Document Type : Research Paper

Authors

1 Ph.D. Student, Department of Soil Science, Faculty of Agriculture, Bu-Ali Sina University, Hamadan, Iran

2 Professor, Department of Soil Science, Faculty of Agriculture, Bu-Ali Sina University, Hamadan, Iran

3 Associate Professor, Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamadan, Iran.

Abstract

In highly stressed ecosystems, symbiosis between plants and has beneficial effects on plant growth. The objective of this study was the effects of two dark septate endophyt fungi against mycorrhizal (Glomus mussea and Glomus interaradices) and Piriformospora indica on Spinach growth in without and with drough stress (Field Capacity (FC) and 50% FC). This study was done at Bu- Ali sina university of Hamedan greenhouse (2015-2016). The percentage of root colonization, wet and dry weights, contents of chlorophyll and some of micro- and macronutrients have been analysed in some adult treated plants after six weeks. The colonization results showed that all studied fungi can effectively enter into spinach roots. Inoculation with Glomus mussea increased the wet weight (20 g/plant) of the plants. Also, some morphophysiological and physological properties such as dry weight (4.1 g/plant), chlorophyll content (2.7 mg/gwd) and potassium to sodium ratio (8.4) were increased when plants inoculated with Curvularia specifera. However, high phosphorus content (311.2 mg/100gwd) measured in plants that was inoculated with Glomus interaradices. Generally, this study shows dark septate endophyte could be benefical in grwoth of spinach as much as mycorrhizal and Piriformospora indica symbiosis.

