نوع مقاله : مقاله پژوهشی

نویسنده

استادیار، بخش زراعت، مرکز تحقیقات کشاورزی و منابع طبیعی استان یزد، یزد – ایران

چکیده

یکی از تأثیرات کاهش آب در خاک، کاهش رشد و نمو گیاهچه‌های سبزشده و تغییر در نمو مزرعه‌ای آنها در چنین شرایطی است. مشخص شده که پیش‌تیمار بذرها موجب بهبود برخی خصوصیات گیاهچه و گیاه حاصل می‌شود. به‌منظور بررسی اثر پیش‌تیمار بذر جو با سالیسیلیک اسید بر رشد گیاهچۀ جو، مقدار پرولین و تغییر فعالیت آنزیم‌های آنتی‌اکسیدانتی تحت شرایط تنش خشکی آزمایشی در سال 1391 در مرکز تحقیقات کشاورزی و منابع طبیعی استان یزد به اجرا درآمد. فاکتور اول شامل غلظت‌های مختلف سالیسیلیک اسید (صفر، 50، 100 و 150 قسمت در میلیون) و فاکتور دوم شامل سه سطح خشکی (صفر، 6- و 12- بار) بود. نتایج نشان داد که اثر سالیسیلیک اسید و تنش خشکی بر درصد جوانه‌زنی، سرعت جوانه‌زنی، وزن خشک گیاهچه، پروتئین، کاتالاز، آسکوربات پراکسیداز و پرولین معنا‌دار بود. اثر متقابل تنش و تیمار بذر بر تعداد برگ معنادار نبود، ولی تأثیرات اصلی آنها معنادار بود. بیشترین درصد جوانه‌زنی (7/98 درصد)، سرعت جوانه‌زنی و وزن خشک گیاهچه مربوط به غلظت 100 قسمت در میلیون سالیسیلیک اسید بود. تحت شرایط تنش خشکی، پروتئین کاهش یافت که استفاده از سالیسیلیک اسید مقدار آن را افزایش داد. همچنین تحت شرایط تنش فعالیت کاتالاز، آسکوربات پراکسیداز و پرولین افزایش یافت که استفاده از سالیسیلیک اسید سبب کاهش در آنها شد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of salicylic acid pretreatment of barley seed on seedling growth, proline content and antioxidant enzymes activity under drought stress

نویسنده [English]

  • sayed ali tabatabaei

Assistant Profossor, Department of Agronomy, Agriculturale and Natural Resources Research Center of Yazd, Yazd, Iran

چکیده [English]

One of the effects of reducing water content on soil is reduction of growth and development of seedlings and variation of field development of them. It was found that seed priming improves some characteristics of seedling and plant. An experiment was conducted for evaluating the effect of seed priming by salicylic acid on some characteristics of barley seed at early growth stages, antioxidant enzyes activity and proline content under drought stress. The first factor was priming with salicylic acid (zero, 50, 100 and 150 ppm) and the second factor was three levels of drought (zero, -6 and -12 bar). The results showed that SA pretreatment effect on germination percentage, germination rate, dry weight, protein, catalase, ascorbat peroxidase activity and proline content were significant. The interaction effect of stress  seed treatment on leaf number was not significant but the main factors were significant. The highest germination percentage (98.7), germination rate and seedling dry weight were found at 100 ppm concentrations of salicylic acid. Under drought stress conditions, protein content decreased but salicylic acid pretreatment increased the valuet. Also, drought stress conditions increased enzyme activity and proline content, but salicylic acid pretreatment decreased them.

کلیدواژه‌ها [English]

  • Barley
  • Drought stress
  • enzyme
  • proline
  • Salicylic Acid
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