اثر تنش شوری بر واکنش‌های فیزیولوژیکی گیاهچه‌های چهار پایه مرکبات در شرایط درون شیشه‌ای

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد، گروه مهندسی باغبانی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

2 دانشیار، گروه مهندسی باغبانی، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

چکیده

شوری یکی از مشکلات تولید مرکبات در جهان است و تحمل یا مقاومت پایه‌های مرکبات ‌به شوری به خوبی شناخته نشده است. در این پژوهش، واکنش‌های فیزیولوژیکی چهار پایه نارنج (Citrus aurantium. L)، پونسیروس (Poncirus trifoliate Raf.)، سیتروملو (Citrumelo) و سیترنج (Citranges) به تنش شوری در آزمایشی به صورت فاکتوریل در قالب طرح کاملا تصادفی در شرایط درون شیشه‌ای در دانشگاه علوم کشاورزی و منابع طبیعی ساری بررسی شد. ریزنمونه‌های تهیه شده از هر چهار پایه به محیط کشت جامد موراشیگ و اسکوگ (MS) حاوی ۹/۸ میکرومولار BA و نیم میکرومولار NAA با غلظت‌های مختلف کلریدسدیم (صفر، ۵۰، ۱۰۰ و ۲۰۰ میلی‌مولار) در سه تکرار منتقل شدند. براساس نتایج به دست آمده در پایان آزمایش، وزن تر و خشک، محتوای آب‌نسبی، نشت‌یونی و میزان رنگیزه‌های فتوسنتزی از قبیل کلروفیل ‌a، کلروفیل ‌b و کارتنوئید گیاهچه‌ها دارای همبستگی منفی با غلظت تیمار شوری و غلظت یون سدیم در بافت برگ‌ها بود. اگر چه نشت‌یونی با افزایش غلظت کلریدسدیم در هر چهار پایه افزایش یافت ولی، پایه نارنج نسبت به سایر پایه‌ها از نظر آماری کمترین نشت‌یونی را نشان داد. در همه تیمارهای شوری میزان رنگیزه‌های فتوسنتزی پایه‌ها، کاهش معنی‌داری در سطح یک‌درصد نسبت به شاهد داشتند. در بین پایه‌های مورد آزمایش، میزان رنگیزه‌های فتوسنتزی و آب‌نسبی سیترنج بیشتر از دیگر پایه‌ها بود. در مجموع، پایه‌های سیترنج و نارنج مقاومت بهتری به آسیب‌های ناشی از تنش شوری نشان دادند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of salt stress on physiological responses of four citrus rootstock plantlets under in vitro condition

نویسندگان [English]

  • lale rostamian 1
  • Vida Chalavi 2
  • Hossein Sadeghi 2
1 Former M.Sc. Student, Department of Horticulture, Faculty of Agricultural Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2 Associate Professor, Department of Horticulture, Faculty of Agricultural Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
چکیده [English]

Salinity is one of citrus production problems in the world, and the tolerance or resistance mechanism of citrus rootstocks to salinity is not well known. In this study, the physiological responses of four citrus rootstocks, Sour orange (Citrus aurantium.L), Poncirus (Poncirus trifoliate Raf), Citromelo (Citrumelo) and Citrange (Citranges), to salinity stress was investigated in a factorial experiment based on completely randomized design under in vitro conditions. The explants were prepared from all four rootstocks and transferred in a Murashige and Skoog (MS) solid culture medium containing 8.9 μM BA and 0.5 μM NAA with different concentrations of sodium chloride (0, 50, 100 and 200 mM) in three replications. Based on obtained results of experiment, plantlets fresh and dry weight, water content, leakage and photosynthetic pigments such as chlorophyll a, chlorophyll b and carotenoid had a negative correlation with salinity concentration and concentration of sodium ion in the tissue of the leaves. Although leakage increased with increasing concentration of sodium chloride on all four rootstocks, the Sour orange rootstock statistically showed the least leakage as compared to other rootstocks. In all salinity treatments, the amount of rootstocks photosynthetic pigment reductions was statistically significant (p ≤ 0.01) as compared to the control. Among the tested rootstocks, the amount of photosynthetic pigments in the Citrange rootstock was higher than other rootstocks. In conclusion, Citrange and Sour orange rootstocks showed better resistance to the damages caused by salt stress.

کلیدواژه‌ها [English]

  • "Ion Leakage"
  • "Lipid Peroxidation"
  • "Relative water content"
  • "Sodium Chloride"
  • "Tissue culture"

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