Faezeh Samandari Bahr Aseman; Anahita Rashtian; Afagh Tabandeh Saravi; Mojtaba Soleimani Sardo
Abstract
Objective: Considering the importance (Thymus vulgaris L.) and the effects of seaweed extract and salicylic acidpriming in increasing drought resistance at different stages of plant growth, an experiment was done as a factorial layout based on a completely randomized factorial design with three replications ...
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Objective: Considering the importance (Thymus vulgaris L.) and the effects of seaweed extract and salicylic acidpriming in increasing drought resistance at different stages of plant growth, an experiment was done as a factorial layout based on a completely randomized factorial design with three replications in the research greenhouse of Jiroft University (2020).Methods: Three levels of 100% (control), 50%, and 35% field capacity were considered as test factors for drought treatment, seed preparation and seedling spraying including zero (control), 0.3%, 3.5%, and 1% algal extract and 1mM salicylic acid, 2 mM salicylic acid and composition of algae, and salicylic acid extract were considered as second factors.Results: The results of this study showed a significant difference in examined factors such as germination rate, root, and shoot length and root and shoot weight treatment at a 1% probability level and in drought stress treatment of 35%, salicylic acid priming treatment had the best performance with an improvement of 6 traits compared to the control. The results of the foliar application of seedlings also showed that the highest shoot weights of seedlings were under the combined treatment of algal extract plus salicylic acid treatment.Conclusion: General results showed that priming and foliar spraying with salicylic acid had been more effective than algae extract on plant drought resistance, and of course, the combined treatment of salicylic acid and salicylic acid extract plus algae extract with a higher concentration is able to improve plant resistance to drought in the vegetative stage.
mohsen zafaranieh; arman Azari
Abstract
The present study tries to quantify germination response of Guar (Cyamopsis tetragonoloba) to temperature and water potential. Performed in Vali-e-Asr University laboratory in 2020, it is a completely randomized design in a factorial arrangement with four replications. It aims at quantifying the rate ...
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The present study tries to quantify germination response of Guar (Cyamopsis tetragonoloba) to temperature and water potential. Performed in Vali-e-Asr University laboratory in 2020, it is a completely randomized design in a factorial arrangement with four replications. It aims at quantifying the rate of Guar germination in response to temperature, and water potential. The seeds have been germinated at seven temperatures (5, 10, 15, 20, 25, 30, and 35°C) and six water potentials (0, -0.2, -0.4, -0.6, -0.8, and -1 MPa). The results reveal that the Beta function is suitable for describing the response of seed germination temperature, and water potential. Under different water potentials, base, optimum, and maximum temperatures have been 7.3-12°C, 22.1-25.8°C, and 32.2-36°C, respectively. The hydrothermal time parameters are 268.3 bar °C h, -1.103 MPa and, 8.36°C for ɵHT (hydrothermal time constant). The data obtained from these models can be used to establish and manage guar for cultivation in different areas.
MohamMad Rahmani; Mohamad Esmaeili Aftabdari
Abstract
Considering modifications in the usual alfalfa planting pattern is critical for expansion of alfalfa seed and forage dual-purpose cultivation idea between seed growers so that, an experimental design based on randomized block design run in two years 2013 and 2014 in Zanjan province. Four planting patterns ...
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Considering modifications in the usual alfalfa planting pattern is critical for expansion of alfalfa seed and forage dual-purpose cultivation idea between seed growers so that, an experimental design based on randomized block design run in two years 2013 and 2014 in Zanjan province. Four planting patterns including hand-spreading, 25, 50 and 75 cm row spacing put on the main plots and three seeding rates including 10, 20 and 30 kg/ha put on the subplots. Results of combined analysis revealed that highest dry forage yield produced from 25 and 50 cm row spacing which had not meaningful difference with each other. The same results happened for seed rates of 20 and 30 kg/ha which had the highest dry yield. Germination as a seed quality index was not affected by planting pattern and seeding rate. But seed weight produced from 10 kg/ha seeding rate was heavier than other treatments. Economic value of dry forage was higher in 25 and 50 cm row spacing and 20 to 30 kg/ha seeding rate which were the same as each other. Based on these results, the highest economic value of alfalfa dual-purpose forage and seed production acquired from planting pattern 50 cm row spacing and 10kg/ha seeding rate and may use for alfalfa seed stand establishment as a recommendation.
