اثر گلایسین بتائین بر مقاومت به سرمازدگی گیاهچه‌های خیار

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، گروه علوم باغبانی، دانشکدۀ کشاورزی، دانشگاه ایلام، ایلام

2 استادیار گروه علوم باغبانی، دانشکدۀ کشاورزی، دانشگاه بوعلی سینا، همدان

چکیده

گلایسین بتائین یکی از محافظت‌کننده‏های اسمزی است که در پاسخ به تنش‏های غیرزیستی در گیاه تجمع می‏یابد. آزمایشی برای بررسی اثر گلایسین بتائین در غلظت‌های صفر، 10، 20 و 30 میلی‏مولار با دو روش کاربرد خیساندن بذری و محلول‌پاشی برگی بر مقاومت به سرمازدگی گیاهچه‏های خیار رقم ‘سوپر دامینوس’ انجام گرفت. پس از تیمار گلایسین بتائین، در مرحلة دو برگ حقیقی، گیاهچه‏ها تحت تنش سرمایی با دمای 3 درجة سانتی‌گراد، به‌مدت شش روز و هر روز به‌مدت شش ساعت قرار گرفتند. تیمار گلایسین بتائین به‌طور مؤثری سبب افزایش پارامتر‌های رشدی و افزایش مقاومت به تنش سرمایی گیاهچه‏های خیار شد. کاربرد گلایسین بتائین با افزایش محتوای کلروفیل، پرولین و فعالیت آنتی‌اکسیدانی کل و کاهش نشت یونی، مالون دی‌آلدئید و پراکسید هیدروژن سبب کاهش صدمۀ سرمازدگی گیاهچه‏ها شد. بیشترین مقاومت سرمایی در غلظت‌های 20 و 30 میلی‌مولار گلایسین بتائین با روش کاربرد بذری نسبت به کاربرد برگی به‌دست آمد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of glycine betaine on chilling tolerance of cucumber seedlings

نویسندگان [English]

  • Forough Saydpour 1
  • Mohammad Sayyari 2
  • Fardin Ghanbari 1
1 . M.Sc. Student, Department of Horticulture, College of Agriculture, University of Ilam, Ilam
2 Assistance Professor, Department of Horticulture, College of Agriculture, University of Bu-Ali Sina, Hamedan
چکیده [English]

Glycine betaine (GB) is one of the osmotic plant-protecting members that accumulate in response to abiotic stresses. An experiment was, therefore, conducted to test whether GB application at various concentrations (0, 10, 20 and 30 mM) through seed soaking or foliar spray methods would protect cucumber seedlings, subjected to chilling stress. At two true leave stages, after GB treatment, the seedlings were exposed to chilling six h/day at 3°C for 6 days. Results showed that GB pre-treatment improved growth parameters of seedlings subjected to chilling stress and provided significant protection against chilling stress compared to non-GB-treated seedlings. GB prevented chilling injuries with increasing chlorophyll, proline content and antioxidant activities and decreasing electrolyte leakage, malondialdehyde and hydrogen peroxide content. The highest cold tolerance was obtained with 20 and 30 mM GB application and seed soaking method provided better protection compared to foliar spray method.
 

کلیدواژه‌ها [English]

  • xidant activity
  • electrolyte leakage
  • hydrogen peroxide
  • Malondialdehyde
  • Proline content

 

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