In order to study the effect of environment temperature and GR60 concentration on seed germination and growth of Orobanche aegyptiaca and Orobanche cernua, two experiments were conducted under controlled conditions in Weed Research department of Iranian Research Institute of Plant Protection in 2008. The first experiment was established as a completely randomized design with factorial arrangement of treatments and five replications. The first factor was GR60 concentration (zero, one, two and five ppm), the second factor was broomrape species (O. aegyptiaca and O. cernua), and the third factor was temperature (10, 20 and 30?C). The second experiment was also conducted with the same statistical design and species, temperature at three levels (15, 20 and 30?C) and host plants at two levels (tomato and tobacco). Results indicated that GR60 stimulated seed germination of both broomrape species. Broomrape germination increased up to 20?C. O. cernua needed less GR60 for maximum germination compared to that of O. aegyptiaca. Stem weight of broomrape species increased by moderate increase in temperature.


1. مظفریان و (1388) فرهنگ نام گیاهان ایران. نشر معاصر. 740 صفحه.
2. مین‌باشی‌معینی م (1382) گل‌جالیز، گیاه‌شناسی، بیولوژی، اکولوژی و کنترل آن­ها. مؤسسه تحقیقات آفات و بیماری‌های گیاهی. 33 صفحه.
3. Awad A, Daisuke S, Dai K, Hiroaki K and Koichi Y (2006) Characterization of strigolactones, germination stimulants for the root parasitic plants Striga and Orobanche, produced by maize, millet and sorghum. J. Plant Growth Regul. 48: 221-227.
4. Barker ER, Press MC, Scholes JD and Quick WP (1996) Interactions between the parasitic angiosperm Orobanche aegyptiaca. New Phytol: 133: 637-642.
5. Borg SJ (1986) Effects of environmental factors on Orobanche-host relationships; a review and some resent results. Proceeding of the workshop on biology and control of broomrape. 12-15 August, Wageningen, Nederland. Pp. 57-69.
6. Bouwmeester H (2006) Strigolactones, signals for friends and enemies. Workshop parasitic plant management in sustainable agriculture. Final meeting of COST849, 23-24 November 2006, ITQB Oeiras-Lisbon, Portugal, Pp. 4-4.
7.  Chater AO and Webb DA (1972) Orobanche. Flora Europaea. 3: 286-293.
8. Dhanapal GNP, Ter-borg S and Struick PC (1998) Post-emergence chemical control of nodding broomrape (O. cernua) in bidi tobacco (Nicotiana tobacum) in India. Weed Technol .12 :652-659.
9. Eizenberg H, Colquhoun J and Mallory-smith CA (2004) The relationship between temperature and small broomrape (Orobache minor) parasitism in red clover (Trifolium paratense). Weed Sci. 52: 735-741.
10. Eizenberg H, Tanaami Z, Jacobsohn1 R and Rubin B (1999) Effect of carrot sowing date on parasitism of Orobanche crenata and O. aegyptiaca. Phytoparasitica. 27: 1-2.
11. El Hamouch Y, Benharrat H and Thalouran P (2006) Effect of root exudates from different tomato genotypes on broomrape (O. aegyptiaca) seed germination and tubercule development. Crop Prot. 25:501-507.
12. Gibot-Leclerc S, Corbineau F, Salle G and Come D (2004) Responsiveness of Orobanche ramosa L. seeds to GR 24 as related to temperature, oxygen availability and water potential during preconditioning and subsequent germination. J. Plant Growth Regul. 43:63–71.
13. Gomez-Roldan V, Fermas S, Brewer PB, Puech-Pagès V, Dun EA, Pillot J, Letisse F, Matusova R, Danoun S, Portais J, Bouwmeester H, Bécard G, Beveridge CA, Rameau C and Rochange SF (2008) Strigolactone inhibition of shoot branching. Nature. 455: 189-194.
14. Hipkinson JM (1967) Effects of night temperature on the growth of Nicotiana tabacum. Aust. J. Exp. Agr. Anim. Husbandry. 7: 78–82.
15. Hsuck C, Mueller S and Schildknecht H (1992) A germination stimulant for parasitic flowering plants from Sorghum bicolo, a genuine host plant. J. Plant Physiol. 139: 474-478.
