نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه مهندسی علوم باغبانی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان - ایران

2 دانشیار گروه مهندسی علوم باغبانی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان – ایران

3 استادیار گروه مهندسی علوم باغبانی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان - ایران

چکیده

به منظور بررسی تأثیر پیش‏تیمار‏‏ بذری و محلول‏پاشی 24-اپی براسینولید بر برخی ویژگی‏های مورفولوژیک، فیزیولوژیکی و بیوشیمیایی گیاه همیشه ‏بهار آزمایشی به صورت فاکتوریل در قالب طرح کاملاً ‏تصادفی با سه تکرار در گلخانه تحقیقاتی دانشگاه شهید باهنر کرمان، در سال 1393 انجام گرفت. تیمارهای آزمایش شامل پیش‏تیمار‏ بذری و محلول‏پاشی 24-اپی براسینولید با چهار غلظت (0، 1/0، 1 و 10  میکرومولار) بود. نتایج نشان داد که خصوصیات رشدی و رنگیزه‏های فتوسنتزی با کاربرد این تنظیم‌کننده رشد به صورت پیش‏تیمار و محلول‏پاشی افزایش یافت. بیشترین وزن تر اندام هوایی، ریشه، وزن تر و خشک گل در تیمار ترکیبی (پیش‏تیمار 1/0 میکرومولار + محلول‏پاشی 1/0 میکرومولار) بود که به‌ترتیب موجب افزایش­­ 79، 33، 28 و 26 درصد نسبت به تیمار شاهد گردید. همچنین، بیشترین میزان رنگیزه‏های فتوسنتزی در ترکیب مذکور یافت شد، به گونه‏ای که به ترتیب موجب افزایش کلروفیل a، b، کل و کارتنوئید حدود 4/2، 1/3، 58/2 و 7/2 برابر در مقایسه­­­­­­­­­­­­ ­­با شا­هد گردید. این ترکیب سبب افزایش 35 درصدی پروتئین و 3/18 درصدی قندهای احیا نسبت به شاهد شد. نتایج حاصل از پژوهش حاضر نشان داد که 24-اپی براسینولید می‌تواند با افزایش وزن گل و اندام هوایی موجب افزایش رشد و نمو و عملکرد بستری گیاه همیشه‌بهار در فضای سبز گردد.

کلیدواژه‌ها

عنوان مقاله [English]

Investigation the response of morphological, physiological and biochemichal attributes of marigold to seed priming and 24-epibrassinolide foliar

نویسندگان [English]

  • Zahra Sardoei Kara 1
  • Vahid Reza Saffari 2
  • Iraj Tavassolian 3

1 Former M.Sc. Student, Department of Horticultural Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman - Iran

2 Associate Professor, Department of Horticultural Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman - Iran

3 Associate Professor, Department of Horticultural Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman – Iran

چکیده [English]

To investigate the effects of priming and foliar spray by 24-epibrassinolide (EBR) on some of Marigold morphological, biological and biochemical traits, an experiment was conducted as factorial on completely randomized design with three replications at experimental laboratory of department of horticulture, Shahid Bahonar University of Kerman in 2014. Each of priming and foliar spray of EBR had four concentrations (0, 0.1, 1 and 10 µM). Results showed that growth parameters and photosynthetic pigments increased proportional to the increment of EBR in priming and foliar spray treatments. . The greatest shoot weight (79 percent), root (33 percent), fresh (28 percent) and dry (26 percent) weights of pot marigold compared to control were obtained at priming with 0.1 µM plus foliar spray at 0.1 µM of EBR.   Furthermore, the highest chlorophyll a, b, total chlorophyll and carotenoids were found at the same concentration (0.1 µM priming + 0.1 µM foliar spray of EBR) with 2.4, 3.1, 2.6 and 2.7 percent increase respectively. The EBR increased protein and reduced sugar up to (35 percent) and (18.3 percent) compared with control. The results of this experiment indicated that application of EBR can be used to improve the growth and development traits of pot marigold.

کلیدواژه‌ها [English]

