت. هرچند اثر متقابل شوری و میکوریزا برای آنزیم­های کاتالاز و سوپراکسیددیسمیوتاز در ریشه و برای آنزیم گایاکول پراکسیداز در اندام هوایی و ریشه معنی­دار نبود.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Arbuscular Mycorrhizal Fungus (AMF) on antioxidant enzyme activities in salt-stressed wheat

نویسندگان [English]

  • Omid Younesi 1
  • Ali Moradi 2
1 Ph.D. in Crop Physiology, Department of Agronomy and Plant Breeding, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Tehran - Iran
2 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Yasuj University, Yasuj - Iran
چکیده [English]

This study investigated the influence of inoculation with an arbuscular mycorrhizal fungus (AMF), Glomus mosseae (Nicol & Gerd.) on growth and antioxidant enzyme activities (SOD, CAT, GUPX) in shoots and roots of wheat (Triticum aestivum L.) affected by three different levels of salt stress. The experiment was arranged as a factorial in Randomized Complete Block Design (RCBD) with three replications. Experimental treatments including: three levels of salinity stress (0 (control), 60 and 120 µm) and two levels of inoculation (inoculation and non inoculation). Salinity decreased wheat growth, regardless of the mycorrhizal treatment and the salt stress level. The plants inoculated with AMF had significantly greater shoot biomass than the control plants at all salinity levels. However, the results pointed out that salinity had inhibitory effects on mycorrhizal infection. The highest mycorrhizal infection was observed in the control plants. Increasing salinity stress raised significantly the antioxidant enzyme activities, including those of total SOD, GUPX and CAT, of wheat compared to their respective non-stressed controls. The AMF induced a higher increase in these antioxidant enzymes in response to severe salinity. Inoculation with AMF could serve as a useful tool for alleviating salinity stress in salt-sensitive plants. However, Analysis of variance indicated that there was not significant interaction between salt and mycorrhizal inoculation on SOD and CAT of roots. Also, there was not significant interaction between salt and mycorrhizal inoculation on GUPX in both shoots and roots.

کلیدواژه‌ها [English]

  • Arbuscular Mycorrhizal Fungi
  • Cat
  • DUPX
  • salinity
  • SOD
  • Wheat

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