Document Type : Research Paper


1 M.Sc. Student, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, Iran

2 Assistant Professor, National Salinity Research Center, Agricultural Research, Education and Extension Organization (AREEO), Yazd, Iran

3 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture & Natural Resources, Ardakan University, Ardakan, Iran


To evaluate the effect of salinity stress on some of growth characteristics of selected pomegranate (Punica granatum) genotypes of regains with salinity water and soil, a factorial experiment was carried out based on completely randomized design (CRD), with two factors; genotypes in 4 levels (‘Vahshi Babolsar’, ‘Narak Lasjerd Semnan’, ‘Chah Afzal’ and ‘Voshik Torsh Saravan’ genotypes and irrigation water salinity in 5 levels (1, 3, 5, 7 and 9 dS/m respectively). The results showed that type of genotype and level of salinity affected morphological and physiological characteristics and concentration of leaves element nutrient. In all of the studied genotypes, with increasing of salinity concentration, growth characteristics including branch height, branch diameter, number of total leaves, green leaves percentage, aerial organs fresh and dry weight, relative humidity percentage, SPAD, contents of a, b and total chlorophylls reduced. But, necrotic leaves percentage, downfall leaves percentage, root fresh weight to aerial organs fresh weight ratio, relative ionic percentage, Na+ and cl- percentage and Na+ to K+ ratio increased. Overall, ‘Chah Afzal’ and ‘Voshik Torsh Saravan’ genotypes were recognized as the most tolerant and sensitive genotypes to salinity stress, in respectively. ‘Chah Afzal’ genotype could be tolerated salinity and 7 dS/m by maintaining its growth characteristics and increasing potassium uptake against sodium.


