This study was performed to investigate the effect of four types of binders at different levels: carboxymethylcellulose (40, 60 and 80 g/l), arabic gum (50, 100 and 150 g/l), tragacanth (30, 60 and 90 g/l) and starch (45, 60 and 75 g/l) as the first test and four types of nutrients with different levels: humic acid (6, 9 and 12 g/l), filter cake ( 5, 10 and 15 g/kg), superabsorbent (10, 30 and 45 g/kg) and microcombi fertilizer (10, 20 and 30 g/kg) as the second experiment with control in a completely randomized design with 3 replications In 2020, in Khuzestan-Iran sugarcane research station. The results showed that the effect of four types of binder on sugarcane plant characteristics such as germination rate, stem length, stem dry weight and plant moisture content was significant and the most positive effect was observed in starch gum (60 g/l). Also, four nutrients had a significant effect on some characteristics such as germination rate, stem length, stem dry weight and plant moisture content. The most positive effect for characteristics was observed in humic acid (12 g/l). Due to the most positive effect of starch gum, in later studies, this glue can be used as a binder, along with other nutrients and fillers for planting lateral buds of sugarcane stems to produce artificial single-seeded sugarcane seeds.