کاربرد باکتری های محرک رشد بر خصوصیات جوانه زنی بذور دو رقم یونجه تحت شرایط تنش شوری

نویسندگان

1 دانشجوی دکتری، گروه زراعت و اصلاح نباتات، دانشکده علوم و مهندسی زراعی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج

2 استاد، گروه زراعت و اصلاح نباتات، دانشکده دانشکده علوم و مهندسی زراعی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج

3 دانشیار، گروه زراعت و اصلاح نباتات، دانشکده دانشکده علوم و مهندسی زراعی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج

4 استادیار، گروه مهندسی علوم خاک، دانشکده مهندسی و فناوری کشاورزی، دانشگاه تهران، کرج

چکیده

به منظور ارزیابی تأثیر کاربرد باکتری های افزاینده رشد گیاه بر جوانه زنی و رشد اولیه یونجه در شرایط تنش شوری، آزمایشی در آزمایشگاه تحقیقات بذر دانشکده علوم و مهندسی زراعی پردیس کشاورزی و منابع طبیعی، دانشگاه تهران به صورت فاکتوریل در قالب طرح کاملاً تصادفی اجرا گردید. تیمارهای آزمایشی شامل سه سطح تنش شوری شامل شاهد (بدون تنش) (S0)، 60 (S1) و 120 (S2) میلی مولار نمک کلریدسدیم، دو رقم یونجه ‘بمی’ و ‘یزدی’ و 16 پیش تیمار مختلف باکتریایی بود. سطوح پیش تیمار باکتریایی شامل شاهد (بدون باکتری)، ازتوباکتر، آزوسپریلیوم، سودوموناس و ریزوبیوم ملیلوتی به صورت منفرد و نیز به صورت تلفیق دوتایی، سه تایی و چهارتایی از باکتریهای مختلف بود که در مجموع 16 سطح پیش تیمار مختلف باکتریایی را تشکیل می داد. اعمال تنش شوری منجر به کاهش معنی دار جوانه زنی و رشد اولیه گیاهچه گردید که این کاهش در تیمار شاهد (عدم پیش تیمار بذر) در بیشترین مقدار خود مشاهده شد. به کارگیری پیش تیمار باکتریایی به ویژه تیمار باکتری سودوموناس و تیمارهای تلفیقی نقش مؤثری در تعدیل اثرات منفی شوری بر صفات مورد ارزیابی داشتند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Growth Promoting Rhizobacteria on germination and early growth of two alfalfa cultivars under salinity stress condition

نویسندگان [English]

  • Omid Younesi 1
  • Kazem Poustini 2
  • Mohammad Reza Chaichi 3
  • Ahmad Ali Pourbabaie 4
چکیده [English]

In order to study the effects of seed-bacterial priming (inoculation) on germination and early growth of alfalfa under salinity stress conditions, an experiment was conducted at seed research laboratory and Greenhouse of College of Agriculture, University of Tehran in Karaj (Iran) in 2011. The experiment was arranged as a factorial in Completely Randomized Design (CRD) with three replications. Experimental treatments including: three levels of salinity stress (zero (S0), 60 (S1) and 120 (S2) mmol), two levels of alfalfa cultivars (‘Bami’ and ‘Yazdi’) and 16 levels of bacterial priming (Azetobacter, Azospirillum, Pseudomonas and Rhizobium meliloti in single and different double, triple and quadratic integrated forms). The results indicated that applying salinity stress significantly decreased germination and early seedling growth. This descending trend in control (no inoculation) treatment was more than that of treated seeds. Application of bacterial priming especially Pseudomonas priming and integrated treatments played an important role in moderating the negative effects of salinity on measured traits. According to the results of this study, it seems that plant growth promoting bacteria, by producing and releasing phytohormones such as auxin, gibberellins and cytokinin along with decreasing ethylene level, improve plant growth under salinity stress condition.

کلیدواژه‌ها [English]

  • Alfalfa
  • Early growth
  • germination
  • Growth Promoting Rhizobacteria (GPR)
  • salinity

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