Arnon, D.I. (1949). Copper enzymes in isolated chloroplast polyphenoloxidase in Beta vulgaris. Plant Physiology, 24, 1-15.
Emami, A. (1996). Methods of plant analysis. Agricultural Research and Education Organization. Soil and Water Institute. 130 Pp. (In Persian)
Fipps, G. (2003). Irrigation water quality standards and salinity management strategies. Texas Agricultural Extension Service, Pp 1-18.
Guo, F. O. & Tang, Z. C. (1999). Reduced Na+ and K+ permeability of K+ channel in plasma membrane isolated from roots of salt tolerant mutant of wheat. Chinese Academy of Sciences, 44(9), 816-821.
Heiydari Sharif Abad, H. (2001). Plant and salinity. Research Institute of Forests and Rangelands. 71 Pp. (In Persian).
Ibrahim, H. I. M. (2016). Tolerance of two pomegranates cultivars (Punica granatum L.) to salinity stress under hydroponic culture conditions. Journal of Basic and Applied Scientific Research, 6(4), 38-46.
Karakas. B., Bianco, R.L. & Rieger, M. (2000). Association of marginal leaf scorches with sodium accumulation in salt-stressed peach. Journal of the American Society for Horticultural Science, 35(1), 83-84. DOI: https://
Liu, C., Ming, Y., Xianbin, H. & Zhaohe, Y. (2018). Effects of salt stress on growth and physiological characteristics of pomegranate (Punica granatum L.) cuttings. Pakistan Journal of Botany, 50(2), 457-464.
Lutts, S., Kinet, J.M. & Bouharmont, J. (1995). Changes in plant response to NaCl during development of rice (Oryza sativa L.) varieties differing in salinity resistance. Journal of Experimental Botany, 46, 1843-1852. https://
Karimi, H.R. & Hasanpour, Z. 2014. Effects of salinity and water stress on growth and macro nutrients concentration of pomegranate (Punica granatum L.). Journal of Plant Nutrition, 37, 1937-1951.
Maas, E. V. & Hoffman, G. J. (1977) Crop salt tolerance: current assessment. Journal of Irrigation and Drainage Engineering, 103(2), 115- 134.    
Mahajan, Sh. & Tuteja, N.) 2005). Cold, salinity and drought stresses: An overview. Archives of Biochemistry and Biophysics, 444(2), 139-158. DOI: 10.1016/
Massai, R., Remorni, D. & Tattini, M. (2004). Gas exchange, water relations and osmotic adjustment in two scion/rootstock combinations of Prunus under various salinity concentrations. Journal of Plant and Soil Science, 259(1-2), 153-162.
Mastrogiannidou, E., Chatzissavvidis, C., Antonopoulou, C., Tsabardoukas, V., Giannakoula, A. & Therios, I. (2016). Response of pomegranate cv. wonderful plants tο salinity. Journal of Soil Science and Plant Nutrition, 16(3),621-636.  
Momenpour, A. & Imani, A. (2018). Evaluation of salinity tolerance in fourteen selected pistachio (Pistacia vera L.) cultivars. Advances in Horticultural Science, 32(2), 249-264. DOI:
Momenpour, A., Bakhshi, D., Imani, A. & Rezaie, H. (2015 a). Effect of salinity stress on growth characteristics and concentrations of nutrition elements in Almond (Prunus dulcis) ‘Shahrood 12’, ‘Touno’ cultivars and ‘1-16’ genotype budded on GF677 rootstock. Journal of Agricultural Crops Production, 17(1), 112-133. (In Persian). DOI: 10.22059/jci.2015.54798.
Momenpour, A., Bakhshi, D., Imani, A. & Rezaie, H. (2015b). Effect of salinity stress on the morphological and physiological characteristic in some selected almond (Prunus dulcis) genotypes budded on GF677 rootstock. Plant Production Technology, 7(2), 137-152. (In Persian)
Momenpour, A., Imani, A., Bakhshi, D. & Akbarpour, E. (2018). Evaluation of salinity tolerance of some selected almond genotypes budded on GF677 rootstock. International Journal of Fruit Science, 18(4), 410-435.  DOI /abs/10.1080/15538362.2018.1468850.
Momenpour, A., Imani, A., Bakhshi, D. & Rezaie, H. (2015 c). Effect of salinity stress on concentrations of nutrition elements in almond (Prunus dulcis) 'Shokofeh', 'Sahand' cultivars and '13-40' genotype budded on GF677 rootstock. Iranian Journal of horticulture science, 29(2), 255-268. (In Persian). DOI: 10.22059/ijhs.2015.55862.
Momenpour, A., Imani, A., Bakhshi, D. & Rezaie, H. (2015 d). Evaluation of salinity tolerance in some almond genotypes grafted on GF677 rootstock base on morphological characteristic and chlorophyll fluorescence. Journal of Plant Process and function, 3 (10), 9-28. (In Persian)
Munns, R. (2002). Comparative physiology of salt and water stress. Plant, Cell and Environment, 25(2), 239-250.
Munns, R. & Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59, 651-681. DOI: 10.1146/annurev.arplant.59.032607.092911.
Ruiz-Sanchez, M., Domingo, R. & Castel, G. (2010). Review deficit irrigation in fruit trees and vines in Spain. Spanish Journal of Agricultural Research, 8(2), 5-20.
Naeini, M. R., Khoshgoftarmanesh, A.H., Lessani, H. & Fallahi, E. (2005). Effects of sodium chloride-induced salinity on mineral nutrients and soluble sugars in three commercial cultivars of pomegranate. Journal of Plant Nutrition, 27(8), 1319-1326.
Okhovatian-Ardakani, A.R., Mehrabanian, M., Dehghani, F. & Akbarzadeh, A. (2010). Salt tolerance evaluation and relative comparison in cuttings of different pomegranate cultivars. Plant, Soil and Environment, 56(4), 176-185.
Rahemi, M., Nagafian, Sh. & Tavallaie, V. (2008). Growth and chemical composition of hybrid GF677 influenced by salinity levels of irrigation water. Journal of Plant Sciences, 7(3), 309-313. DOI: 10.3923/ajps.2008.309.313.
Rahmani, A., Daneshvar, H. A. & Sardabi, H. (2003). Effect of salinity on growth of two wild almond species and two genotypes of the cultivated almond species (P. dulcis). Iranian Journal of Forest and Poplar Research, 11(1), 202-208. (In Persian).  DOI: 10.22092/ijfpr.2003.109298.
Shibili, R.A., Shatnawi, M.A. & Swaidat, I.Q. (2003). Growth, osmotic adjustment and nutrient acquisition of bitter almond under induced sodium chloride salinity in vitro. Communications in Soil Science and Plant Analysis, 34, 1969-1979. DOI /abs/10.1081/CSS-120023231
Szczerba, M.W., Britto, D. T. & Kronzucker, H. J. (2009). K+ transport in plants: physiology and molecular biology. Journal of Plant Physiology, 166(5), 447-466. DOI: 10.1016/j.jplph.2008.12.009.
Szczerba, M. W., Britto, D. T., Balkos, K. D. & Kronzucker, H. J. (2008). NH4+ stimulated and -inhibited components of K+ transport in rice (Oryza sativa L.). Journal of Experimental Botany, 59(12): 3415–3423. DOI: 10.1093/jxb/ern190.
Tavousi, M., Kaveh, F., Alizadeh, A., Babazadeh, H. & Tehranifar, A. (2016). Effect of salinity and deficit irrigation on quantity and quality of pomegranate (Punica granatum L.). Iranian Journal of Irrigation and Drainage, 4(10), 499-507. (In Persian).
Yamasaki, S. & Dillenburg, L.C. (1999). Measurements of leaf relative water content in Araucaria angustifolia. Revista Brasilian Fisiologia Vegetal, 11(2), 69-75.