نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، مؤسسة تحقیقات مرکبات کشور، رامسر، ایران

2 کارشناس‌ارشد گروه باغبانی، دانشکدة علوم کشاورزی دانشگاه زنجان، زنجان، ایران

3 دانشیار گروه باغبانی، دانشکدة علوم کشاورزی دانشگاه زنجان، زنجان، ایران

چکیده

بیماری تریستزا یکی از مهم‌ترین بیماری‌های ویروسی مرکبات است. گرمادرمانی یکی از روش‌های مدیریت این بیماری است که در این تحقیق استفاده شد. بدین منظور 36 اصله نهال پرتقال رقم ‘تامسون ناول’ در فیتوترون قرارگرفت. ابتدا، نهال‌ها از طریق پیوند، آلوده شدند. به‌منظور اطمینان از آلودگی گیاهان آزمون سرولوژیکی الایزا استفاده شد. نهال‌ها به‌مدت دو هفته در دمای 35 و 30 درجة سانتی‌گراد روز و شب پیش‌تیمار شدند و بعد یازده هفته در دمای40 و 30 درجة سانتی‌گراد روز و شب، به‌مدت دو هفته در دمای 42 و32 درجة سانتی‌گراد روز و شب و در نهایت به‌مدت ده روز در دمای 44 و 34 درجة سانتی‌گراد روز و شب قرارگرفتند. در تیمار آخر، دوازده عدد از نهال‌ها خشک شد و در شش عدد از گیاهان باقی‌مانده آلودگی مشاهده نشد. در سایر گیاهان زنده (هجده عدد) بیماری مشاهده شد. در نهایت، از آنجا که گرمادرمانی زمینة مناسبی را جهت کاهش غلظت ویروس فراهم کرد، می‌‌توان از آن در کنار پیوند نوک شاخساره (STG) و بالا بردن ضریب اطمینان از حذف ویروس استفاده کرد.

کلیدواژه‌ها

عنوان مقاله [English]

Using of Thermotherapy for Elimination of Tristisa virus in Thomson navel orange

نویسندگان [English]

  • malek ghasemi 1
  • mahsa hashemi sajadi 2
  • vali rabiei 3

1 Assistant Professor, Department of Seed and Plant Improvement, Iran Citrus Research Institute, Ramsar, Iran

2 M.Sc., Department of Horticulture, Faculty of Agricultural Science, University of Zanjan, Zanjan, Iran

3 Associate Professor, Department of Horticulture, Faculty of Agricultural Science, University of Zanjan, Zanjan, Iran

چکیده [English]

Thermotherapy is one of the tristeza management methods that was used in this study. Thus 36 ͺ ‘thomson navel orange’ scions on soure orange rootstocks were placed in temperature controlled chamber (TCC) to investigate the effect of thermotherapy on elimination of Tristeza virus. At first, the scions were inoculated by grafting of infected buds and after six months tested by ELISA to ensure contamination. Plants were placed in temperatures including: 40&30, 42&32, 44&340C for eleven weeks, two weeks and 10 days, respectively. In the last temperature, 12 plants dried and in 6 of the remaining plants, there was not verified any infected by ELISA, Direct Tissue Blot Immunoassay and indicator plant tests after three months from the last experiment.Termotherapy is considered as one of the healthy ways of citrus seedlings depending on plant species and viruses infecting alone or together with shoot tip grafting (STG) leads to the production of healthy seedlings. Healthy subjects express differences in the percentage of low initial concentration of virus. The difference in results between studies and other findings show the need and basis for selecting varieties tolerant to heat.

کلیدواژه‌ها [English]

