To investigate the effect of nitroxin and vermicompost on the qualitative and functional characteristics of blackberry, a factorial experiment was conducted in a completely randomized design with four replications in pots. The first factor was the percentage of vermicompost at five levels (0, 10, 20, 30 and 40) and the second factor was the level of inoculation and non-inoculation with nitroxin (Ni). The results showed that the treatments had a significant effect on the studied traits. The highest fruit weight, number of fruits, and yield was observed in 20% vermicompost. Maximum fruit length (1.62 cm) obtained in 10% vermicompost and nitroxin inoculation. The highest fruit diameter was obtained in 30% vermicompost. Also, in biochemical parameters, the application of 10% vermicompost produced the most soluble solids and flavor index. The highest amount of anthocyanin at 551 and 563 (mg/L fruit juice) was obtained respectably in 20 and 30% vermicompost treatments. Application of nitroxin along with vermicompost yielded 30% of the most vitamin C. In general, the results showed that the application of vermicompost at levels of 20 to 30% has the best effect on the quantitative and qualitative traits of blackberry and nitroxin can reduce the negative effect of high vermicompost and increase the effect of vermicompost on the blackberry plant.