Gholamhasan Movahed; Nهئش Ahmadi; Ahmad Moieni; Amin Nasiri
Abstract
In order to evaluate self and cross-incompatibility in two ecotypes of Damask rose (Rosa damascena Mill.), this experiment was performed based on randomized complete block design (RCBD) with four replications at the research field of Tarbiat Modares University in 2012-2013. In this ...
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In order to evaluate self and cross-incompatibility in two ecotypes of Damask rose (Rosa damascena Mill.), this experiment was performed based on randomized complete block design (RCBD) with four replications at the research field of Tarbiat Modares University in 2012-2013. In this experiment, twice pollinations were performed 24-hour interval, after doing in vitro testing pollen grain germination. Pollens collected from Kashan and Azaran ecotypes and Dog rose (Rosa canina) were applied as pollinizer for self- and cross-artificial pollination on both ecotypes. Determination of self and cross incompatibility were conducted with two methods of field controlled pollination and microscopic studies. Results obtained from percentage of hip development, hip weight and length, as well as seed production per hip showed that cross pollination with Dog rose pollen had the highest compatibility, comparing to pollen of Kashan and Azaran ecotypes that showed incompatibility. Also, twice pollinations had better result in comparison to one-time pollination. Field results were supported by microscopic studies based on growing pollen tube in style. According to growing pollen tube in style, just pollen tube of Dog rose could get to end of style. The concentration of acid boric (50 mg/kg) showed the highest pollen germination after 24 h, while 200 mg/kg acid boric increased pollen tube growth. According to the present results, selection of suitable pollinizer and repetition of pollination were important factors affecting Damask rose crossing, as twice controlled cross pollination by Dog rose pollen had most appropriate results on hip characteristics.
Elham Nozarpour; Reza Tavakkol afshari; Elias Soltani; Naser Majnon hoseini
Abstract
This study was conducted to evaluate the effects of water potential and temperature on seed germination rate of lemon balm (Mellissa officinalis L.). The seeds were incubated in various temperatures of 20, 23, 25, 27, 30 and 32ºC and water potentials of 0, -0.2, -0.4, -0.6 and -0.8 MPa in three ...
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This study was conducted to evaluate the effects of water potential and temperature on seed germination rate of lemon balm (Mellissa officinalis L.). The seeds were incubated in various temperatures of 20, 23, 25, 27, 30 and 32ºC and water potentials of 0, -0.2, -0.4, -0.6 and -0.8 MPa in three replications. This study was conducted in Seed Laboratory, College of Agriculture and Natural Resources, University of Tehran in Karaj in 2015. Data were analyzed using combined statistical design in a completely randomized design in several places. Segmented function was evaluated to describe cardinal temperatures. The base, optimum and ceiling temperatures of lemon balm were 17.30, 30.9 and 35ºC under optimum conditions of water potential, respectively. The base temperature increased gradually with decreasing water potential and increased to 21.25°C in -0.8 MPa water potential. The optimum temperature decreased to about 28°C by decreasing water potential to -0.6 MPa. Lemon balm seeds did not germinated in 5, 10, 15 and 35°C in any moisture levels. The R2 value of hydrothermal time model was 0.55. The hydrothermal value was 71.41 MPa oday and According to this model seed germination of lemon balm needs to 71.41 MPa oday. The results can be used for future studies on the seed biology and ecology of lemon balm.
Mohammad Kamalpouradib; Vahid Rouhi; Saadollah Houshmand; Abdolrahman Mohammadkhani; Hamid Zargari
Abstract
Pollen storage could facilitate the date palm hand pollination through saving time and space. The main concern is maintaining pollen viability, which is greatly cultivar dependent and is influenced by storage condition. In this study, to address this issue, the effects of cultivar, storage ...
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Pollen storage could facilitate the date palm hand pollination through saving time and space. The main concern is maintaining pollen viability, which is greatly cultivar dependent and is influenced by storage condition. In this study, to address this issue, the effects of cultivar, storage temperature, and time on pollen germination were investigated. A factorial experiment was laid out in a completely randomized design with three replicates using four cultivars ‘Kazeroon (7013)’, ‘Lar (7035)’, ‘Darab (7016)’ and ‘Qyr-va Karzin (7021)’, four storage temperatures (RT (20 ± 5), 4, -20 and -80°C) and six storage periods (60, 120, 150, 180, 210 and 375 days). Anova of data showed significant effects of variety, storage temperature, storage time and their interactions on pollen germination. The pollen germination rate increased with decreasing temperature. When pollens were stored for up to 150 days, the germination rates of all varieties initially increased then decreased. Overall, the optimum temperature for preserving pollen grains of tested cultivars is -80°C and the highest pollen grain longevity is related to ‘Qyr-va Karzin (7021)’ after one year.
