Raheleh Ghale Ghafi; Hossein HajiAbaee; Fathieh Nabhani; Salvia Mohammadpour; Zahra Ardanji Kalate Siyahdasht
Abstract
Objective: This study was conducted with the aim of investigating the influence of mycorrhizal fungi (Rhizophagus irigularis) and rhizobacteria (Pseudomonas fluorescens) inoculation on maize under different fertilization conditions.Methods: This study was carried out in two separate experiments under ...
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Objective: This study was conducted with the aim of investigating the influence of mycorrhizal fungi (Rhizophagus irigularis) and rhizobacteria (Pseudomonas fluorescens) inoculation on maize under different fertilization conditions.Methods: This study was carried out in two separate experiments under greenhouse settings in 2018. The first experiment aimed to compare the conventional soil inoculation method with microbial seed coating, and the second experiment strived to assess the growth of maize inoculated with mycorrhizal fungi and plant growth-promoting rhizobacteria via microbial seed coating at three treatment levels of Hoagland nutrient solution (Control, 80 and 100 volumetric percentage of Hoagland solution). In both experiments, one gram of maize roots and adhering soil were sampled 70 days after microbial seed coating and the available DNA was extracted from the maize rhizosphere.Results: In the first experiment, no significant difference was observed between conventional soil inoculation and microbial seed coating concerning the dry weight of stems and roots, longitudinal colonization percentage of the roots, arbuscule abundance, vesicles abundance, and elemental concentration. In the second experiment and under full fertilization condition, mycorrhizal inoculation resulted in a significant increase in concentrations of nitrogen, phosphorus, potassium, and zinc (18.1, 3.5, 56 and 46.0 mg/kg, respectively) compared to the control treatment (12.6, 3.1, 39.6, and 24.4 mg/kg, respectively), and the bacterial inoculation exhibited a significant increase for magnesium, zinc, and manganese (2.0, 42.6, and 145 mg/kg, respectively) compared to the control treatment (1.0, 24.4, and 60 mg/kg, respectively).Conclusion: Microbial seed coating could be regarded as a novel approach of large-scale inoculation of microorganisms in maize production.
tayebe rostami; hamid abbasdokht; hassan makarian; Manoochehr Gholipoor; khalil karimzadehasl
Abstract
Objective: This study aimed at assessing the effects of mycorrhiza and phosphorus fertilizer on morphophysiological traits and yield of quinoa (Chenopodium quinoa Willd.) and maize (Zea mays L.) in an intercropping system by using of replacement method.Methods: A complete randomized block design (CRBD), ...
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Objective: This study aimed at assessing the effects of mycorrhiza and phosphorus fertilizer on morphophysiological traits and yield of quinoa (Chenopodium quinoa Willd.) and maize (Zea mays L.) in an intercropping system by using of replacement method.Methods: A complete randomized block design (CRBD), with three replications was implemented at two research farms affiliated with Shahrood University of Technology, located in Shahrood and Miami cities. The intercropping systems consisted of five cropping ratios: pure quinoa and maize cultivation, 75% quinoa + 25% maize, 50% quinoa + 50% maize, 25% quinoa + 75% maize. The first factor and phosphorus at three levels (zero, 50 and 100 kgha-1) and mycorrhiza at two levels (use and unuse) were considered as the second and third factors.Results: The results demonstrated that most of studied traits were significantly influenced by the intercropping systems, mycorrhiza application, and phosphorus concentrations. The highest plant height in maize (202.8 cm) and quinoa (81.3 cm) was obtained by use of mycorrhiza and phosphorus fertilizer 50 kgha-1. Similarly, the highest leaf area index in quinoa (86.2) and maize (6.57) amount of chlorophyll in quinoa and maize respectively (2.88 and 12.39 mg/ml) were obtained in Shahrood region by using mycorrhiza and 50 kgha-1 phosphorus fertilizer in cropping ratio 50 %.Conclusion: According to the results, the effects of different intercropping ratio, use of mycorrhiza, and phosphorus fertilizers had a significant impact on the morphophysiological characteristics of quinoa and maize.
mehran beygi kharvani; Ali Akbar Safari Sinegani
Abstract
A study was conducted as split plot layout with three replications at the research greenhouse of Bu-Ali Sina University, Hamedan in 2017 in order to investigate the consequences of using biochar and hydrochar, produced from potato plant residue on bean plant growth indices, chlorophyll content, and root ...
