Document Type : Research Paper


1 Ph.D. Candidate, Department of Biology, Payame Noor University, East Tehran Center, Tehran, Iran.

2 Professor, Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.

3 Assistant Professor, Department of Biology, Payame Noor University, East Tehran Center, Tehran, Iran.

4 Assistant Professor, Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.

5 Associate Professor, Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.


The purpose of the present study is to evaluate the effects of different levels of vermicompost on quantity and quality of Lilium longiflorum LA hybrid cv. Dynamix, in a completely randomized design with 5 treatments and 3 replications. The treatments include 0 (control), 20%, 35%, 50%, and 65% of vermicompost pot (V/V) in 70% cocopeat and 30% perlite of bed culture under greenhouse conditions. Once the buds’ color change from bold green to colorless green, cut flowers are removed from various treatments and the flower’s inflorescence is studied in the laboratory. Results show that fresh weight of Lilium in low-level vermicompost surpass the other treatments, especially the 20% one. Qualitative parameters such as chlorophyll, protein leaf, anthocyanin, and total soluble solid of the petal have had a significant increase in all vermicompost treatments, compared to the control. Overall result examination of this study indicates the 20% vermicompost treatment has been more suitable, thanks to its superior moisture preservation at plant shoots as well as the improvement of some morphologic traits like the number of flowers as well as addition of leaf area, which contributes to the cultivation of better quality Lilium LA hybrid flower.