Keywords

اسماعیل‌زاده ص (1384) بررسی جمعیت‌های میکوریزی پارک ملی تندوره خراسان و تأثیر عناصر کلسیم و پتاسیم بر میکوریزایی شدن درشرایط آزمایشگاهی. دانشگاه تریت مدرس. پایان­نامه کارشناسی ارشد.
الیاسی یگانه م (1392) بررسی همزیستی قارچ‌های میکوریزی آربوسکولار در برخی از گیاهان دارویی و سبزی‌های همدان. دانشگاه بوعلی سینا. پایان‌نامه کارشناسی ارشد.
جلالی ا و جعفری پ (1395) تأثیر محلول‌پاشی پتاسیم در کاهش اثرات مضر شوری در گیاه اسفناج. نشریه علوم باغبانی.  30(2): 201-208.
جهاندیده مهجن آبادی و و سپهری م (1393) اثر تلقیح قارچ Piriformos poraindica بر جذب و انتقال برخی عناصر در دو رقم گندم. مدیرت خاک و تولید پایدار. 4(3): 155-173.
حاجی نیا س، زارع م ج، محمدی گل تپه ا و رجالی ف (1390) بررسی سودمندی قارچ اندوفیت Piriformos poraindica و باکتری  Azospir illum Sp. در افزایش تحمل گندم رقم سرداری به تنش شوری. تنش‌های محیطی در علوم زراعی. 4(1): 21-31.
حبیبی س، فرزانه م و مسکرباشی م (1392) تأثیر قارچ مایکوریز بر رشد و جذب عناصر غذایی گندم در شرایط شور. تحقیقات آب و خاک ایران. 44(3): 311-320.
خراسانی نژاد س، سلطانلو ح، رمضانپور س، هادیان ج و آتشی ص (1394) اثر تنش خشکی روی برخی خصوصیات مورفولوژیکی کمیت و کیفیت اسانس در اسطوخودوس. به‌زراعی کشاورزی. 17(4): 979-988.
رضوانی م، افشنگ ب، قلی زاده ع و زعفریان ف (1390) ارزیابی تأثیر قارچ مایکوریزا و منابع مختلف فسفر بر رشد و جذب فسفر در سویا. مدیریت خاک و تولید پایدار. 1(2): 97- 117.
زنگنه س (1391) بررسی وضعیت همزیستی مایکوریزایی چند گیاه غالب در بیابان­های استان سمنان. رستنی‌ها. 3(1): 105- 108.
سادات ع، ثواقبی غ، رجالی ف، فرحبخش م، خاوازی ک و شیرمردی م (1389) تأثیر چند نوع قارچ مایکوریز آربوسکولار و باکتری محرک رشد بر شاخص­های رشد و عملکرد دو رقم گندم در یک خاک شور. نشریه آب و خاک. 24(1): 53-62.
سلیمانی ف، احمدوند گ و صفری سنجانی ع ا (1396) تأثیر کودهای شیمیایی، زیستی و آلی بر شاخص­های رشدی، عملکرد و اجزای عملکرد آفتابگردان تحت شرایط آبیاری کم و بهینه. دانش کشاورزی و تولید پایدار. 27(2): 19- 35.
نامور پ (1393) تأثیر قارچ Piriformos poraindica بر رشد و جذب نیتروژن در گیاهان ذرت. دانشگاه فردوسی مشهد. پایان‌نامه کارشناسی ارشد.
Akhani H (2006) Biodiversity of halophytic and sabkha ecosystems in Iran. In: Sabkha ecosystems Volume II: West and Central Asia, Ajmal Khan M (Ed.). Springer. pp. 71-88.
Arzanesh M H, Alikhani H A, Khavazi K, Rahimian HA and Miransari M (2011) Wheat (Triticum aestivum L.) growth enhancement by Azospirillum sp. under drought stress. World Journal of Microbiology and  Biotechnology. 27: 197-205.
Assadi M (2001) Chenopodiaceae. Flora of Iran. 38: 260-261.
Augé Robert M (2001) Water relations, drought and vesicular-arbuscular mycorrhizal symbiosis. Mycorrhiza. 11: 3-42.
Barrow JR, Havstad KM, Hubstenberger J and McCaslin BD (1997b) Seed borne fungal endophytes on fourwing saltbush, Atriplex canescens. Arid Land Research and Management. 11: 307-314.
Barrow JR (2001) Carbon Transport by Symbiotic Fungi in Fourwing Saltbush, Atriplex canescens (Pursh) Nutt. In Wildland Shrub and Arid Land Restoration Symposium Proceedings.  Pp: 1-4.
Barrow JR and Osuna P (2002) Phosphorus solubilization and uptake by dark septate fungi in fourwing saltbush, Atriplex canescens (Pursh) Nutt. Journal of Arid Environments. 51: 449-459.
Barrow JR, Lucero M, Osuna Avila P, Reyes Vera I and Aaltonen RE (2007) In: Fungal genomes that influence Basic Physiological Processesa of black grama and fourwing salt bush in arid south-western rangelands; Proceedings: Shrubland dynamics-fire and water; 2004 August 10–12; Lubbock, TX Proceedings RMRS-P-47. Fort Collins, CO; US Department of Agriculture, Forest Service, Rocky Mountain Research Station. p. 173.
Barrow J, and Aaltonen R (2001) Evaluation of the internal colonization of Atriplex canescens (Pursh) Nutt. roots by dark septate fungi and the influence of host physiological activity. Mycorrhiza. 11: 199-205.
Beis A and Patakas A (2012) Relative contribution of photoprotection and anti-oxidative mechanisms to differential drought adaptation ability in grapevines. Environmental and Experimental Botany. 78: 173-183.
Carroll GC and Carroll FE (1978) Studies on the incidence of coniferous needle endophytes in the Pacific Northwest. Canadian Journal of Botany. 56: 3034-3043.
Dougherty RC, Strain HH, Svec WA, Uphaus RA andKatz JJ (1966) Structure of chlorophyll c1. Journal of the American Chemical Society. 88: 5037-5038.
Ellis M (1976) More Dematiaceus hyphomycetes. Commowealth mycological Institute, Key (England)
Garcia E, Alonso A, Platas G and Sacristan S (2013) The endophytic mycobiota of Arabidopsis thaliana . Fungal Diversity. 60: 71- 89.
Ghanem G, Ewald A, Zerche S and Hennig F (2014) Effect of root colonization with Piriformospora indica and phosphate availability on the growth and reproductive biology of a Cyclamen persicum cultivar. Scientia Horticulturae. 172: 233-241.
Giri B, Kapoor R and Mukerji KG (2007) Improved tolerance of Acacia nilotica to salt stress by arbuscular mycorrhiza, Glomus fasciculatum may be partly related to elevated K/Na ratios in root andshoot tissues. Microbial Ecology. 54: 753-760.
Hammer EC, Nasr H, Pallon J, Olsson PA and Wallander H (2011) Elemental composition of Haselwandter K. and  Read DJ. 1982. The significance of rootfungus association in two Carex species of high-alpine plant communities. Oecologia. 53: 352-354.
Haselwandter K and Read DJ (1982) The significance of rootfungus association in two Carex species of high-alpine plant communities. Oecologia. 53: 352-354.
Jumpponen A (2001) Dark septate endophytes-are they mycorrhizal?. Mycorrhiza. 11: 207-211.
Mandyam K and Jumpponen A (2005) Seeking the elusive function of the root-colonising dark septate endophytic fungi. Studies in Mycology. 53: 173-189.
Mardukhi B, Rejali F, Daei G, Ardakani MR, Malakouti MJ and Miransari M (2011) Arbuscular mycorrhizas enhance nutrient uptake in different wheat genotypes at high salinity levels under field and greenhouse conditions. Comptes Rendus Biologies. 334: 564-571.
Mathur N and Vyas A (2000) Influence of arbuscular mycorrhizae on biomass production, nutrient uptake and physiological changes in Ziziphus mauritiana Lam. under water stress. Journal of Arid Environments. 45: 191-195.
Mondale RK and Paul NK (2000) Effect of oil moisture on growth attributes root characters and yield of mustard (Brassica juncea L.). Pakistan Journal of Botany. 27: 143-150.
Newsham KK, Upson R and Read DJ (2009) Mycorrhizas and dark septate root endophytes in polar regions. Fungal Ecology. 2: 10-20.
Posada F, Catherine Aime M, Peterson SW, Rehner SA and Vega FE (2007) Inoculation of coffee plants with the fungal entomopathogen Beauveria bassiana (Ascomycota: Hypocreales). Mycological research. 3: 748-757.
Safari Sinegani AA and Elyasi Yeganeh M (2017) The occurrence of arbuscular mycorrhizal fungi in soil and root of medicinal plants in Bu-Ali Sina garden in Hamadan, Iran. Biological Journal of Microorganism. 5: 43-59.
Sengupta A and Chaudhuri S (2002) Arbuscular mycorrhizal relationships of mangrove plant community at theGanges river estuary in India. Mycorrhiza. 12: 169-174.
Sieber TN (2007) Endophytic fungi in forest trees: are they mutualists? Fungal Biology Reviews. 21: 75-89.
Sieber TN and Grünig CR (2006) Biodiversity of fungal root-endophyte communities and populations, in particular of the dark septate endophyte Phialocephala fortinii sl, Microbial root endophytes. Springer. pp. 107-13.
Smith SE and Read  DJ (2008) Mycorrhizal Symbiosis. 3rd Ed. Academic Press, London, UK.
Swift RS, Sparks DL (1996) Methods of soil analysis: Part 3. Chemical methods. Soil Science Society of America Book Series. 5: 1018-1020.
Woudenberg JHC, Groenewald JZ, Binder M and Crous PW (2013) Alternaria redefined. Studies in Mycology. 75: 171-212.