16. Johanson AW, Rosebery G and Parker C (1976) A novel approach to Striga and Orobanche control using synthetic germination stimulants. Weed Res. 16: 223-227.
17. Kadra E (1999) Modeling of the effect of water stress and temperature on germination rate of Orobanche aegyptiaca seed. Ph.D. Thesis, Reading University. 217 p.
18. Kassasian L (1973) Control of Orobanche. PANS. 19: 368-371.
19. Khosh-khui M, Shaybani B, Rouhani I and Tafazoli E (1985) Principles of horticulture. Shiraz University Press. 553 p.
20. Matusova R and Bouwmeester H (2006) Germination stimulant(s) perception by parasitic plants. Workshop parasitic plant management in sustainable agriculture. Final meeting of COST849, 23-24 November 2006, ITQB Oeiras-Lisbon, Portugal, Pp. 5-6.
21 . Mauromicale G, Monaco AL and Longo AMG (2008) Effect of branched broomrape (Orobanche ramosa) infection on the growth and photosynthesis of tomato. Weed Sci. 56: 574–581
22. Musselman LJ (1980) The biology of Striga, Orobanche, and other root-parasistic weeds. Annu. Rev. Phytopathol. 18: 463-489.
23. Parker C and Riches CR (1993) Parasitic weeds of the world: Biology and control. CAB Int., Wallingford, UK.
24. Plakhine D, Goldwasser Y, Eizenberg H, Kleifeld Y and Hershenhorn J (2002) The influence of temperature on Orobanche resistance. Proceedings of the meeting “Broomrape: biology and resistance. 14-18 March, Sofia, Bulgaria. Pp. 11-12.
25. Razavi Z (1984) Chemical synthesis of germination factors. Iran. J. Chem. and Chem. Eng. 19: 38-44.
26. Saghir A and Abu–Shakra R (1971) Effect of diphenamid and trifluralin on the germination of Orobanche seeds in vitro.Weed Res. 11: 74-76.
27. Sauerborn J (1991) The economic importance of the phytoparasite Orobanche and Striga. In: Ransom JK, Musselman LJ, Worsham AO and Parker C W (Eds.), Proceeding 5th International Symposium in Parasitic weeds. CIMMYT, Nariobi, Kenya. Pp. 137-143.
28. Sauerborn J, Saxena MC and Mayer A (1989) Broomrape control in faba bean (V. faba) with glyphosate and   imazequine.Weed Res. 29: 97-102.
29. Sauerborn J, Saxena MC and Masri K (1987) Control of Orobanche spp. with scepter herbicides. FABI News. 19: 14-17.
30. Song WJ, Zhou WJ, Jin ZL, Cao DD, Joel DM, Takeuchi Y and Yoneyamak K (2005) Germination of Orobanche seeds subjected to conditioning temperature, water potential and growth regulator treatments. Weed Res. 45: 467-476.
31. Sukno S and Fernández-Martínez JM (2001) Temperature effects on the disease reactions of sunflower to infection by Orobanche cumana. Plant disease. 85: 553-556.
32. Van Hezewijk MJ, Vanbeen AP and Verkeij JAC (1993) Germination of O.crenata, as influenced by conditioning temperature and period. Can. J. Bot. 71: 786-792.
33. Vurro MA, Boari A, Pilgram AL and Sands DC (2006) Exogenous amino-acids inhibit seed germination and tubercule formation by Orobanche ramosa: Potential application for management of parasitic weeds. Biol. control. 36: 258-265.
34. Wegmann K (2006) Germination physiology as a target for Orobanche control. Workshop parasitic plant management in sustainable agriculture. Final meeting of COST849, 23-24 November 2006, ITQB Oeiras-Lisbon, Portugal. Pp. 2-3.
35. Wigchert SC, Kuiper E, Boelhouwer GJ, Nefkens GH, Verkleij JAC and Zwane B (1999) Dose response of seeds of the parasitic weeds Striga and Orobanche to germination stimulants GR24 and Nijmegen. J. Agric. Food Chem. 47: 1705-1710.
36. Zehhar N, Ingouff M, Bouya D and Fer A (2002) Involvement of gibberellins and ethylene in Orobanche ramosa germination. Weed Res. 42: 464-469.