Abou-Aly, H.E., & Mady, M.A. (2009). Complemented effect of humic acid and biofertilizers on wheat (Triticum aestivum L.) productivity. Annals of Agricultural Sciences. 47(1), 1-12.
Anderson, N.O. (2007). Flower Breeding and Genetics: Issues, Challenges and Opportunities for the 21st century. Springer, Netherlands.
Arancon, N.Q., Edwards, C.A, Babenko, A. Cannon, J.  Galvis, P., & Metzger, J.D. (2008). Influences of vermicomposts produced by earthworms and microorganisms from cattle manure, food waste and paper waste, on the germination, growth and flowering of petunias in the greenhouse. Applied Soil Ecology. 39, 91-99.
Arancon, N.Q., Edwards, C.A., Webster, K.A., & Buckerfield, J.C. (2011). The potential of vermicomposts as plant growth media for greenhouse crop production. In: Edwards, C.A., Arancon, N.Q., Sherman, R. (Eds.) Vermiculture Technology (pp. 103-128). CRC Press, Boca Raton, FL, USA.
Armitage, A.M., & Laushman, J.M. (2008). Specialty Cut Flowers: The Production of Annuals, Perennials, Bulbs and Woody Plants for Fresh and Dried Cut Flowers. (2nd ed.). Timber Press, USA.
Benschop, M., Kamenetsky, R., Le Nard, M., Okubo, H., & De Hertogh, A. (2010). The global flower bulb industry: Production, utilization, research. Horticulture Review, 36, 1-115.
Benvenuti, S., Bortolotti, E., & Maggini, R. (2016). Antioxidant power, anthocyanin content and organoleptic performance of edible flowers. Scientia Horticulturae. 199, 170-177.
Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analitical Biochemistry, 72, 284-254.
Canellas, L.P., Olivares, F.L., Okorokova-Façanha, A.L., & Façanha, A.R. (2002). Humic acids isolated from earthworm compost enhance root elongation, lateral root emergence, and plasma membrane H+-ATPase activity in maize roots. Plant physiology. 130(4), 1951-1957.
Datta, Sh., Singh, JA., Singh,  JO., Sharanpreet Singh, Sh., & Singh, SI. (2018). Assessment of genotoxic effects of pesticide and vermicompost treated soil with Allium cepa test. Sustainable Environment Research, 28, 171-178.
Edwards, C.A., & Arancon, N.Q. (2004). The use of earthworms in the breakdown of organic wastes to produce vermicomposts and animal feed protein. In: C.A. Edwards (Ed.), Earthworm Ecology (pp. 345-438). CRC Press, Boca Raton, FL, USA.
Fan, H.M., Wang, X.W., Sun., X., & Li, Y.Y. (2014). Effects of humic acid derived from sediments on growth, photosynthesis and chloroplast ultrastructure in chrysanthemum. Scientia Horticulturae, 177, 118-123.
Fan, H.M., Li, T., Sun, X., Sun, X.Z., & Zheng, C.S. (2015). Effects of humic acid derived from sediments on the postharvest vase life extension in cut chrysanthemum flowers. Postharvest Biology and Technology. 101, 82-87.
Gajalakshmi, S., & Abbasi S.A. (2002). Effect of the application of water hyacinth compost and vericompost on the growth and flowering of Crossandra undulaefolia and on several vegetables. Bioresource Technology. 85, 197-199.
Giusti, M.M., & Wrolstad, R.E. (2001). Characterization and measurement of anthocyanins by UV-visible spectroscopy. Current Protocols in Food Analytical Chemistry. 1, F1.2.1-F1.2.13.
Hidlago P.R., Matta, F.B., & Harkess R.L. (2006). Physical and chemical properties of substrates containing earthworm castings and effects on marigold growth. Horticulture Science, 41, 1474-1476.
Lichtenthaler, H.K., & Lester Packer, R.D. (1987). Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Method in Enzymology, 148, 350-382.
Mashahiri, H., & Hassanpour Asil, M. (2018). Evaluation of gibberellic acid and humic acid on morpho-physiological indices and vase life of cut flower daffodil (Narcissus pseudonarcissus). Journal of Plant Production Research, 24(4), 79-92. (In Persian)
Nardi, S.D., Pizzeghello, A., Muscolo, &. Vianello, A. (2002). Physiological effects of humic substances on higher plants. Soil Biochemistry. 34(11), 1527-1536.
Parandian, F., & Samavat, S. (2012). Effects of fulvic acid and humic acid on anthocyanin soluble sugar, α-amylase enzyme and some micro-nutriant elements in Lilium. International Research Journal of Applied and Basic Science. 3(5), 924-929.
Peterse, A. (2010). Dutch lily breeding: Past, present and future. Presentation at Lilytopia. Longwood Gardens, Kennett Square, PA, USA.
Quaggiotti, S., Ruperti, B., Pizzeghello, D., Francioso, O., Tugnoli, V., & Nardi, S. (2004). Effect of low molecular size humic substances on nitrate uptake and expression of genes involved in nitrate transport in maize (Zea mays L.). Journal of Experimental Botany. 55(398), 803-813.
Salman, S.R., Abou-hussein, S.D., Abdel-Mawgoud A.M.R., & El-Nemr, M.A. (2005). Fruit yield and quality of watermelon as affected by hybrids and humic acid application. Journal of Applied Sciences Research, 1(1), 51-58.
Seyedi, N., Mohammadi Torkashvand, A., & Allayari, M.S. (2013). Investigating effect of calcium concentration under hydroponic condition on quantitative and qualitative growth of Lilium. Journal of Ornamental and Horticultural Plants. 3(1), 19-24.
Subler, S., Edwards, C.A., &  Metzger, J. (1998). Comparing vermicomposts and composts. Biocycle, 39, 63-66.
Suthar, S. (2009). Impact of vermicompost and composted farmyard manure on growth and yield of garlic (Allium sativum L.) field crop. International Journal of Plant Production. 3(1), 27-38.
Tejada, M., & Gonzalez, J.L. (2003). Influence of foliar fertilization with amino acids and humic acids on productivity and quality of asparagus. Biological Agriculture and Horticulture, 21(3), 277-291.
van Doorm, W.G. (2004). Is petal senescence due to sugar starvation? Plant Physiology, 134, 35-42.
van Tuyl, J.M., & Arens, P.  (2011). Lilium: Breeding history of the modern cultivar assortment. Acta Horticulture, 900, 223-230.
Wu, S.C., Caob, Z.H., Lib, Z.G., Cheunga, K.C., & Wong, M.H. (2005). Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma. 125, 155-166.
Yadav, K.D., Tare, V., & Ahammed, M.M. (2012). Integrated composting–vermicomposting process for stabilization of human faecal slurry. Ecological Engineering, 47, 24-29.