Ozra-sadat Khatamain; Seyed Ali Mohammad Modarres-Sanavy; Batool Mahdavi; Seyed Ali Alaviasl; Younes Sharghi
Abstract
To study the effects of chitosan at 0.5 (C1), 0.1 (C2), 0.05 (C3) and 0.01 (C4) (weight percent) concentrations, distilled water (C5) and one percent acetic acid (C6) as controls on physiological and morphological characteristics of two landrace and modified (SZK-1) dragonhead (Dracocephalum moldavica ...
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To study the effects of chitosan at 0.5 (C1), 0.1 (C2), 0.05 (C3) and 0.01 (C4) (weight percent) concentrations, distilled water (C5) and one percent acetic acid (C6) as controls on physiological and morphological characteristics of two landrace and modified (SZK-1) dragonhead (Dracocephalum moldavica L.) varieties, a factorial experiment was conducted in completely randomized design in germinator of the Agronomy Department, Tarbiat Modares University. Results showed that, the interactions of treatments had significant effects on radicle length, plumule dry weigh, protein content and SOD activity. C2V2 and C6V1 produced the highest and the lowest of radicle length with 1.52 mm difference respectively. Also C2 increased the protein content of landrace variety in compare to C5. High concentrations of chitosan (C1 and C2) increased the SOD activity of modified variety compare to C5. Therefore chitosan can increase some germination characteristics of dragonhead varieties and improved bred varieties showed a better response to this organic compound.
Elias Soltani; Afshin Soltani; Mostafa Oveisi
Abstract
There are some computer programs to calculate germination related traits such as Germin which have using problems. The aim of this work was to adjust Germin for calculating germination related traits. Seed aging effects on the seedling emergence was modeled using the optimized Germin. This study was ...
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There are some computer programs to calculate germination related traits such as Germin which have using problems. The aim of this work was to adjust Germin for calculating germination related traits. Seed aging effects on the seedling emergence was modeled using the optimized Germin. This study was conducted based on completely randomized design in a factorial combination of five seeds aging treatments (0, 48, 72, 96 and 144h accelerated aging periods) and 3 levels of environmental factors. Results indicated that Chapman–Richards function adequately described cumulative emergence in all aging treatments and three environmental conditions. Emergence percentage and rate were decreased by seed aging periods from 145 to 194oC day. Results showed that observed thermal time for emergence increased as accelerated ageing periods increased and drought stress resulted in increased thermal time. For example, thermal time increased to 240oC day in 144h accelerated aged seeds in severe drought. Results indicated that with increasing thermal time in optimum condition, thermal time would increase linearly in the drought stress. Optimized program could calculate germination related traits. Therefore, it is recommended to use the optimized Germin in seed science research.
Omid Younesi; Kazem Poustini; Mohammad Reza Chaichi; Ahmad Ali Pourbabaie
Abstract
In order to study the effects of seed-bacterial priming (inoculation) on germination and early growth of alfalfa under salinity stress conditions, an experiment was conducted at seed research laboratory and Greenhouse of College of Agriculture, University of Tehran in Karaj (Iran) in 2011. The experiment ...
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In order to study the effects of seed-bacterial priming (inoculation) on germination and early growth of alfalfa under salinity stress conditions, an experiment was conducted at seed research laboratory and Greenhouse of College of Agriculture, University of Tehran in Karaj (Iran) in 2011. The experiment was arranged as a factorial in Completely Randomized Design (CRD) with three replications. Experimental treatments including: three levels of salinity stress (zero (S0), 60 (S1) and 120 (S2) mmol), two levels of alfalfa cultivars (‘Bami’ and ‘Yazdi’) and 16 levels of bacterial priming (Azetobacter, Azospirillum, Pseudomonas and Rhizobium meliloti in single and different double, triple and quadratic integrated forms). The results indicated that applying salinity stress significantly decreased germination and early seedling growth. This descending trend in control (no inoculation) treatment was more than that of treated seeds. Application of bacterial priming especially Pseudomonas priming and integrated treatments played an important role in moderating the negative effects of salinity on measured traits. According to the results of this study, it seems that plant growth promoting bacteria, by producing and releasing phytohormones such as auxin, gibberellins and cytokinin along with decreasing ethylene level, improve plant growth under salinity stress condition.
