استفاده از گرمادرمانی برای حذف ویروس تریستیزا در نهال‌های پرتقال ‘تامسون’

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار، مؤسسة تحقیقات مرکبات کشور، رامسر، ایران

2 کارشناس‌ارشد گروه باغبانی، دانشکدة علوم کشاورزی دانشگاه زنجان، زنجان، ایران

3 دانشیار گروه باغبانی، دانشکدة علوم کشاورزی دانشگاه زنجان، زنجان، ایران

چکیده

بیماری تریستزا یکی از مهم‌ترین بیماری‌های ویروسی مرکبات است. گرمادرمانی یکی از روش‌های مدیریت این بیماری است که در این تحقیق استفاده شد. بدین منظور 36 اصله نهال پرتقال رقم ‘تامسون ناول’ در فیتوترون قرارگرفت. ابتدا، نهال‌ها از طریق پیوند، آلوده شدند. به‌منظور اطمینان از آلودگی گیاهان آزمون سرولوژیکی الایزا استفاده شد. نهال‌ها به‌مدت دو هفته در دمای 35 و 30 درجة سانتی‌گراد روز و شب پیش‌تیمار شدند و بعد یازده هفته در دمای40 و 30 درجة سانتی‌گراد روز و شب، به‌مدت دو هفته در دمای 42 و32 درجة سانتی‌گراد روز و شب و در نهایت به‌مدت ده روز در دمای 44 و 34 درجة سانتی‌گراد روز و شب قرارگرفتند. در تیمار آخر، دوازده عدد از نهال‌ها خشک شد و در شش عدد از گیاهان باقی‌مانده آلودگی مشاهده نشد. در سایر گیاهان زنده (هجده عدد) بیماری مشاهده شد. در نهایت، از آنجا که گرمادرمانی زمینة مناسبی را جهت کاهش غلظت ویروس فراهم کرد، می‌‌توان از آن در کنار پیوند نوک شاخساره (STG) و بالا بردن ضریب اطمینان از حذف ویروس استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Using of Thermotherapy for Elimination of Tristisa virus in Thomson navel orange

نویسندگان [English]

  • malek ghasemi 1
  • mahsa hashemi sajadi 2
  • vali rabiei 3
1 Assistant Professor, Department of Seed and Plant Improvement, Iran Citrus Research Institute, Ramsar, Iran
2 M.Sc., Department of Horticulture, Faculty of Agricultural Science, University of Zanjan, Zanjan, Iran
3 Associate Professor, Department of Horticulture, Faculty of Agricultural Science, University of Zanjan, Zanjan, Iran
چکیده [English]

Thermotherapy is one of the tristeza management methods that was used in this study. Thus 36 ͺ ‘thomson navel orange’ scions on soure orange rootstocks were placed in temperature controlled chamber (TCC) to investigate the effect of thermotherapy on elimination of Tristeza virus. At first, the scions were inoculated by grafting of infected buds and after six months tested by ELISA to ensure contamination. Plants were placed in temperatures including: 40&30, 42&32, 44&340C for eleven weeks, two weeks and 10 days, respectively. In the last temperature, 12 plants dried and in 6 of the remaining plants, there was not verified any infected by ELISA, Direct Tissue Blot Immunoassay and indicator plant tests after three months from the last experiment.Termotherapy is considered as one of the healthy ways of citrus seedlings depending on plant species and viruses infecting alone or together with shoot tip grafting (STG) leads to the production of healthy seedlings. Healthy subjects express differences in the percentage of low initial concentration of virus. The difference in results between studies and other findings show the need and basis for selecting varieties tolerant to heat.

کلیدواژه‌ها [English]

  • ELISA
  • Immuno printing
  • Indicator plant test
  • Sanitation
  • Tristeza
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