Document Type : Research Paper


1 M.Sc. Student, Department of Horticulture, Islamic Azad University, Shirvan Branch, Islamic Azad University, Shirvan, Iran

2 Assistant Professor, Department of Horticulture, Khorasan Razavi Natural Resource and Agricultural Research Center, Mashhad, Iran

3 Assistant Professor, Department of Seed and Plant Improvement of Khorasan Razavi Natural Resource and Agricultural Research Center, Mashhad, Iran


This study was conducted with the main prupose of investigation the effects of culture medium and the concentration of growth regulators on proliferation rate, rooting and the acclimatization of Gisela 6 rootstock at Khorasan Razavi Agriculture and Natural Resource Research Center during 2011-2012. In this experiment, the proliferation and rooting were performed in six kinds of culture medium (DKW, WPM and MS in two solid and liquid media) supplemented with plant growth regulators included TDZ and BAP in zero, one, two mg/l plus 0.01 mg/l IBA in all treatments of the proliferation and IBA with zero, one, two, three mg/l in rooting step. The results showed that the highest number and length of shoot were 6.48 shoot and 3.14 cm, respectively in solid WPM medium and two mg/l BAP concentration and liquid MS medium with one mg/l concentration of TDZ. The liquid MS medium in two mg/l of IBA caused the highest percentage of rooting and root length, 93.90 percent in 11.76 cm, respectively. Although applying the liquid media resulted in better acclimatization of plantlets, but the highest percent of plantlets survival was obtained in WPM liquid medium with two mg/l concentration of IBA at about 91.80 percent.


