Evaluation of Biochemical traits in some Pistachio Rootstocks under salt stress to select tolerant Rootstocks

Mohammadi, Khiermohammad; Ebadi, Ali; Askari Sarchishmeh, Mohammad Ali; Fattahi Moghaddam, Mohammad Reza; Hokmabadi, Hossein

doi:10.22059/jci.2019.257752.2021

Document Type : Research Paper

Authors

¹ Ph.D. Candidate, Department of Horticultural Science and Landscape Engineering, Faculty of Agricultural Science and Engineering, College of Agricultural and Natural Resources, University of Tehran, Karaj, Iran

² Professor, Department of Horticultural Science and Landscape Engineering, Faculty of Agricultural Science and Engineering, College of Agricultural and Natural Resources, University of Tehran, Karaj, Iran

³ Associate Professor, Department of Horticultural Science and Landscape Engineering, Faculty of Agricultural Science and Engineering, College of Agricultural and Natural Resources, University of Tehran, Karaj, Iran

⁴ Assistant Professor, Pistachio Research center, Agricultural Research Education and Extention Organization of Semnan Province, Dameghan center, (Iran).

https://doi.org/10.22059/jci.2019.257752.2021

Abstract

Plants employ antioxidant mechanism to face different stresses. In this research work the effect of salinity stress, caused by increasing sodium chloride, on the antioxidative system and some physiological characteristics of selected pistachio seedlings (Badami-Riz-e- Zarand, Qazvini, Sarakhs, Atlantica UCB1, and Hybrids of Pistacia. vera cv. Badami-Riz-e-Zarand × P. Integerrima, P. vera cv. Sharakhs× P. Integerrima, P. atlantica Desf. × P. Integerrima ) was studied. A greenhouse experiment was conducted as a factorial based on a completely randomized design with three replications in Cocopit and Perlite media. Treatments consisted of four salinity levels (0, 75, 150 and 225 mM NaCl) and above mentioned Root stocks. Using salt in nutrient solution was carried out in the second year of seedling growth for nine weeks. At the end of experiment, some physiological and biochemical traits of seedlings were measured. Results showed that, leaf area and total chlorophyll contents decreased in all treated plants. Under the salinity stress, a higher reduction in the observed traits was noticed in the Atlantica rootstock while less effect on the Badami-Riz-e-Zarand was noticed. Also, it has been found that by increasing salinity levels the levels of proline, total phenol content and antioxidant enzymes activity were higher in Badami-Riz-e-Zarand × P. Integerrima, Badami-Riz-Zarand and Qazvini compared to others. It seems that Hybrid of Badami-Riz-e-Zarand × P. Integerrima, Badami-Riz-Zarand and Qazvini rootstocks had more ability to sustain leaf water and proteins and therefore, were more resistant to salinity stress.

Keywords

References

Abbaspour, H. (2012). Effect of Salinity on Peroxidation of Lipids, Antioxidant Enzymes and Proline Accumulation in Pistachio Plants. Iranian Medicinal Plants Res, 6, 526-529. (In Persian)

Aebi, H. (1984). Catalase in vitro. Methods in enzymology, 105, 121-126.

Arzani, K., Ghasemi, M., Yadollahi, A. & Hokmabadi, H. (2013). Study of foliar epidermal anatomy of four pistachio rootstocks under water stress. Idesia (Aricaultur), 31, 101-107.

Ashraf, M. & Harris, P.J.C. (2005). Abiotic Stresses: Plant Resistance through Breeding and Molecular Approaches. Haworth Press, New York, USA, 54, 376-386.

Baqerzadeh, A., Kavousi, H., Khezri, M. & Mirzayee S. (2017). Study of protein expression pattern and some morphological and biochemical characteristics in pistachio roots of white almond and almonds in Zarand under salinity stress. Agricultural Biotechnology, 8, 16-32. (In Persian).

Bates, L. S., Waldren, R. P. & Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. plant soil and water relationship, 39, 205-207.

Bradford, M. M. (1976). A rapid and sensitive method for quantitaion of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.

Fletcher, R., Gilley, A., Davis, T. & Sankhla, N. (2000). Triazoles as plant growth regulators and stress protectants. Horticutual Research, 24, 55-138.

Gao, S., Ouyang, C., Wang, S., Xu, Y., Tang, L. & Chen, F. (2008). Effects of salt stress on growth, antioxidant enzyme and phenylalanine ammonia-lyae activities in Jatropha cursas L. seedlings. Plant soil and Environment, 54(9), 374-381.

Garaghanipur, N., Shiran, B., Khodambashie, M. & Molaie, A.R. (2014). Study of Proline accumulation and gene expression of P5CS in leaves and flower buds of common bean cultivars under drought stress. Journal of Agricultural Biotechnology, 6, 129-141.

