تأثیر کاربرد خارجی پرولین و گلایسین‌بتائین بر تغییرات بیوشیمیایی در انگور در وضعیت تنش خشکی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه علوم باغبانی، دانشکدۀ کشاورزی، دانشگاه زنجان، زنجان - ایران

2 دانشیار، گروه علوم باغبانی، دانشکدۀ کشاورزی، دانشگاه زنجان، زنجان - ایران

3 دانشیار، بخش تحقیقات اصلاح و تهیۀ نهال و بذر، مرکز تحقیقات کشاورزی و منابع طبیعی قزوین، قزوین - ایران

4 استادیار، بخش خاک و آب، مرکز تحقیقات کشاورزی و منابع طبیعی زنجان، زنجان - ایران

چکیده

پرولین و گلایسین‌بتائین از رایج‌ترین مواد آلی سازگارند که با تنظیم اسمزی و محافظت از غشا‌ها، پروتئین‌ها و آنزیم‌ها از تأثیرات مخرب تنش‌های اسمزی در گیاهان جلوگیری می‌کنند. براساس گزارش‌ها، با کاربرد خارجی این ترکیبات در گیاهان، تحمل آنها به خشکی را می‌توان افزایش داد. بدین­منظور، این تحقیق به‌صورت فاکتوریل بر پایۀ طرح بلوک‌های کامل تصادفی در چهار تکرار بر روی چهار رقم انگور ʼخوشناوʻ، ʼپیکانیʻ، ʼپرلتʻ وʼفلیم سیدلسʻ اجرا شد. تاک‌های تحت تنش خشکی با 70 درصد از آب مورد نیاز انگور آبیاری شدند. پرولین (10 میلی‌مولار) و گلایسین‌‌بتائین (15 میلی‌مولار) در چهار مرحله (پیش از گلدهی، گلدهی، غوره شدن و رنگ گرفتن خوشه‌های انگور) روی تاک‌ها محلول‌پاشی شد. نتایج نشان داد که مقدار پرولین و گلایسین‌بتائین درونی گیاه و فعالیت آنزیم‌های پراکسیداز و آسکوربات‌پراکسیداز در هر دو تیمار پرولین و گلایسین‌بتائین به‌طور معنا‌داری بیشتر از شاهد بود. بیشترین و کمترین مقدار پرولین به‌ترتیب در ارقام ʼفلیم سیدلسʻ و ʼپیکانیʻ ارزیابی شد، اما دیگر صفات اندازه‌گیری‌شده اختلاف معنا‌داری در بین ارقام نشان ندادند. با توجه به نقش این آنزیم‌ها در از بین بردن گونه‌های فعال اکسیژن و همچنین نقش پرولین و گلایسین‌بتائین در فعال کردن آنتی‌اکسیدان‌ها، با کاربرد این دو اسمولیت می‌توان تحمل انگور را در مقابل تنش خشکی افزایش داد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of exogenous application of proline and glycine betaine on biochemical alterations in grapevine under drought stress

نویسندگان [English]

  • mahdi mohammad zan=mani 1
  • vali rabiei 2
  • Mohammad Ali Nejatian 3
  • Mehdi Taheri 4
1 M.Sc. Student, Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
2 Associate Professor, Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
3 Associate Professor, Department of Seed and Plant improvement, Agriculture and Natural Resources Research Center of Qazvin, Qazvin, Iran
4 Assistant Professor, Department of Soil and Water, Agriculture and Natural Resources Research Center of Zanjan, Zanjan, Iran
چکیده [English]

Proline and glycine betaine are the most common compatible solutes that prevent plants from destructive effects of osmotic stresses by osmotic adjustment and protection of membranes, proteins and enzymes. It has been reported that exogenous application of these compounds, can increase plant resistance to drought stress. Therefore a factorial experiment based on randomized complete block design with four replications was performed in four grapevine cultivars (‘Khushnav’, ‘Peykani’, ‘Perlette’, and ‘Flame Seedless’). Grapevines were irrigated by 70 percent of the vine water requirement, in drought stress treatment. Proline (10 mM) and glycine betaine (15 mM) were sprayed on grapevines at four growth stages (before flowering, flowering, sour cluster and veraison). Results showed that endogenous proline and amount of glycine betaine, peroxidase and ascorbate peroxidase enzyme activity in treated vines were significantly higher than the control, in both proline and glycine betaine treatments. Among of the treated vines, the most and the least amount of proline was measured in ‘Flame Seedless’ and ‘Peykani’, respectively. Other measured traits in cultivars did not show significant difference. According to the role of these enzymes in eliminating reactive oxygen spices (ROS) and also the role of proline and glycine betaine in antioxidants activating, using of these two osmolytes will be able to increase the vines tolerance to drought stress conditions.

کلیدواژه‌ها [English]

  • Antioxidant
  • Ascorbate peroxidase
  • Osmoregulation
  • Peroxidase
  • water stress
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