اثر محیط کشت و تنظیم‌کننده‌های رشد بر ریزازدیادی پایه گزیلا 6

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس ارشد باغبانی، گروه باغبانی، دانشکدۀ کشاورزی، دانشگاه آزاد اسلامی واحد شیروان، شیروان، ایران

2 استادیار، گروه باغبانی، مرکز تحقیقات کشاورزی و منابع طبیعی خراسان رضوی، مشهد، ایران

3 استادیار، بخش تحقیقات اصلاح و تهیۀ نهال و بذر، مرکز تحقیقات کشاورزی و منابع طبیعی خراسان رضوی، مشهد، ایران

چکیده

این مطالعه با هدف بررسی اثر محیط کشت و تنظیم‌کننده‌های رشد گیاهی بر سرعت پرآوری، ریشه‌زایی و سازگاری پایۀ گزیلا 6 طی سال 1390-91، در مرکز تحقیقات کشاورزی و منابع طبیعی خراسان رضوی انجام گرفت. از شش نوع محیط کشت شامل MS، WPM و DKW در دو حالت جامد و مایع (محیط مایع به‌همراه پرلیت) و تنظیم‌کننده‌های رشد TDZ و BAP در غلظت‌های صفر، یک و دو میلی‌گرم در لیتر به‌همراه 1/0 میلی‌گرم در لیتر IBA در مرحلۀ پرآوری و محیط‌های ذکرشده به‌همراهIBA  در غلظت‌های صفر، یک، دو و سه میلی‌گرم در لیتر، در مرحلۀ ریشه‌زایی استفاده شد. نتایج نشان داد بیشترین تعداد و طول شاخه به‌ترتیب به‌مقدار 48/6 شاخه و 14/3 سانتی‌متر در محیط WPM جامد دارای دو میلی‌گرم در لیتر BAP و محیط  MSمایع با یک میلی‌گرم در لیتر TDZ و بیشترین درصد ریشه‌زایی و طول ریشه به‌مقدار 90/93 درصد و 76/11 سانتی‌متر، در محیط MS مایع دارای دو میـلی‌گرم در لـیتر IBA بـه‌دست آمد. با اینکه کاربرد محیط‌های کشت مایع در میانگین کلی به سازگاری بهتر گیاهچه‌ها منجر شد، بیشترین درصد بقای گیاهچه (80/91 درصد) در محیط  WPMجامد با دو میلی‌گرم در لیتر IBA به‌دست آمد.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of culture media and growth regulators on micropropagation of Gisela 6 rootstock

نویسندگان [English]

  • zeinab karamad 1
  • Ebrahim Ganji Moghadam 2
  • ahmadreza bolandi 3
1 M.Sc. Student, Department of Horticulture, Islamic Azad University, Shirvan Branch, Islamic Azad University, Shirvan, Iran
2 Assistant Professor, Department of Horticulture, Khorasan Razavi Natural Resource and Agricultural Research Center, Mashhad, Iran
3 Assistant Professor, Department of Seed and Plant Improvement of Khorasan Razavi Natural Resource and Agricultural Research Center, Mashhad, Iran
چکیده [English]

This study was conducted with the main prupose of investigation the effects of culture medium and the concentration of growth regulators on proliferation rate, rooting and the acclimatization of Gisela 6 rootstock at Khorasan Razavi Agriculture and Natural Resource Research Center during 2011-2012. In this experiment, the proliferation and rooting were performed in six kinds of culture medium (DKW, WPM and MS in two solid and liquid media) supplemented with plant growth regulators included TDZ and BAP in zero, one, two mg/l plus 0.01 mg/l IBA in all treatments of the proliferation and IBA with zero, one, two, three mg/l in rooting step. The results showed that the highest number and length of shoot were 6.48 shoot and 3.14 cm, respectively in solid WPM medium and two mg/l BAP concentration and liquid MS medium with one mg/l concentration of TDZ. The liquid MS medium in two mg/l of IBA caused the highest percentage of rooting and root length, 93.90 percent in 11.76 cm, respectively. Although applying the liquid media resulted in better acclimatization of plantlets, but the highest percent of plantlets survival was obtained in WPM liquid medium with two mg/l concentration of IBA at about 91.80 percent.

کلیدواژه‌ها [English]

  • Acclimatization
  • Cherry
  • proliferation
  • rooting
  • Rootstock
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