نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناس ارشد گروه باغبانی، دانشکدة کشاورزی، دانشگاه بوعلی سینا، همدان، ایران
2 استادیار گروه مهندسی فضای سبز، دانشکدة کشاورزی، دانشگاه ملایر، ملایر، ایران
3 دانشیار، گروه باغبانی، دانشکدة کشاورزی، دانشگاه بوعلی سینا، همدان، ایران
4 کارشناس ارشد، گروه باغبانی، دانشکدة کشاورزی، دانشگاه بوعلی سینا، همدان، ایران
5 استادیار، گروه مهندسی فضای سبز، دانشکدة کشاورزی، دانشگاه ملایر، ملایر، ایران
چکیده
به‌منظور ارزیابی اثر محلولپاشی متیل‌جاسمونات (غلظت‌های صفر (شاهد)، 50، 100 و 200 میکرومولار) بر درصد نشت یونی، شاخص سرمازدگی، محتوای آب نسبی، غلظت کربوهیدرات‌های محلول، پرولین و محتوای کلروفیل برگ دانهالهای خیار گلخانه‌ای رقم ’نگین‘ در مرحلة چهار تا شش‌برگی، آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با پنج تکرار، در یکی از گلخانه‌های دانشگاه بوعلی سینا، در اواخر تابستان 1392 انجام گرفت. محلولپاشی متیل جاسمونات دو بار در روز روی دانهالهای خیار صورت پذیرفت. دو روز بعد از محلولپاشی، دانهال‌ها از دمای 25 درجة سانتی‌گراد به اتاقک سرماساز منتقل شدند و چهار ساعت تحت تیمارهای سرمایی 15، 10 و 5 درجة سانتی‌گراد قرار گرفتند. با کاهش دما، درصد نشت یونی برگ در تمام دانهال‌ها افزایش یافت. با این‌حال، در گیاهان تیمارشده با متیل‌جاسمونات، به‌ویژه با غلظت200 میکرومولار، نشت یونی کمتری مشاهده شد. کاهش دما از 15 تا 5 درجة سانتی‌گراد سبب افزایش غلظت کربوهیدرات‌های محلول و پرولین برگ در تمام دانهال‌ها شد که این افزایش در گیاهان تیمارشده با متیل جاسمونات، به‌ویژه با غلظت 100 و 200 میکرومولار به‌مراتب بیش از دانهالهای شاهد بود. همچنین کاربرد برگی متیل‌جاسمونات محتوای آب نسبی و شاخص سرمازدگی برگ را در دانهالهای سرمادیده کاهش داد، ولی محتوای کلروفیل برگ را در مقایسه با دانهالهای شاهد افزایش داد. این تغییرات در راستای افزایش تحمل به سرما در دانهال‌ها صورت گرفت. محلولپاشی متیل‌جاسمونات به‌ویژه با غلظت 200 میکرومولار قادر به افزایش تحمل به سرما در دانهالهای خیار است و می‌تواند به‌عنوان یک ابزار پیشگیری‌کننده برای محافظت از سرمازدگی در گلخانه‌های خیار به‌کار رود.
کلیدواژه‌ها
عنوان مقاله [English]
Effect of foliar application of methyl jasmonate in cold tolerance improvement of greenhouse-grown cucumber cv. ‘Negin’ seedlings
نویسندگان [English]
- Shiva Bazl 1
- Rouholah Karimi 2
- Ahmad Ershadi 3
- Alireza Shahbodaghlo 4
- Mousa Rasouli 5
1 M.Sc., Department of Horticultural Science, Faculty of Agriculture, Buali Sina University, Hamedan, Iran
2 Assistant Professor, Department of Landscape, Faculty of Agriculture, Malayer University, Malayer, Iran
3 Associate Professor, Department of Horticulture, Faculty of Agriculture, Buali Sina University, Hamedan, Iran
4 . M.Sc., Department of Horticultural Science, Faculty of Agriculture, Buali Sina University, Hamedan, Iran
5 Assistant Professor, Department of Landscape, Faculty of Agriculture, Malayer University, Malayer, Iran
چکیده [English]
Cold stress is one of the limiting factors in production of greenhouse vegetables. Chemicals offer useful approach to reduce low temperature-induced damages. This study was conducted to evaluate the effect of foliar application of methyl jasmonate (MeJA) at concentrations of 0 (control), 50, 100, 200 µM) on electrolyte leakage (EL), relative water content (RWC), soluble carbohydrates, proline, chlorophyll content and chilling index of greenhouse- grown cucumber (Cucumis sativus L. cv ‘Negin’) in 4-6 leaf stage. MeJA was sprayed two times/day on cucumber seedling in a greenhouse of Bu-Ali Sina University, as a factorial based on completely randomized design with five replications per treatment, inlate- summer of 2013. Two days after spraying, seedlings were transferred from 25ºC to cold chamber and were subjected to chilling treatments of 15, 10 and 5 ºC for 4 hours. The EL of all plants increased with reducing the temperature. However, in MeJA-treated plants, especially in application of 200 µM, a lower leaf EL was observed. A clear increase in soluble carbohydrates and proline concentration was observed with decreasing temperature from 15 to 5ºC in all plants while in MeJA -treated seedlings, especially in application of 200 and 100µM, a greater amount of these osmolytes were observed in compared to control. Moreover, exogenous application of MeJA reduced RWC and visual damage of cold-stressed seedlings but increased chlorophyll content in compared to control plants. These changes correlated with the increment of cold tolerance in the cucumber plants. The results indicated that MeJA application especially at 200 µM, has the capacity to improve chilling tolerance of seedlings and can be used as a prophylactic tool to protect against chilling injury of cucumber cultivars in greenhouses
کلیدواژه‌ها [English]
- Cold stress
- chlorophyll content
- electrolyte leakage
- soluble carbohydrates
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