1 . Akita M, Negishi K, Kitano A, Wasaki M, Ohta Y, Kuriu T and Takii T (2006) Mass propagation of cherry (Prunus cerasus × Yedonsis matusum) through shoot primordial. Acta Horticulturae. 725: 579-584.
2 . Alkan K, Cetiner S, Aka Y, Yalcin MY and Kuden AB (1997) In vitro multiplication of clonal apple rootstocks M9, M26 and MM 1 06 by meristem culture. Acta Horticulturae. 441: 325-327.
3 . Al-sabagh M, Abdul-Kader A, Khoder M and Abdul-Rahman A (1999) In vitro propagation of semi-dwarf cherry rootstock. Plant Cell, Tissue and Organ Culture. 59(3): 203-208.
4 . Andersen RL, Robinson T and Lang GA (1999) Managing the Gisela cherry rootstocks. New York Fruit Quarterly. 7(4): 1-4.
5 . Baghwat B and Lane D (2004) In vitro shoot regeneration from leaves of sweet cherry (Prunus avium) ‘Lapins’ and ‘Sweet heart’. Plant Cell, Tissue and Organ Culture. 78(2): 173-181.
6 . Buzkan N, Cetiner S, Yalcin-Mendi Y and Di Terlizzi B (1997) Clonal propagation of disease free rootstooks for sour and sweet cherry by meristem culture. Acta Horticulturae. 441: 327-332.
7 . Channuntapipat ChM, Sedgley and Collins G (2003) Microporopagation of almond cultivars Nonpariel and Ne plus Ultra and the hybrid rootstocks Titan Nemagard. HortSciense. 98: 473-484. 
8 . Daneshvar Hossini A, Ganji Moghadam E and Anahid S (2010) Effects of Media Cultures and Plant Growth Regulators in Micro Propagation of Gisela 6 Rootstock. Annals of Biological Research. 1(2):135-141.
9 . Dorkovic J (2006) Rapid micropropagation of maturawild cherry (Prunus avium L.). Biologia Plantarum. 50(4): 733-736.
10 . Driver JA and Kuniyuki AH (1984) In vitro propagation of paradox walnut rootstock. HortScience. 19(4): 507-509.
11 . Erbenova M, Paprstein F and Seldak J (2001) In vitro propagation of dwarf rootstocks for sweet cherry. Acta Horticulturae. 560: 477-480.
12 . Hatchinsun FJ and Zimmerman HR (1987) Tissue culture of temperate fruit and nut trees. In: J. Janick (ed). Horticultural Reviews. Vol. 9. Van Nostrand Reinhild Company Inc. Pp. 337-349.
13 . Hossain SN, Munshi MR, Islam MR, Hakim L and Hossain M (2003) In vitro propagation of plum (Zyziphus jujubalam). Plant Tissue Culture. 13(1): 81-84.
14 . Matt A and Johannnes AJ (2005) In vitro plant regeneration from leaves and internodes sections of sweet cherry cultivars (Prunus avium L.). Plant Cell Reports. 24(8): 468-476.
15 . Mc Cown B and Lioyd GB (1980) Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot tip culture vitro cultue. Proceedings of the international plant propagation society. 30: 421-427.
16 . Muna AS, Ahmad AK, Mahmoud K and Abdul-Rahman K (1999) In vitro propagation of a semi-dwarfing cherry rootstock. Plant Cell, Tissue and Organ Culture. 59: 203-208.
17 . Murashig T and Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue culture. Physiologia Plantarum. 15: 473-497.
18 . Perez-Tornero O, Burgos L, Egea I and Lopez JM (2000) Apricot meristem tip culture. Acta Horticulturae. 488: 417
19 . Perez-Tornero O and Burgos L (2000) Different media requirements for micropropagation of apricot cultivars. Plant Cell Tissue and Organ Culture. 63: 133-141.
20 . Perez-Tornero O, Egea J, Vanoostende A and Burgos L (2000) Assessment of factors affecting adventitious shoot regeneration from in vitro cultured leaves of apricot. Plant Science. 158: 61-70.
21 . Perez-Tornero O, Lopez JM, Egea J and Burgos L (2000) Effect of basal media and growth regulators on the in vitro propagation of apricot (Prunus armeniaca L.) cv. Cannino. Journal of Horticultural Science and Biotechnology. 75:283-286.
22 . Prushki K, Astatkie T and Nowak J (2005) Tissue culture propagation of Mongolian Cherry (Prunus fruticosa) and Nanking Cherry (Prunus tomentosa). Plant Cell Tissue and Organ Culture. 82(2): 207-211.
23 . Quoirin M and Lepoivre P (1977) Improved medium for in vitro culture of Prunus sp. Acta Horticulturae. 78: 437-442.
24 . Rogaleski M, Moraeas LK, Feslibino C, Crestani L, Guerra MP and Silva AL (2003) Acclimazation of micropropagated Prunus sp. Revista Brasileira de Fruticultura. 25(2): 279-281.
25 . Ruzic D, Saric M, Cerovic R and Culafic L (2000) Relationship between the concentration of macro elements, their uptake and multiplication of cherry rootstock Gisela 5 in vitro. Plant Cell Tissue and Organ Culture. 63(1): 9-14.
26 . Ruzic D, Saric M, Cerovic R and Culafic L (2001) Change in macroelement content of the media and in sweet cherry Inmil GM 9 shoots during in vitro culture. Journal of Horticultural Science and Biotechnology. 78: 295-299.
27 . Ruzic DV and Vajovic TI (2008) The effect of cytokenins types and their concentration on in vitro multiplication of sweet cherry C.V. Lapins (Prunus avium L.). HortScience. 35(1): 12-21.
28 . Sarropoulou V, Dimassi-Theriou K and Therios I (2013) Indole-3-butyric acid and myo-inositol impacts on in vitro rooting of the cherry rootstocks CAB-6P and Gisela 6. Biologia Plantarum. 57(4): 613-619.
29 . Sedlak J and Paprstein F (2008) In vitro shoot proliferation of sweet cherry cultivars Karesova and Rivan. HortScience. 35(3): 95-98.
30 . Sisko M (2011) In vitro propagation of Gisela 5 (Prunus cerasus × P. canescens). Agricultura 8: 31­34.
31 . Tang M, Pen ZL and Krczal G (2002) Plant regeneration from leaves of sweet and sour cherry cultivars. Scientia Horticulturae. 93(3): 235-244.