safiye arab; Mehdi Baradaran Firouzabadi; Ahmad Gholami; mostafa haydari
Abstract
In order to investigate the effect of ellagic acid on the growth indices and leaf aging process in soybean, an experiment was conducted during the two years of 2018 and 2019 in the research farm of Shahrood University of Technology's Faculty of Agriculture. The experiment was carried out factorial in ...
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In order to investigate the effect of ellagic acid on the growth indices and leaf aging process in soybean, an experiment was conducted during the two years of 2018 and 2019 in the research farm of Shahrood University of Technology's Faculty of Agriculture. The experiment was carried out factorial in the form of a randomized complete block design in three replications. The experimental treatments include the initial seed quality at two levels (normal seeds and aged seeds) and the application of ellagic acid at four levels (control, priming, foliar spraying and combined application of priming and foliar spraying with a concentration of 50 mg/L). The results showed that accelerated aging decreased the amount of chlorophyll, carotenoid, plant height, leaf and stem dry weight, oil percentage and seed yield compared to the control. The use of ellagic acid in the form of priming, foliar spraying and their combination in this study increased the percentage of oil by 0.23, 0.24 and 0.29%, respectively, compared to the control. Priming, foliar spraying and the combined application of priming and foliar spraying with ellagic acid improved the grain yield by 23.59, 23.23 and 55.48%, respectively. The use of ellagic acid as seed priming and foliar spraying better than other levels. Finally, it is recommended to use this material as a priming to increase the percentage of oil and yield of soybean.
farzaneh Pimard; Abdolrahman Mohammadkhani; Ali Niazi; Alireza Shahsavar; Mohammad-Reza Nouri-Emamzadei
Abstract
Drought stress is one of the limiting factors of agriculture in many parts of the world, especially Iran. Understanding the mechanism behind drought stress’ effect on physiological and biochemical processes of genotypes is very useful for selecting and breeding genotypes compatible with Iranian ...
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Drought stress is one of the limiting factors of agriculture in many parts of the world, especially Iran. Understanding the mechanism behind drought stress’ effect on physiological and biochemical processes of genotypes is very useful for selecting and breeding genotypes compatible with Iranian conditions. For this purpose, the present study has been conducted in 2018-2019 in the Biotechnology Research Institute of Shiraz University to investigate the effect of drought stress on physiological and biochemical characteristics of both years’ old pomegranate seedlings of Wonderful cultivar in greenhouse conditions. Irrigation has been carried out at four levels of 100% (control), 75%, 55%, and 35% of field capacity for 50 days. The experiment is based on a completely randomized design with three replications. The results show that drought stress has significantly increased carotenoids, flavonoids, malondialdehyde, and proline. The relative leaf water content, cell membrane stability and anthocyanins has decreased, though there has been no significant difference in chlorophyll and glycine betaine levels between drought treatments. Also, the hydrogen peroxide (81%) and activity of superoxide dismutase (480%), catalase (96%), and ascorbate peroxidase (96%) in 35% of field capacity significantly has increased. According to the results of this study, especially the increase in proline and antioxidant enzymes under drought stress, tolerance mechanisms in pomegranate cultivar Wonderful can be associated with active osmotic regulation and active enzymatic antioxidant system.
Mahmood Moradi Chegeni; Ahmad Ali Shoushi Dezfuli; Mahmood Tohidi; Mehdi Sadeghi; Farbod Fotouhi
Abstract
One of the important activities to achieve drought tolerant cultivars is screening and selection of cultivars, based on yield and biochemical characteristics. A split-plot experiment arranged in a randomized complete blocks design with three replications has been carried out in Safiabad Agricultural ...
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One of the important activities to achieve drought tolerant cultivars is screening and selection of cultivars, based on yield and biochemical characteristics. A split-plot experiment arranged in a randomized complete blocks design with three replications has been carried out in Safiabad Agricultural Research Center during two growing seasons (2017-19). The main plots are consisted of different irrigation regimes based on crop’s water requirement (25%, 50%, 75%, and 100%) and sub plots include five levels of alfalfa cultivars (Baghdadi, Nikshahri, Yazdi, Omid, and american Mesa-Sirsa). Results show that drought stress due to irrigation regimes have resulted in a significant reduction of forage dry matter yield and considerable changes of soluble sugar, glutation peroxidase, and catalase in alfalfa leaves. However, it has not greatly affected total protein. For forage dry matter yield, the highest statistical class belongs to Baghdadi cultivar under 100% water requirement supply treatment (2.824 t.ha-1). The lowest statistical class for forage dry matter yield has been observed in Yazdi and Mesa-Sirsa cultivars under 25% water requirement supply treatment (0.598 and 0.546 t.ha-1), respectively. Among the cultivars, examined in this experiment, Yazdi has been identified as a drought tolerant cultivar due to the stability of dry forage yield as well as high leaf antioxidants content.
