Ehsan Hasanvand; Mostafa Darvishnia; Hosein Mirzaei najafgholi; Samira Pakbaz
Abstract
Objective: Every year, wheat is affected by various fungal diseases, including Fusarium blight. Seed inoculation with native PGPR is a suitable approach in health management, improving productions and their quality. Methods: To evaluate the bioinhibition ability and activity of antioxidant enzymes peroxidase ...
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Objective: Every year, wheat is affected by various fungal diseases, including Fusarium blight. Seed inoculation with native PGPR is a suitable approach in health management, improving productions and their quality. Methods: To evaluate the bioinhibition ability and activity of antioxidant enzymes peroxidase and catalase by endophytic bacteria in resistant and sensitive genotypes of wheat infected with FHB, a factorial experiment in the form of a completely randomized design was conducted in spring, 2022 at the Faculty of Agriculture of Lorestan University. The experimental treatments included four strains of Pseudomonas brassicacearum, Pseudomonas sp., Exiguobacterium sp., Acinetobacter calcoaceticus and their combination.Results: Results showed that among the four isolates the highest percentage of growth inhibition of fungi was related to the P. brassicacearum isolate with a value of 49.33%. The activity of antioxidant enzymes both in the infected resistant and susceptible cultivars at different times after inoculation with fungus increased significantly compared to the control. The combined treatment of bacteria+ resistant cultivar+ infected showed the highest average activity of catalase with an average of 12.41 and the treatment B3+ sensitive cultivar+ control showed the lowest average catalase activity with an average of 3.59. Also, the combined treatment of bacteria+ resistant cultivar+ infected showed the highest average of 1.93 and the B3+ sensitive cultivar+ control treatment showed the lowest average of peroxidase enzyme activity with an average of 0.58.Conclusion: Considering the high efficiency of the bacterial strains used in increasing the activity antioxidant enzymes and also their antifungal activity, they can be considered as a suitable option in producing healthy plants.
musa Ebrahimpour; Pourang Kasraie; Hamidreza Larijani; Hamidreza Tohidi moghadam; Sahar Honarmand Jahromi
Abstract
Objective: The purpose of this experiment was to investigate the effect of foliar spraying of bacterial herbicides and aqueous extracts of plants on the morphological characteristics and starch of barnyard grass weed and rice.Methods: This experiment was conducted as split factorial based on a randomized ...
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Objective: The purpose of this experiment was to investigate the effect of foliar spraying of bacterial herbicides and aqueous extracts of plants on the morphological characteristics and starch of barnyard grass weed and rice.Methods: This experiment was conducted as split factorial based on a randomized complete block design cityAmol with three replications in the two cropping years of 2018 and 2019. Factors included the foliar application of aqueous extracts plants and surfactant of sorghum, broccoli, nettle, eucalyptus, elder-berry, Streptomyces sp-albos containing thaxtomin, Xanthomonas campestris bacteria and control as the main factor, the concentration of the extract was zero, 5, 10 and 15 per thousand as a subfactor and the plant species of rice were Tarom cultivar and barnyard grass weed as a sub-sub factor.Results: By extracts foliar application, barnyard grass seed yield and biological yield decreased and the seed starch percent increased. Rice seed yield in the use of 5 per thousand broccoli extract and 5 per thousand Streptomyces sp-albos was not significantly different from the control. By increasing the concentration of the extract, seed yield and biological yield of rice and barnyard grass showed a further decrease. The lowest seed yield in rice at 3.16 tons/ha was related to elder-berry extract and in barnyard grass 0.68 and 0.61 tons/ha was related to nettle and elder-berry extract.Conclusion: According to the obtained results, Allelopathy and plant pathogens can be one of the basic ways to control weeds in a sustainable agricultural system.
Razieh Assadi; Mostafa Darvishnia; Mehdi Nasr Esfahani; Eidy Bazgir; Samira Pakbaz
Abstract
One of the most important pathogens in plants such as pomegranate, are root-knot nematodes (Meloidogyne spp.) which cause considerable damage to this important export product. Due to the importance of this subject, an study has been conducted between 2018 and 2019 in the Faculty of Agriculture of Lorestan ...
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One of the most important pathogens in plants such as pomegranate, are root-knot nematodes (Meloidogyne spp.) which cause considerable damage to this important export product. Due to the importance of this subject, an study has been conducted between 2018 and 2019 in the Faculty of Agriculture of Lorestan University. The treatments include organic fertilizer (40, 60, and 80 t/ha.), vermicompost (40 t/ha.) and Marygld product (200, 300 and 400 g/m2) with the study, itself, being in a completely random design on the dominant root-knot nematodes species, M. javanica under greenhouse conditions on pomegranate seedlings. The measurement indicators include gall index, the number of egg sacs, and the final population of egg and J2 larvae in the soil and the roots, as well as growth factors of pomegranate seedlings. Reproduction factor and the percentage of increase/decrease of root knot nematode population, have been calculated in each treatment compared to the initial population of the same treatment. Results show vermicompost (40 t/ha.) with 94.35% reduction in population of egg and larvae has had the highest effect on reducing the nematode population compared to other treatments. Marygld 400, 300, and 200 g/m2 treatments are ranked next, with a reduction of 93.47%, 91.21%, and 92.65% in nematode population, respectively followed by by nematode reduction of the composts of 80, 60, and 40 t/ha by 89.67%, 86.55%, and 86.81% respectively.
Mostafa Darvishnia; Moslem Moosavian
Abstract
The current study been conducted to achieveto effective antagonists for diseases control of seedling damping-off greenhouse pepper, casead by Rhizoctonia solani. Experiments has conducted based on completely randomized design in the faculty of agriculture and natural resources of Lorestan University ...
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The current study been conducted to achieveto effective antagonists for diseases control of seedling damping-off greenhouse pepper, casead by Rhizoctonia solani. Experiments has conducted based on completely randomized design in the faculty of agriculture and natural resources of Lorestan University in 2016-17. First, the fungi and biological agents were isolated and identified of the rhizosphere of Solanaceae plants. The antagonistic ability of fungal and bacterial isolates, such as Trichoderma harzianum and Trichoderma virens fungi and Bacillus subtilis bacteria, against R. solani was studied by using dual culture method. As well as antagonistic ability of fungal and bacterial agents and Trichomix HV biological fungicide were study in a greenhouse condition. The results showed that both species of T. harzianum and T. virens the prevented growth of fungi 9.45 and 15.59 percent respectively by colonization and sporulation in PDA. Antagonistic bacterial also caused effects such as outpour of the contents of hyphae, color change at the tip of hyphae, thinning and reduction of mycelium up to 8.47 percent. Also, results in greenhouse condition showed that B. subtilis and T. harzianum treatments have the most positive effects on growth traits such as height and weight (wet and dry) of the root and stem and reduced the severity of the disease up to 60.33 and 70.33 respectively; therefore they are the best antagonistic agents to control R. solani on pepper.