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A study was conducted as split plot layout with three replications at the research greenhouse of Bu-Ali Sina University, Hamedan in 2017 in order to investigate the consequences of using biochar and hydrochar, produced from potato plant residue on bean plant growth indices, chlorophyll content, and root mycorrhizal symbiosis in drought stress. The main plot and subplots in this study have been two drought levels and four amendment treatments, respectively. The study shows that the drought stress and the application amendments in various forms have had significant effects on plant growth indices, chlorophyll content, and root mycorrhizal colonization. Drought stress reduces root and shoot dry weights up to 39.8, 46.1%, leaf chlorophyll a (Chl a) content up to 52.6%, chlorophyll b (Chl b) content up to 58%, and total chlorophyll up to 54.52%. Although the number of rhizobium nodule on the root of plant decreases in drought stress, the rate of root mycorrhiza rises by 19.2% in drought stress. The use of biochar increased the mycorrhizal symbiotic indices significantly. It increases root colonization 11.34% and Glomeromycota spore number 50.5% in soil. The application of raw residue in soil has had the most positive effects on the plant growth indices and the leaf chlorophyll contents, leading to increased shoot dry weights (49.8%) and chlorophyll a, b and total contents (3.54%, 36.8%, and 14.5% respectively). The findings of this study show that among the treatments, the best plant growth index has been obtained in the use of potato biochar, which reduces the harmful effects of drought stress on the bean plant.
mrs Somayeh Mirzaei; Seyed Ataollah Siadat; Babak pakdaman sardrod,; Mohammad Moradi Telavat
Abstract
In order to investigate the effect of Piriformospora indica and different levels of salicylic acid on morphological characteristics and activity of antioxidant enzymes of thyme (Thymus vulgaris L.) irrigation cut-off stress, an experiment has been conducted in the water year 2016. It has employed a split ...
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In order to investigate the effect of Piriformospora indica and different levels of salicylic acid on morphological characteristics and activity of antioxidant enzymes of thyme (Thymus vulgaris L.) irrigation cut-off stress, an experiment has been conducted in the water year 2016. It has employed a split factorial experiment based on randomized complete block design with four replications in the research farm of Dehloran Agricultural Research Center. The treatments are consisted of four levels of salicylic acid (0, 150, 300, and 600 μm) as foliar application and a mycorrhiza fungus agent at two levels of inoculation and non-inoculation and irrigation cut-off stress at three levels (irrigation at 90%, 50%, and 30% filed capacity). The results of this study show that with increasing irrigation cut-off stress up to 30% filed capacity plant height, total weight of shoot, number of leaves, colonization percentage, and the enzymes Catalase and Peroxidase have decreased. Inoculation and treatment with salicylic acid up to a concentration of 300 μM could significantly reduce the destructive effects of irrigation cut-off stress on these traits. However, increasing the concentration of salicylic acid to the level of 600 μM intensifies the effects of irrigation cut-off stress, preventing the positive effects of the fungus.
Raheleh Sadat Aletaha Maki; Ali Akbar Safari Sinegani; Doustmorad Zafari
Abstract
In highly stressed ecosystems, symbiosis between plants and has beneficial effects on plant growth. The objective of this study was the effects of two dark septate endophyt fungi against mycorrhizal (Glomus mussea and Glomus interaradices) and Piriformospora indica on Spinach growth in without and with ...
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In highly stressed ecosystems, symbiosis between plants and has beneficial effects on plant growth. The objective of this study was the effects of two dark septate endophyt fungi against mycorrhizal (Glomus mussea and Glomus interaradices) and Piriformospora indica on Spinach growth in without and with drough stress (Field Capacity (FC) and 50% FC). This study was done at Bu- Ali sina university of Hamedan greenhouse (2015-2016). The percentage of root colonization, wet and dry weights, contents of chlorophyll and some of micro- and macronutrients have been analysed in some adult treated plants after six weeks. The colonization results showed that all studied fungi can effectively enter into spinach roots. Inoculation with Glomus mussea increased the wet weight (20 g/plant) of the plants. Also, some morphophysiological and physological properties such as dry weight (4.1 g/plant), chlorophyll content (2.7 mg/gwd) and potassium to sodium ratio (8.4) were increased when plants inoculated with Curvularia specifera. However, high phosphorus content (311.2 mg/100gwd) measured in plants that was inoculated with Glomus interaradices. Generally, this study shows dark septate endophyte could be benefical in grwoth of spinach as much as mycorrhizal and Piriformospora indica symbiosis.