Ghorbanli, M., Ahmadi, F., Manfard, A. & Bakhshi Khaniki Gh. (2010). The effect of salinity stress and its interaction with ascorbate on the activity of catalase, ascorbate peroxidase, proline and malondialdehyde in cumin (Cuminum cyminum L.) four weeks after germination. Iranian Journal of Medicinal and Aromatic Plant, 28, 14-27. (In Persian).

Jebara, S., Jebara, M., Limam, F. & Elarbi A, M. (2005). Changes in ascorbate peroxidase, catalase, guaiacol peroxidase and superoxide dismutase activities in common bean (Phaseolus vulgaris) nodules under salt stress. Plant Physiology, 162, 929-936.

Kamiab, F., Talaie, A., Khezri, M. & Javanshah, A. (2013). Exogenous application of free polyamines enhances salt tolerance of pistachio (Pistacia vera L.) seedlings. Plant Growth Regulation, 72, 257-268

Kar, M., & Mishra, D. (1976). Catalase, peroxidase, polyphenol oxidase activities during rice senescence. Plant Physiology, 57, 315-319.

Lawa, D. W. (1995). The effect of water deficit on photosynthesis. In: N. Smirnoff (ed) Envirnment and plant Metabobolism. 4,143-165.

Lotfi, N., Vahdati, K., Kholdebarin, B. & Najafian Ashrafi, E. (2009). Germination, mineral composition and ion uptake in walnut under salinity conditions. HortScience, 44(5),1352-1357.

Merati, M., Niknam, O., Hassanpour, H. & Mirmosomi M. (2014). Comparativ effects of salt stress on growth and antioxidative responses in different organs of Pennyroyal (Mentha pulegium L.). Journal of plant Research, 28, 1097-1107. (In Persian)

Meloni, D. A., Oliva, M. A., Martinez, C. A. & Cambraia. J. (2003). Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Brazilian Journal of Plant Physiology, 15, 12-21.

Mohajer Milani, P., Molahsini, H. & Nouri Hosseini S. (2004). Increasing the efficiency of irrigation with salt water in cotton and corn with one-dip leakage irrigation. Iran Soil and Water Science, 18, 36-39. (In Persian)

Nazar, R., Iqbal, N., Syeed, SA. & Khan, N. (2011). Salicylic acid alleviates decreases in photosynthesis under salt stress by enhancing nitrogen and sulfur assimilation and antioxidant metabolism differentially in two mungbean cultivar. Plant Physiology, 168, 807–815.

Panahi, B., Ismail Poor, B., farbod., F, Mozanpur Kermani, M. & Ferir mehan, H. (2002). Pistachios: Planting, keeping and harvesting (PP. 54). Agricultural Extension Education. (In Persian)

Pe rez-Pe´rez, J. G., Robles, J. M., Tovar, J. C. & Botia, P. (2009). Response to drought and salt stress of lemon ‘Fino 49’ under field conditions: Water relations, osmotic adjustment and gas exchange. Scientia Horticulturae, 122, 83–90.

Plewa, M. J., Smith, S. R. & Wagner, E. D. (1991). Diethyldithiocarbamate suppresses the plant activation of aromatic amines into mutagens by inhibiting tobacco cell peroxidase. Mutation Research Journal, 247, 57-64.

Radotic, K., Ducic, T. & Mutavdzic, D. (2000). Changes in perxidase activity and isoenzymes in spruce needles after exposure to different concentrations of cadmium. Environmental and Experimental Botany, 44, 105-113.

Rohman, A., Riyanto, S., Yuniarti, N., Saputra, W. R., Utami, R. & Mulatsih, W. (2010). Antioxidant activity, total phenolic, and total flavaonoid of extracts and fractions of red fruit (Pandanus conoideus Lam). Department of Pharmaceutical Chemistry Indonesia Gadjah Mada University International Food Research Journal, 17, 97-106.

Sohrabi, N., Tajabadipour, A., Motamed, N. & Seyedi, M. (2011). A change in leaves protein pattern of some pistachio cultivars under salinity condition. International Journal of Nuts and Related Sciences, 2, 67-74.

Journal of Crops Improvement

Evaluation of Biochemical traits in some Pistachio Rootstocks under salt stress to select tolerant Rootstocks

References

References

Volume 21, Issue 3
September 2019
Pages 323-336

Evaluation of Biochemical traits in some Pistachio Rootstocks under salt stress to select tolerant Rootstocks

References

References

Volume 21, Issue 3September 2019Pages 323-336

Volume 21, Issue 3
September 2019
Pages 323-336