نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی کارشناسی ارشد گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران
2 کارشناس ارشد گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران
3 دانشیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران
چکیده
سازوکار تحمل تنش خشکی پایة GF677، هیبرید هلو و بادام (Prunus persica × Prunus amygdalus) در شرایط درونشیشهای بررسی شد. گیاهچه‌های پایة GF677 به محیط کشت پرآوری جامد موراشیگ و اسکوگ (MS) حاوی 1 میلی‌گرم در لیتر بنزیل‌آدنین (BA) و 1/0 میلی‌گرم در لیتر نفتالین استیک اسید (NAA) در چهار سطح خشکی صفر (شاهد)، 10، 20 و 30 گرم بر لیتر پلی‌اتیلن گلیکول 6000 (به ترتیب، معادل پتانسیل اسمزی صفر، 2/0-، 4/0- و 6/0- مگاپاسکال) واکشت شد. بعد از گذشت شش هفته نتایج نشان داد تنش خشکی القاشده اثر معناداری بر پارامترهای اندازه‌گیری شده داشت. با افزایش سطوح خشکی در محیط کشت، فعالیت آنزیم‌های آنتی‌اکسیدانت (کاتالاز و پراکسیداز)، مقدار پروتئین کل و مقدار پرولین به طور معناداری افزایش یافت، در حالی که قندهای محلول افزایش غیرمعناداری در سطوح مختلف خشکی داشتند. از نتایج حاصل می‌توان چنین استنباط کرد که مهم‌ترین سازوکار تحمل به تنش خشکی پایة GF677 در شرایط درونشیشهای، به‌کارگیری سیستم دفاع آنتی‌اکسیدانتی، افزایش سنتز پروتئین (افزایش بیان ژن‌ها) و تجمع پرولین است. تنظیم اسمزی با قندهای محلول اهمیت کمتری دارد.
کلیدواژه‌ها
عنوان مقاله [English]
Mechanism of drought stress tolerance of GF677 rootstock (peach and almond hybride) under in vitro conditions
نویسندگان [English]
- Mehri Mashayekhi 1
- Fariborz Habibi 2
- Mohamad Amiri 3
1 M.Sc. Student, Department of Horticultural Science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
2 M.Sc., Department of Horticultural Science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
3 Associate Professor, Department of Horticultural Science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
چکیده [English]
Mechanisms of drought stress tolerance of GF677 rootstock, peach and almond hybrid, (Prunus persica × Prunus amygdalus) were studied under in vitro conditions. Plantlets of GF677 rootstock were subcultured into the Murashige and Skoog (MS) solid proliferation medium containing 1 mg/l BA (6-Benzyladenine) and 0.1 mg/l NAA (naphthaline acetic acid) in four drought stress levels 0 (control), 10, 20 and 30 g/l polyethylene glycol 6000 (corresponding to osmotic potentials 0, -0.2, -0.4 and -0.6 Mpa, respectively). After six weeks results showed that induced drought stress had a significant effect on measured parameters. By increasing drought levels in the culture medium, antioxidant enzymes activity (catalase and peroxidase), total protein content and proline content significantly increased; meanwhile soluble sugars had non-significant increasing in the different levels of drought. According to the results it can be concluded that, the most important mechanisms of drought tolerance of GF677 rootstock under in vitro conditions are the use of antioxidant defense system, increasing protein synthesis (enhancing genes expression) and proline accumulation and osmoregulation by soluble sugars had less important.
کلیدواژه‌ها [English]
- Antioxidant Enzymes
- Osmoregulation
- proline
- soluble sugars
- total protein
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