Shahram Taheri; Ahmad Gholami; Hamid Abbasdokht; Hassan Makarian
Abstract
In order to evaluate the effects of seed priming to reduce water deficit stress in safflower cultivars, an experiment was conducted as a split plot factorial based on randomized complete block design with three replications at Shahrood Agricultural Research Center in 2015. The main plot consisted of ...
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In order to evaluate the effects of seed priming to reduce water deficit stress in safflower cultivars, an experiment was conducted as a split plot factorial based on randomized complete block design with three replications at Shahrood Agricultural Research Center in 2015. The main plot consisted of irrigation at three levels based on the evaporation from class A evaporation pan: non water deficit stress (60 mm evaporation), mild water deficit stress (120 mm evaporation) and severe water deficit stress (180 mm evaporation) and subplots consisted of two factors include safflower cultivars (Goldasht, Sina and Soffeh) and seed priming (Primed seeds with salicylic acid and non-primed). The results showed that severe water stress reduced the grain yield by about 29 percent compared to non stress conditions. In these conditions activity of superoxide dismutase, peroxidase, ascorbate peroxidase and catalase enzymes was increased by about 33, 25, 29 and 40 percent respectively. In severe water deficit conditions, content of malondialdehyde, proline and carotenoid significantly increased but the amount of chlorophyll was reduced. Priming of seeds with salicylic acid caused the antioxidant defense system activity to be increased by about 7-9 percent, therefore increased resistance of safflower plants to water stress and resulted in greater seed yield under water stress conditions. Interaction of irrigation and cultivar appeared to be significant on seed yield and activity of catalase, peroxidase and ascorbate peroxidase enzymes.
Ali Asghar Ghaderi; Barat Ali Fakheri; Nafiseh Mahdi Nezhad
Abstract
In order to investigate the effects of foliar application of ascorbic acid on the growth indexes and physiological traitsof thyme under drought stress, an experiment was conducted in the split plots based on randomized complete block design with three replications at the Research Farm, Faculty of Agriculture ...
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In order to investigate the effects of foliar application of ascorbic acid on the growth indexes and physiological traitsof thyme under drought stress, an experiment was conducted in the split plots based on randomized complete block design with three replications at the Research Farm, Faculty of Agriculture University of Zabol, Iran. The main factor was drought stress and applied based on the irrigation at 75, 55 and 35% FC and the subplot was foliar application with three levels including distilled water (control), 10 mM and 20 mM ascorbic acid. Main effects of drought stress and foliar application of ascorbic acid were significant (P≤0.01) for all studied traits. The interaction of water stress and foliar application of ascorbic acid was significant only for proline. Applying severe stress compared with control was decreased the total chlorophyll, carotenoid, root and shoot dry weight (29.0, 39.9, 50.5 and 43.0%, respectively), while the leaf proline and the root length were increased (44.2 and 26.6%, respectively). The foliar application of ascorbic acid (20 mM) significantly increased the amounts of photosynthetic pigments, shoot length, root and shoot weight. Simple correlation coefficients of the traits showed a significant and positive relationship between shoot dry weight and other traits, in drought stress and 20 mM foliar application of ascorbic acid conditions. Factor analysis was identified four factors for normal and four factors for severe stress conditions that at overall were explained 98 and 95% of total variation, respectively. In general, it was concluded that photosynthetic pigments and root-related traits would be the important yield related criteria (shoot dry weight), that can be beneficial in the development of thyme with higher performance under stress conditions.
Abbas Alizade ahmad abadi1; sarah khorasaninejad; Khodayar Hemmati
Abstract
In order to study the effects of Humic acid levels and irrigation regimes on the some morphological and biochemical characteristics of Echinacea purpurea L., a pot experiment was conducted as factorial based on randomized complete block design with three replications and four experimental units at research ...
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In order to study the effects of Humic acid levels and irrigation regimes on the some morphological and biochemical characteristics of Echinacea purpurea L., a pot experiment was conducted as factorial based on randomized complete block design with three replications and four experimental units at research farm of Gorgan University of Agricultural Sciences and Natural Resources, during growing season of 2014- 15. Treatments were four levels of irrigation regimes (40, 60, 80 and 100 percent field capacity) and humic acid including four levels: (0, 250, 500 and 1000 mg/kg). The results showed that irrigation regimes had significant effect on all measured properties except root length. Humic acid increased all of the growth caractristics and morphological traits in 5% level statistically, while had significant effect on total flavonoid and increased it. Interaction effect of humic acid and irrigation regimes was significant for wet and dry weight of root and shoot, total phenol and flavonoid. Therfore, the limited irrigation had negative effect on growth and plants increased their phenol and antioxidant components to stress coping. Humic acid increased growth and yield in plants that were faced with stress.
