اثر صفات روزنه ای بر فتوسنتز و عملکرد لینه های جایگزین کروموزومی گندم نان در شرایط طبیعی و تنش خشکی

نویسندگان

1 مربی، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه آزاد اسلامی واحد نراق، نراق

2 دانشیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه شهرکرد، شهرکرد

3 استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه شهرکرد، شهرکرد

چکیده

این مطالعه به منظور تعیین اثرات اندازه و فراوانی روزنه بر سرعت فتوسنتز، هدایت روزنه ای، عملکرد و همچنین تعیین مکان کروموزومی ژن های کنترل کننده این خصوصیات با استفاده از لاین های جایگزین کروموزومی رقم تایمستین در زمینه ژنتیکی رقم چاینیز اسپرینگ در قالب طرح بلوک های کامل تصادفی با سه تکرار در دو شرایط نرمال و تنش آب در مزرعه تحقیقاتی دانشکده کشاورزی، دانشگاه شهرکرد در سال 1387 انجام شد. شرایط مرحله طویل شدن ساقه (مرحله 29 زادکس) شروع شد. طول مدت تنش براساس درجه روز رشد مشخص شده در آزمایشات قبلی تعیین شد. تفاوت های معنی داری بین لاین های جایگزین در خصوص صفات مورد بررسی مشاهده شد. در شرایط بدون تنش، همبستگی عملکرد با فراوانی روزنه (**461/0 = r) و در شرایط تنش، همبستگی عملکرد و اندازه روزنه (**450/0 = r) معنی دار بود. همچنین، همبستگی عملکرد با سرعت فتوسنتز (**556/0 =r در شرایط تنش و **482/0 =r در شرایط بدون تنش) و هدایت روزنه ای (**247/0 =r در شرایط تنش و **457/0 =r در شرایط بدون تنش) هم در شرایط تنش و هم در شرایط بدون تنش معنی دار بود. بررسی کروموزوم‌های گندم نشان داد نقش ژنوم B نسبت به دو گروه دیگر در کنترل صفات مورد بررسی برجسته تر بود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of stomatal characteristics on photosynthesis and yield of the bread wheat chromosomal substitution lines under normal and stress conditions

نویسندگان [English]

  • Roghayeh Aminian 1
  • Shahram Mohammadi deh cheshme 2
  • Saadollah Houshmand 3
  • Mahmoud Khodambashi 2
  • Karim Nozad 1
چکیده [English]

This study was conducted to determine the effects of stomatal size and frequency on stomatal conductance, photosynthesis rate and yield, and to locate the genes controlling these traits. Therefore, substitution lines series of ‘Timstein’ (Tim) into genetic background of ‘Chinese Spring’ (CS) were tested in a completely randomized block design with three replications under two normal and water stress conditions in Shahrekord University research farm. Stress condition was started at elongation stage and irrigation periods were scheduled based on pre-determined ‘Growth Degree Day’ (GDD) during the length of stress period. Significant differences were found among substitution lines in terms of stomatal frequency, stomatal size, stomatal conductance, photosynthetic rate and yield. The significant correlation was found between the yield with stomatal size (r = 0.450**) and stomatal frequency (r = 0.461**) in stress and non-stress condition, respectively. In addition, there were significant correlation between yield with photosynthetic rate (r = 0.556** in stress condition and r = 0.482** in non-stress condition) and stomatal conductance (r = 0.247** in stress condition and r = 0.457** in non-stress condition). Based on the obtained results, it can be concluded that role of group B chromosomes was more prominent than the other two groups.

کلیدواژه‌ها [English]

  • Photosynthetic rate
  • Stomatal conductance
  • Stomatal Frequency
  • Stomatal size
  • Substitution lines

1 . امینیان ر (1389)  مطالعات ژنومی عملکرد و اجزای آن و صفات مرتبط با تحمل خشکی در گندم نان. پایان­نامه دکتری اصلاح نباتات دانشگاه شهرکرد. شهرکرد.

2 . عبدمیشانی س. و شاه نجات بوشهری ع ا (1376) اصلاح نباتات  تکمیلی. جلد اول. انتشارات دانشگاه تهران.

3 . فرشادفر ع. و محمدی ر (1384) تجزیه کنترل ژنتیکی تحمل به خشکی در گندم رقم شاین با استفاده از لاین­های جایگزین. نهال و بذر 21(­1):  108-93. 

 

4 . Aryavand A, Ehdaie B, Tran B and Waines JG (2003) Stomatal frequency and size differentiate ploidy levels in Aegilops neglecta. Genetic Resources and Crop Evolotion 50: 175-182

5 . Bhagwat SG and Bhatia CR (1993) Selection for flag leaf stomatal frequency in bread wheat. Plant Breeding 110: 129-136.

6 .  Ciha AJ and Brown WA (1975) Stomatal size and frequency in soybean. Crop Science 15: 309-313.

7 . Cowan IR (1986) Economics of carbon fixation in higher plants, In: Givnish, T. J., (ed) On the economy of plant form and function, Cambridge university press, Combidge, UK. pp. 133-170.

8 . Condon AG, Richards RA, Rebetzek GJ and Farquhar GD (2004) Breeding for high water use efficiency. Experimental Botonty 55: 2447-2460.

9 . Ewers BE, Oren R, Johnsen KH and Landsberg JJ (2001) Estimating maximum mean canopy stomatal conductance for use in models. Canadian Journal of Forest Resources 31: 198-207.