Mohammad Ali Baghestani Meibodi; Manouchehr Jam nejad; Mahdi Mainbashi Moeini; Faribi Meighani
Volume 12, Issue 2 , November 2010, , Pages 11-23
Abstract
In order to study the effect of environment temperature and GR60 concentration on seed germination and growth of Orobanche aegyptiaca and Orobanche cernua, two experiments were conducted under controlled conditions in Weed Research department of Iranian Research Institute of Plant Protection in 2008. ...
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In order to study the effect of environment temperature and GR60 concentration on seed germination and growth of Orobanche aegyptiaca and Orobanche cernua, two experiments were conducted under controlled conditions in Weed Research department of Iranian Research Institute of Plant Protection in 2008. The first experiment was established as a completely randomized design with factorial arrangement of treatments and five replications. The first factor was GR60 concentration (zero, one, two and five ppm), the second factor was broomrape species (O. aegyptiaca and O. cernua), and the third factor was temperature (10, 20 and 30?C). The second experiment was also conducted with the same statistical design and species, temperature at three levels (15, 20 and 30?C) and host plants at two levels (tomato and tobacco). Results indicated that GR60 stimulated seed germination of both broomrape species. Broomrape germination increased up to 20?C. O. cernua needed less GR60 for maximum germination compared to that of O. aegyptiaca. Stem weight of broomrape species increased by moderate increase in temperature.
Mohammad Ali Baghestani; Delavar Baghestani; Hamid Rahimian mashhadi; Hasan Mohammad Alizade
Volume 11, Issue 2 , November 2009, , Pages 19-27
Abstract
The biology and phenology of broomrape (Orobanche nana) on Almond (Prunus dulcis cv. Mamaei) was studied during the growth season in Shahrekord city of Iran in 2004. Twenty trees which were infested by broomrape, were selected. Results indicated that almonds need 2120.5 growth degree days (GDD) to complete ...
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The biology and phenology of broomrape (Orobanche nana) on Almond (Prunus dulcis cv. Mamaei) was studied during the growth season in Shahrekord city of Iran in 2004. Twenty trees which were infested by broomrape, were selected. Results indicated that almonds need 2120.5 growth degree days (GDD) to complete phenological stages from flowering to fruit ripening. Based on the cardinal temperature of broomrape (9.5°C), the parasite weed needs 209, 1125.5, 1414.5 and 961 GDD for completion of seed germination, stem emergence, flowering and seeding stages, respectively. Maximum seed germination occurred at the depth of 18 cm. Maximum root infection was observed at the depth of 9 to 12 cm. The highest infection to this parasite plant was recorded on roots with 9 to 11.9 mm diameter.
Mohammad Yazdani; Hemmatollah Pirdashti; MOhammad ALi Esmaeili; MOhammad ALi Bahmanyar
Volume 11, Issue 2 , November 2009, , Pages 83-92
Abstract
In order to investigate the residual effect of different tobacco cultivars on germination and seedling growth of rapeseed, an experiment was conducted at laboratory and glasshouse during 2007 as factorial based on completely randomized design with four replications. Extracts of root and shoot from two ...
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In order to investigate the residual effect of different tobacco cultivars on germination and seedling growth of rapeseed, an experiment was conducted at laboratory and glasshouse during 2007 as factorial based on completely randomized design with four replications. Extracts of root and shoot from two different types of tobacco (Virginia and Basma) and different concentrations of extracts (0, 40 and 80%) were the treatments. Results showed that germination percentage and rate, shoot and root length in rapeseed (Hayola 401) in laboratory experiment and root and shoot length and ratio, shoot to root dry weight ratio, leaf area, seedling emergence and vigor and chlorophyll contents were significantly affected by different tobacco root and shoot extracts. According to results root extract of Virginia and shoot extract of Basma had the highest and the lowest effect in terms of mentioned traits, respectively. Among studied characters, germination rate had highly correlation with germination percentage and seedling vigor.