Nooshin Derakhshan; Hossein Alaei; Rohollah Saberi Riseh; Ebrahim Sedaghati; Asghar Rahimi
Abstract
Salinity can prevent the uptake of soil mineral ions due to the negative effect of sodium ions and disturbance of ion balance, but the use of Trichoderma biocompounds improves growth under salinity stress. To investigate the effect of four salinity-resistant Trichoderma strains on growth traits and mineral ...
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Salinity can prevent the uptake of soil mineral ions due to the negative effect of sodium ions and disturbance of ion balance, but the use of Trichoderma biocompounds improves growth under salinity stress. To investigate the effect of four salinity-resistant Trichoderma strains on growth traits and mineral element uptake of eggplant seedlings under stress conditions, an experiment has been performed in a completely randomized factorial design with three replications in the greenhouse of Vali-e-Asr University of Rafsanjan during 2016-2017. The first factor is salinity in four levels including the control and concentrations of 8, 12, and 16 dS/m and the second factor is Trichoderma as T. aureoviride (T148-2, T189-4) and T. virens (T145, T133-1) as well as the control. The results show that under the influence of salinity, a significant decrease in growth traits takes place. At the highest salinity level, the average dry weight of roots and shoots is 50% and 72%, respectively. For stem diameter and seedling height, a 20% decrease is observed compared to the control. However, inoculation with Trichoderma improves these traits, resulting in a 1.6-fold increase in root dry weight (T142-8), a 2.7-fold increase in shoot dry weight (T133-1), and a 1.3-fold increase in seedling height (T189-4) in comparison to the control. The highest diameter increase could be observed in T142-8 strain by 38%. Using Trichoderma leads to significant changes in sugar, sodium, potassium, and calcium content. The results of this study show that Trichoderma can be a suitable biological agent to increase tolerance at high levels of salinity stress and improve the growth traits of eggplant seedlings.
Azizollah Khandan-Mirkohi; Naghmeh Alizadeh kordestani
Abstract
In this research, possibility of bulblet production from different cuttings including leaf, leaf-bud and leafy-stem of Lilium oriental hybrid cv. ‘Star Gazer’ evaluated under different levels of Indole-3-butyric acid (IBA) with 0, 250, 500, 750 and 1000 mg per liter (ppm). The cuttings were ...
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In this research, possibility of bulblet production from different cuttings including leaf, leaf-bud and leafy-stem of Lilium oriental hybrid cv. ‘Star Gazer’ evaluated under different levels of Indole-3-butyric acid (IBA) with 0, 250, 500, 750 and 1000 mg per liter (ppm). The cuttings were taken from stems which grown from cold treated bulbs in early October. A factorial experiment was done in Department of Horticulture Sciences, University College of Agriculture and Natural Resources, University of Tehran in 2014-2015, based on Randomized complete blocks design with three replications and nine cuttings per replicate. The results showed that rooting percentage and leaf number decreased on leaf cuttings by increasing auxin concentration, while these traits increased on leaf-bud and leafy stem cuttings. In contrast to the results in the rooting and leaf number traits, number of bulblets rose in leaf cuttings from 14 in control to 21, in leaf-bud cuttings from three to 20 and in leafy stem cuttings from one to 16 in IBA levels of 250, 500 and 750 ppm, respectively. Exceeding IBA concentration had reverse effect on bulblet number, correspondingly. Even so, leaf number and rooting percentage declined on leaf cuttings by application of IBA, but it was useful for production of more bulblets on leaf, leaf-bud and leafy-stem cuttings in IBA levels of 250, 500 and 750 ppm, respectively.
Maryam Tatari; Seyed Asghar Mousavi
Abstract
According to problems caused by application of non-uniform seedling rootstocks in stone fruit treesorchards, the use of uniform and consistent rootstocks with these trees is essential. For determination ofthe best culture medium, explants of apical and lateral buds of Tetra, Nemaguard and GF677 rootstockswere ...
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According to problems caused by application of non-uniform seedling rootstocks in stone fruit treesorchards, the use of uniform and consistent rootstocks with these trees is essential. For determination ofthe best culture medium, explants of apical and lateral buds of Tetra, Nemaguard and GF677 rootstockswere prepared. Explants were placed in modified MS, WPM and Knop media each containing 0.6 mg/lBAP and 0.01mg/l NAA in mid-Spring and after disinfection. This research was carried out as factorialexperiment in a completely randomized design with four replicates and four observations per eachreplication. Tetra, GF677 and Nemaguard rootstocks produced the highest of plantlet numberrespectively. Nemaguard rootstock did not show any proliferation in Knop medium and Nemaguardexplants showed chlorosis on this medium. This medium caused chlorosis in produced plantlets of Tetraexplants. Explants of every three rootstocks were produced the highest plantlet number and length in MSmodified medium. Therefore, combinations of plant growth regulators were evaluated in this medium.Three different combinations were evaluated. Among different combinations of plant growth regulators(0.8 and 1 mg/l BAP and 0.1 and 0.5 mg/l NAA), the highest plantlet number and length were producedin MS medium containing 0.6mg/l BAP and 0.01mg/l NAA. Increase of plant growth regulatorsconcentrations led to vitrification and callus production in GF677 rootstock. The highest root number androoting percent were achieved in modified MS medium containing 1 mg/l NAA and explants of Tetrarootstock produced the highest root number and length.
zeinab karamad; Ebrahim Ganji Moghadam; ahmadreza bolandi
Abstract
This study was conducted with the main prupose of investigation the effects of culture medium and the concentration of growth regulators on proliferation rate, rooting and the acclimatization of Gisela 6 rootstock at Khorasan Razavi Agriculture and Natural Resource Research Center during 2011-2012. In ...
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This study was conducted with the main prupose of investigation the effects of culture medium and the concentration of growth regulators on proliferation rate, rooting and the acclimatization of Gisela 6 rootstock at Khorasan Razavi Agriculture and Natural Resource Research Center during 2011-2012. In this experiment, the proliferation and rooting were performed in six kinds of culture medium (DKW, WPM and MS in two solid and liquid media) supplemented with plant growth regulators included TDZ and BAP in zero, one, two mg/l plus 0.01 mg/l IBA in all treatments of the proliferation and IBA with zero, one, two, three mg/l in rooting step. The results showed that the highest number and length of shoot were 6.48 shoot and 3.14 cm, respectively in solid WPM medium and two mg/l BAP concentration and liquid MS medium with one mg/l concentration of TDZ. The liquid MS medium in two mg/l of IBA caused the highest percentage of rooting and root length, 93.90 percent in 11.76 cm, respectively. Although applying the liquid media resulted in better acclimatization of plantlets, but the highest percent of plantlets survival was obtained in WPM liquid medium with two mg/l concentration of IBA at about 91.80 percent.