Document Type : Research Paper
Authors
- Raheleh Ghale Ghafi 1
- Hossein HajiAbaee 2
- Fathieh Nabhani 3
- Salvia Mohammadpour 4
- Zahra Ardanji Kalate Siyahdasht 5
1 Graduated from the Islamic Azad University gorgan with a masters degree in agriculture
2 Former M.Sc. Student of Weed Science, Agronomy and Plant Breeding Department, Agriculture and Natural Resources Campus, University of Tehran, Karaj, Iran
3 Graduated from Shushtar Islamic Azad University
4 Faculty of Agronomy Department, Agriculture Campus, Baharan Higher Education Institute, Gorgan, Iran
5 Former M.Sc. Student of Agricultural Biotechnology, University Agriculture Shirvan, North Khorasan, Iran
Abstract
This study was carried out to investigate the effect of mycorrhizal fungi (Rhizophagus irigularis) and rhizobacteria (Pseudomonas fluorescens) on maize under different fertilization conditions as greenhouse research in the Jalin region of Gorgan in 2018 during two separate experiments. The aim of the first experiment was to compare the conventional soil incubation with seed-coating incubation, and the second experiment was to assess the growth of maize incubated with mycorrhizal fungi and plant growth-promoting rhizobacteria through seed-coating at three treatment levels of Hoagland nutrient solution (Control, 80 and 100 volumetric percentage of Hoagland solution). In both experiments, at 70 days after seed-coating, one gram of maize roots and soil attached to it were sampled and DNA was extracted from the maize rhizosphere. In the first experiment, there was no significant difference between the conventional soil incubation and seed-coating incubation according to the dry weight of stems and roots, roots’ longitudinal colonization percentage, arboscol abundance, vesicles abundance, and elements concentration. In the second experiment and under full fertilizing conditions, mycorrhizal incubation showed a significant increase in concentrations of nitrogen, phosphorus, potassium, and zinc (18.1, 3.5, 56, and 46.0 mg/kg, respectively) compared to the control (12.6, 3.1, 39.6, and 24.4 mg/kg, respectively), and the bacterial incubation showed a significant increase for magnesium, zinc, and manganese (2.0, 42.6, and 145 mg/kg, respectively) compared to the control (1.0, 24.4, and 60 mg/kg, respectively).
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