  • ELISA
  • Immuno printing
  • Indicator plant test
  • Sanitation
  • Tristeza
1 . Arif M, Ibrahim M, Ahmad A and Hassan SH (2005) Elimination of citrus tristeza closterovirus from citrus bud-wood through thermotherapy. Pakistan Journal of Botany. 37(2): 423-430.
2. Ballester-Olmos JF, Pina JA, Carbonell E, Moreno P, Hermoso de Mendoza A, Cambra M and Navarro L (1993) Biological diversity of citrus tristeza virus (CTV) isolates in Spain. Plant Pathology. 42: 219-229.
3. Bar-Joseph M, Garnsey SM, Gonsalves D, Moscovitz M, Purcifull DE, Clark MF and Loebenstein G (1979) The use of enzyme-linked immuno sorbent assay for detection of citrus tristeza virus. Phytopathology. 69: 190-194.
4. Bar-Joseph M, Marcus R and Lee RF (1989) The continuous challenge of citrus tristeza virus control. Annual Review of Phytopathology. 27: 291-316.
5. Bar-Joseph M and Garnsey SM (1981) Enzyme-linked immune sorbent assay (ELISA): principles and applications for diagnosis of plant virus. In: K. Mara-moroschand K.F. Harris. Plant Discases and Vectors. Ecology and Epidemiology. Academic Press, New York. Pp. 35-59.
6. Bar-Joseph M and Lee RF (1989) Citrus Tristeza Virus. Description of Plant Viruses. AAB,Wellesbourne (GB). No. 353. Pp 123.
7. Calavan EC, Roistacher CN and Nauer EM (1972) Thermotherapy of citrus for inactivation of certain viruses. Plant Disease Report. 56: 976-978.
8. Cambra M, Camarasa E, Gorris MT, Garnsey SM, Gumpf DJ and Tsai MC (1993) Epitope diversity of citrus triteza virus (CTV) isolates in Spain. In: Proc. Conf. Int. Organ. Citrus Virology. 12th (Eds): P Moreno, JV Graca and LW Timmer. IOCV, Riverside, California. Pp. 33-38.
9. Cambra M, Gorris MT, Marroquin C, Roman MP, Olmos A, Martinez MC, Hermoso de Mendoza A, Lopez A and Navarro L (2000a) Incidence and epidemiology of citrus tristeza virus in the valencian community of Spain. Virus Research. 71: 85-95.
10. Desjardins PR, Wallace JM, Lange CT and Drake RJ (1957) The suppression of tristeza virus symptoms in Mexican lime seedlings by heat treatment. Plant Disease. Report. 41: 230-31.
11. Gonsalves D, Purcifull DE and Garnsey SM (1978) Purification and Serology of citrus tristeza virus. Phytopathology. 68: 553-559.
12. Grant TJ, Jones JW and Norman GG (1960) Present status of heat treatment of citrus viruses. Proceedings of the Florida State Horticultural Society. 72: 45-48.
13. Grant TJ (1967) Effect of heat treatments on tristeza and psorosis viruses in citrus. Plant Disease Report. 41: 232-234.
14. Karasev AV, Boyko VP, Gowda S, Nikolaeva, OV, Hilf ME, Koonin EV, Niblett CL, Cline K, Gump DJ, Lee RF, Garnsey SM and Dawson WO (1995) Complete sequence of the citrus tristeza virus RNA genome. Virology 208: 511-520.
15. Kassanis B (1957) The use of tissue culture to produce virus-free clones from infected potato varieties. Annals of Applied Biology. 45: 422-427.
16. Lee RF and Bar-Joseph M (2000) Tristeza. In: Compendium of Citrus Diseases, 2nd edn (Ed.Timmer LW, Garnsey SM and Graham JH), APS Press, St Paul (US). Pp 61-63.
17. Matthews REF (1993) Diagnosis of plant virus diseases. CRC Press, Boca Raton, Florida. Pp 374.
18. Quak F (1977) Meristem culture and virus-free plants. In: J. Reinert and Y.P.S. Bajaj (eds). Applied and fundamental aspects of plant cells, tissue, and organ culture. Springer, Berlin. Pp. 598-615.
19. Roistacher CN (1977) Elimination of citrus pathogens in propagative bud-wood. 1. Budwood selection, indexing and thermotherapy. Proceedings of the International Society of Citriculture. 3: 965-972.
20. Roistacher CN and Bar-Joseph M (1984) Transmission of tristeza seedling yellow tristeza virus by Aphis gossypii from sweet orange, grapefruit, and lemon to Mexican lime, grape fruit, and lemon. In: S.M. Garnsey and L. W. Timmer (Eds.), IOCV, Riverside, CA. Pp. 9-18.
21. Roistacher CN and Calavan EC (1974a). Heat tolerance of preconditioned citrus bud-wood for virus inactivation. In: Proceedings of 5th Conference of International Organization of Citrus Virology. W.C. Price. (Ed.) University of Florida Press, Gainesville. Pp. 256-261.
22. Roistacher CN and Calavan EC (1974b) Inactivation of five citrus viruses in plants held at warm glass house temperatures. Plant Disease Report. 58: 850-853.
23. Roistacher CN and Moreno P (1991) The worldwide threat from destructive isolates of citrus triteza virus-A review. In: Proceedings of the 11th Conference of IOCV. RH Bransky, RH Lee and LW Timmer (Eds.), Reverside, CA. pp. 7-19.
24. Sekiya ME, Lawrence SD, McCaffery M and Cline K (1991) Molecular cloning and nucleotide sequencing of coat protein gene of tristeza virus. General Virology. 72: 1013-1020.
25. Skaria M, Roistacher CN and Dagraca JV (1996) A virus-free citrus bud wood program for Texas. Proceeding of International Society of Citriculture. Pp. 366-368.
26. Stubbs LL (1963) A phytotron cabinet for heat-therapy studies with virus-infected plants. Common wealth Phytopathology News. 9(4): 49-52.
27. Vives MC, Rubio L, Lopez C, Navas-Castillo J, Albiach-Marti MR, Dawson WO, Guerri J, Flores R and Moreno P (1999) The complete genome sequence of the major component of a mild citrus tristeza virus isolate. General Virology. 80: 811-816.