Sonia Jamali; Vali Rabiei; Javad Fattahi Moghadam
Abstract
Treated with different coating and application of methyl salicylate and salicylic acid are used for reduce chilling injury and maintain the quality of citrus fruit during storage. This experiment was carried out Citrus Research Institute of Ramsar in 91 years in order to study enzyme activity that involved ...
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Treated with different coating and application of methyl salicylate and salicylic acid are used for reduce chilling injury and maintain the quality of citrus fruit during storage. This experiment was carried out Citrus Research Institute of Ramsar in 91 years in order to study enzyme activity that involved in reducing chilling injury and Maintain the fruit quality based on Split Plot design with three replications. Fruits was treated and then placed in the cool storage at 5°C and 95 percent relative humidity)RH( for 80 days. Each 20 days with sampling investigated fruit enzyme activity was measured. Results showed the highest PAL enzyme activity was in fruits treated with methyl salicylate and the lowest was in fruits coated with the Bretix wax. The highest SOD enzyme activity related to Moro fruits which treated with methyl salicylate is in the first 20 days of storage )23/19 umg/FW(. The APX enzyme activity increased in both cultivars Thomson and Moro during storage. Relatively, treatments caused the change in APx, SOD and PAl enzyme activity.
Reza Fifaei; Reza Fotouhi Ghazvini; Behrooz Golein; Yusef Hamidoghli
Abstract
Citrus often were encountered with periodic droughts. For this reason, drought effect on six-month-old seedlings of Poncirus (PT) (Poncirus trifoliata Raf.),Troyer citrange (TC) (Citrus sinensis Osbec. × Poncirus trifoliata Raf.),Citrumello (CR) (Citrus paradisi M. × Citrus sinensis Osbec.),Sour ...
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Citrus often were encountered with periodic droughts. For this reason, drought effect on six-month-old seedlings of Poncirus (PT) (Poncirus trifoliata Raf.),Troyer citrange (TC) (Citrus sinensis Osbec. × Poncirus trifoliata Raf.),Citrumello (CR) (Citrus paradisi M. × Citrus sinensis Osbec.),Sour orange (SO) (Citrus aurantium L.),andCleopatra mandarin (CM) (Citrus reshni L.) were tested on 2014 in Ramsar Citrus Research Institute. This research was conducted as factorial experiment based on completely randomized design with three replications. The factors included commercial Citrus rootstocks and two level of irrigation. The results showed that maximum quantities of proline, total soluble sugars, malondialdehyde and carotenoid and minimum quantities of total, b and a chlorophyll were observed in drought stress. Maximum accumulation of proline (ordered 306.41 and 281.65 µmol/gdw) and total soluble sugars (ordered 233.79 and 137.9 mg/gdw) and minimum accumulation of malondialdehyde (ordered 179.21 and 204.1 µmol/gdw) were observed in SO and CM rootstocks under drought stress. Decreasing amount of total and chlorophyll a in CM and chl b in SO rootstock was more than other rootstocks and decreasing amount of total and b, a chlorophyll in CR was least. Furthermore, increasing amount of carotenoid in SO and CM was most and the least amounts in PT was observed. On the basis of the study, trifoliate rootstocks as Poncirus, Troyer citrange and Citrumello tolerate as much as drought.
mahdi mohammad zan=mani; vali rabiei; Mohammad Ali Nejatian; Mehdi Taheri
Abstract
Proline and glycine betaine are the most common compatible solutes that prevent plants from destructive effects of osmotic stresses by osmotic adjustment and protection of membranes, proteins and enzymes. It has been reported that exogenous application of these compounds, can increase plant resistance ...
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Proline and glycine betaine are the most common compatible solutes that prevent plants from destructive effects of osmotic stresses by osmotic adjustment and protection of membranes, proteins and enzymes. It has been reported that exogenous application of these compounds, can increase plant resistance to drought stress. Therefore a factorial experiment based on randomized complete block design with four replications was performed in four grapevine cultivars (‘Khushnav’, ‘Peykani’, ‘Perlette’, and ‘Flame Seedless’). Grapevines were irrigated by 70 percent of the vine water requirement, in drought stress treatment. Proline (10 mM) and glycine betaine (15 mM) were sprayed on grapevines at four growth stages (before flowering, flowering, sour cluster and veraison). Results showed that endogenous proline and amount of glycine betaine, peroxidase and ascorbate peroxidase enzyme activity in treated vines were significantly higher than the control, in both proline and glycine betaine treatments. Among of the treated vines, the most and the least amount of proline was measured in ‘Flame Seedless’ and ‘Peykani’, respectively. Other measured traits in cultivars did not show significant difference. According to the role of these enzymes in eliminating reactive oxygen spices (ROS) and also the role of proline and glycine betaine in antioxidants activating, using of these two osmolytes will be able to increase the vines tolerance to drought stress conditions.