10 . Farquhar GD, Buckley TN and Miller JM (2002) Optimal stomatal control in relation to leaf area and nitrogen content. Silva Fennica 36(3): 625-637.

11 . Heichel GH (1971) Genetic control of epidermal cell and stomatal frequency in maize. Crop Science 11: 830-832.

12 . Hetherington AM and Woodward FI (2003) The role of stomata in sensing and driving environmental change. Nature 242: 901-908.

13 . Hyeon-Hye K, Gregory DG, Raymond MW and John CS (2004) Stomatal conductance of lettuce grown under or exposed to different light qualities. Annales of Botony 94: 691-967.

14 . Izhar S and Wallace DH (1967) Genetic variation in photosynthetic efficiency of Phaseolus vulgaris. Crop Sci.
7: 457-460.

15 . Janice L, Cuthbert D, Somers J, Anita L, Brule-Babel P, Douglas B and Gary H (2008) Molecular mapping of quantitative trait loci for yield and yield components in spring wheat (Triticum aestivum L.) Theorerical Applied Genetics 17: 595-608.

16 . Jarvis PG (1976) The interpretation of variations in leaf water potential and stomatal conductance found in canopies in the field. Philos. Trans. R. Soc. London. 273: 93-610.

17 . Jianwu T, Paul VB, Brent EE, Ankur RD and Kenneth JD (2006) Sap-flux-upscaled canopy transpiration, stomatal conductance and water use efficiency in an old growth forest the GreatLakes region of the United States. Journal of Geophysical Research-Biogeosciences Vol. 111.

18 . Khazaei H, Monneveux P, Shao H and Mohammady SH (2010) Variation for stomatal characteristics and water use efficiency among diploid, tetraploid and hexaploid Iranian wheat landraces. Genetic Resoures and Crop Evolution 57: 307-314.

19 . Ma R, Kondo M, Ideta O, Barlaan E and Imbe T (2010) Quantitative trait loci for stomatal density and size in lowland rice. Euphytica 172: 149-158.

20 . Mackay DS, Ahl DE, Ewers BE, Samanta S, Gower ST and Burrows SN (2003) Physiological tradeoffs in the parameterization of a model of canopy transpiration. Advances in Water Resources 26: 179-194.

21 . Maghsoudi K and Maghsoudi moud A (2008)Analysis of the Effects of Stomatal Frequency and Size on Transpiration and Yield of Wheat (Triticum aestivum L.). American Eurasian Journal of Agricultural and Environmental Sciences 3(6): 865-872.

22 . Merah O, Monneveux P and Dele´ens E (2001) Relationship between flag leaf carbon isotope discrimination and several morpho-physiological traits in durum wheat genotypes under Mediterranean conditions. Environmental Experimental Botony 45: 63-71.

23 . Miskin KE, Rasmusson DC and Moss DN (1972) Inheritance and physiological effects of stomatal frequency in barley. Crop Sciences 12: 780-783.

24 . Mohammady S (2002) Inheritance of tolerance to water stress in wheat (Triticum aestivum L.). Ph.D. Thesis. University of Newcastle, UK.

25 .  Pushpendra KG, Harindra SB, Pawan LK, Neeraj K, Reyazul RM,  Amita M and Jitendra K (2007) QTL analysis for some quantitative traits in bread wheat. Zhejiang University Science 8(11): 807-814.

26 . Rajendra BR, Mujeeb KA and Bates LS (1978)
Relationships between 2x Hordum sp., 2x Secale sp. and 2x, 4x, 6x Triticum sp. for stomatal frequency, size and distribution. Environmental and Experimental Botony 18: 33-37.

27 . Raschke K (1975) Stomatal action. Annual Review of Plant Physiology 26: 309-340.

28 . Shimshi D and Ephrat J (1975) Stomatal behavior of wheat cultivars in relation to their transpiration photosynthesis and yield. Agron. 67: 326-331.

29 . Singh S and Sethi GS (1995) Stomatal size, frequency and distribution in Triticum aestivum, Secale cereale and their amphiploids. Cereal Research Communication 23: 103-108

30 . Sishen L, Jizeng J, Xianyun W, Xiaocun Z, Linzhi L, Haimei C, Yuding  F, Haiyan S, Xinhua  Z  and Tiandong L (2007) A intervarietal genetic map and QTL analysis for yield traits in wheat. Molecular Breeding 20: 167-178.

31 . Teare ID, Peterson CJ and Law AG (1971) Size and frequency of leaf stomata in cultivars of Triticum aestivum and other Triticum species. Crop Sciences 11: 496-498.

32 . Venora G and Calcagno F (1991) Study of stomatal parameters for selection of drought resistant varieties in Triticum durum DESF. Euphytica 57: 275-283.

33 . Wang H and Clarke JM (1993a) Genotypic, intra plant and environmental variating in stomatal frequency and size in wheat. Canadian Journal of Plant Science 73: 671-678.

34 . Wang H and Clarke JM (1993b) Relationship of excised-leaf water-loss and stomatal frequency in wheat. Canadian Journal of Plant Sciences 73: 93-99.

35 . Zadoks JC, Change TT and Knozak CF (1974) A decimal code for growth stages of cereals. Weed Research 14: 415-421.

36 . Zhang ZB, Shao HB, Xu P, Chu L, Lu Z and Tian J (2007) On evolution and perspectives of bio-water saving. Colloids. Surf. B. Biointerfaces 55: 1-9.