  • Growth regulation
  • Growth parameters
  • photosynthetic pigments
  • protein
  • reduced sugar
1 . Akram A, Aslan Khan M, Yonis A and Ashfagh M (2014) Exogenous application of 24-epibrassinolid on morphological, biochemical attributes and essential oil contents of Jasminum sambac L.. Pakistan Journal of Agricultural Sciences. 51: 881-886.
2 . Arteca JM and Arteca RN (2001) Brassinosteroid-induce exaggerated growth in hydroponically grown Arabidopsis plants. Physiolgia Plantarum. 112: 104-112.
3 . Bajgus A (2000) Effect of brassinosteroids on nucleic acids and protein content in cultured cells of Chlorella vulgaris. Plant Physiology and Biochemistry. 38: 209-215.
4 . Bajguz A and Czerpak R (1998) Physiological and biochemical role of brassinosteroids and their structure activity relationship in the green alga Chlorella vulgaris Beijerinck (Chlorophyceae). Plant Growth Regulation. 171: 131-139.
5 . Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 72: 248-254.
6 . Cag S, Goren-Baris C and Kaplan E (2007) The effect of different concentration of epibrassinolide on chlorophyll, protein and anthocyanin content and peroxidase activity in excised red cabbage (Brassica oleraceae L.) cotyledons. Biotechnology and Biotechnological Equipment. 21: 422-425.
7 . Carreon SP, Iimenez GC and Vega JL (2002) Genotoxic and antigenotoxic properties of Calendula officinalis extracts in rat liver cell cultures treated with diethylnitrosamine. Toxicology In Vitro. 16: 235-258.
8 . Catterou M, Dubis F, Schaller H, Aubanella L, Vilcol B and Sangwan-Norrel BS (2001) Brassinsteroids microtubules and cell elongation in Arabidopsis thaliana II. Effect of brassinosteroids on microtubules in the ball mutant. Planta. 212: 673-683.
9 . Chandler JW, Cole M, Flier A and Werr W (2009) BLML, a b HLH protein involved in brassinosteroid signaling controls Arabidopsis embryonic pattering via interaction with DORNRO SCHCN and DORNROSCHEN-Like. Plant Molecular Biology. 49: 153-156.
10 . Clous SD and Sasse M (1998) Brassinosteroids: essential regulators of plant growth and development. Annual Review of Plant Physiology and Plant Molecular Biology. 49: 427-451.
11 . Dole JM and Wilkins HF (2004) Floriculture: Principles and Species, 2nd ED. Pearson Prentice Hall, Upper Saddle River, New Jersey. 347p.
12 . Farduddin Q, Yusuf M and Ahmed A (2009) Effect of 28-homobrassinolid on antioxidant capacity and photosynthetic in Brassica junceae plant exposed to different levels of copper. Environomental Experimental and Botany. 66: 418-424.
13 . Hayat S, Ahmad A, Mobin M, Hussain A and Fariduddin Q (2000) Photosynthetic rate, growth, and yield of mustard plants sprayed with 28-homobrassinolide. Photosynthetica. 38: 469-471.
14 . Hayat S and Ahmad A (2011) Physiological Effects Related to Brassinostroid Application in Plant. Springer Science. 459 p.
15 . Kalinichi J, Bhushan N, Madava B and Todhunter J (1985) Relationship of nucleic acid metabolism to brassinolide-induced responses in beans. Journal of Plant Physiology. 120: 207-214.
16 . Khripach V, Zhabinskii V and Groot VAD (2000) Twenty years of brassinosteroids: steroidal plant hormones warrant better crops for the XXI century. Annals of Botany. 86: 441-447.
17 . Kommavarapu M, Parshavaneni B, Bellamkonda R and Sadhu SSR (2013) Effect of brassinosteroids on germination and seedling growth of radish (Raphanus sativus L.) under PEG-6000 induced water stress. American Journal of Plant Sciences. 4: 2305-2313.
18 . Krishna P (2003) Brassinosteroid-mediated stress responses. Journal of Plant Growth Regulation.22: 289-297.
19 . Li L and Van Staden J (1998) Effects of plant growth regulators on the antioxidant system in callus of two maize cultivars subjected to water stress. Plant Growth Regulation. 24: 55-66.
20 . Lichtenthaler HK (1987) Chlorophylls and carotenoids pigments of photosynthetic biomemberanes. Methods in Enzymology. 148: 350-382.
21 . Lim CC, Arora R and Townssenal EC (1998) Comparing comports and richards function to estimate freezing injury in Rhododendron using electrolyte leakage. Journal of the American Society for Horticultural Science. 123: 246-252.
22 . Lise A, Michelle H and Margrethe S (2004) Reduced water availability improves drought tolerane of potted miniature roses: Is the ethylene pathway involved. Department of Agricultural Sciences. Horticultural, The Royal Veterinary and Agricultureal University.
23 . Seeta Ram Rao S, Vidya Vardhini B, Sujatha E and Anuradha S (2002) Brassinosteroids – a new class of phytohormones. Current Science. 82: 10-25.
24 . Somogyi M (1952) Note on­ sugar determination.­ Journal of Biochemistry. 195: 19-29.
25 . Swamy KN and Seeta Ram Rao S (2009) Effect of 24-epibrassinolide on growth, photosynthesis, and essential oil content of Pelargonium graveolens L. Herit. Russian Journal of Plant Physiology. 52: 612-620.
26 . Swamy KN and Seeta Ram Rao S (2006) Influence of brassinosteroids on rooting and growth of geranium (Plargonium sp.) stem cutting. Asian Journal of Plant Sciences. 5: 619-622.
27 . Vandhiai BV and Seeta Ram Rao SSR (2003) Amelioration of osmotic stress by brassinosteroids on germination and seedling growth of three varieties of surgom. Plant Growth Regulation. 41: 25-30.
28 . Wu DR and Zhao YJ (1993) Effects of epibrassinolid on endogenous IAA and its oxidase in epicotyls of mung bean seedling. Acta Phytophysiologica Sinica. 19: 49-52.
29 . Yu JQ, Huang LF, Hu WH, Zhou YH, Mao WH and Ye S (2004) A role for brassinosteroids in the regulation of photosynthesis in Cucumis sativus. Journal of Experimental Botany. 55: 1135-1143.