Document Type : Research Paper

Authors

1 Corresponding Author, Crop and Horticultural Science Research Department, Zanjan Agricultural and Natural Resources Research Center, AREEO, Zanjan, Iran. E-mail: h_nazari@ut.ac.ir

2 Cotton and Fiber Crop Research Department, Agricultural Research, Education and Extension Organization (AREEO), Varamin, Iran. E-mail: a.arefi@areeo.ac.ir

Abstract

Objective: In order to improve the tolerance of German chamomile cultivars to moisture regimes, this factorial experiment was conducted in a randomized complete block design in three replicates, in the agricultural college of Tehran university in 2013-14 cropping season.
Methods: Experimental treatments included moisture regimes (control and severe stress, i.e., 13% and 57% of allowable soil moisture depletion, respectively), two cultivars of chamomile (German Badgold and Hungarian modified cultivar) and foliar application with putrescine (with a concentration of 0.1 mM and 45 days after transfer to the pots).
Results: The results showed that the simple effect of putrescine polyamine on plant height and biomass was significant at 1% level and on harvest index at 5% level. Plant height did not have a significant response to putrescine in control conditions, but in severe stress (57% of soil water depletion), the effect of foliar application showed a significant boost (13.5%). The simple effect of putrescine on percentage and essential oil yield (at 1% level) was significant. Also, the interaction effect of moisture regimes and foliar application with putrescine on essential oil content (at 5% level) and essential oil yield (at 1% level) was considerable. Under optimal moisture conditions (13% of soil water depletion), putrescine resulted in a significant increase in essential oil content by 38%. But its foliar application in drought stress increased the essential oil content by only 4%. Foliar application of putrescine reduced the amount of farnesene and bisabolol B oxide in both conditions. Another important compound was bisabolone oxide A, wherein putrescine treatment had a positive effect on its accumulation so that under favorable conditions of moisture its concentration increased by 87%, but under conditions of stress its effect decreased, showing only 35%.
Conclusion: The use of putresin in chamomile is economically justified due to increased tolerance to dehydration stress and increased target secondary metabolites.

Keywords

اکبرزاده، مهرداد؛ هادیان، جواد؛ نجفی، فرزاد؛ محمودی، محمد و طاهری، صبا (1393، بهمن). بررسی رشد و عملکرد برخی از ارقام بابونه آلمانی (Matricaria chamomilla L.) در شمال تهران. نهمین کنگره علوم باغبانی ایران. خوزستان، اهواز، ایران.
دانائی الهام و عبدوسی، وحید (1397). پاسخ‌های فیتوشیمیایی و مورفوفیزیولوژیکی گیاه ریحان (Ocimum basilicum L.) به محلول‌پاشی برگی پلی‌آمین‌ها. فصلنامه گیاهان دارویی. ۱۸ (۶۹)، ۱۲۵-۱۳۳
زمانی، زهرا؛ نیاکان، مریم و قربانلی، مه لقا (1392). تأثیر پوتریسین برون زا بر میزان ترکیبات فنلی، فعالیت آنزیم‌های آنتی‌اکسیدان و نیترات ردوکتاز دانه‌رست گیاه بنگ‌دانه (Hyoscyomus niger) تحت تنش خشکی. نشریه پژوهش‌های اکوفیزیولوژی گیاهی ایران. 31 (3)، 78-90.
فاضل، فروغ؛ قیصری، مهدی؛ محمدیان، مرضیه و اعتمادی، نعمت‌اله (1394). تأثیر ضریب تخلیه مجاز رطوبتی بر نیاز آبیاری و شاخص‌های گیاهی چمن در آبیاری قطره‌ای زیر سطحی. مجله علوم و مهندسی آبیاری. 40 (1)، 155-165.
فرجی، عزیزه؛ عباسزاده، بهلول؛ سفیدکن، فاطمه؛ اسماعیلپور، بهروز و خاوازی، کاظم (1394). اثر سالیسیلیک اسید و پوتریسین بر رشد و ترکیب‌های اسانس مرزه (Satureja hortensis L.). مجله تحقیقات گیاهان دارویی و معطر ایران. 31 (4)، 709-722.
مظفریان، ولی‌اله (1392). شناسایی گیاهان دارویی و معطر ایران. تهران: انتشارات فرهنگ معاصر.
References
Abd El-Wahed, M. S. A., Krima, M., & Gamal, E. D. (2004). Stimulation of growth, flowering, biochemical constituents & essential oil of chamomile plant (Chamomilla recutita L.) with spermidine & stigmasterol application. BULG. Plant Physiolg, 30(1), 89-102.
Abd El-Wahed, M. S. A. (2000). Effect of stigmasterol, spermidine & sucrose on vegetative growth, carbohydrate distribution & yield of Maize Plants. Egypt. Physiology Science, 24(2), 225-239.
Adams, R. P. (2007). Identification of essential oil components by gas chromatography/mass spectrometry. Illinois: Carol Stream, Allured publishing corporation.
Akbarzadeh, M., Hadian, J., Najafi, F., Mahmudi, M., & Taheri, S. (2014, December). The study of growth and yield of some German chamomile (Matricaria chamomilla L.) cultivars in north Tehran. 9th Iranian Horticultural Science congress. Khuzestan, Ahwaz, Iran. (In Persian).
Alcazar, R., Cuevas, J., Patron, C., Altabella, M., & Tiburcio, A. F. (2006). Abscisic acid modulates polyamine metabolism under water stress in Arabidopsis thaliana. Plant Physiology, 128(2), 448-455.
Alcazar, R., Altabella, T. F., Marco, C., Bortolotti, M., Reymond, C., Koncz, P., Carrasco, A., & Tiburcio, A. F. (2010). Polyamines: molecules with regulatory functions in plant abiotic stress tolerance. Planta, 231(1), 1237-1249.
Alcázar, R., Bueno, M., & Tiburcio, A. F. (2020). Polyamines: Small amines with large effects on plant abiotic stress tolerance. Cells, 9(11), 23-73.
Ali, R. M., Abbas, H. M., & Kamal, R. K. (2007). The effects of treatment with polyamines on dry matter, oil and flavonoid contents in salinity stressed chamomile and sweet marjoram. Plant Soil and Environment, 53(3), 529-543.
An, Z. F., Jing, W., Liu, Y. L., & Zhang, W. H. (2008). Hydrogen peroxide generated by copper amine oxidase is involved in abscisic acid-induced stomatal closure in Vicia faba. Environment Experiment Botany, 59(2), 815-825.
Aydin, M., Pour, A. H., Haliloglu, K., & Tosun, M. (2015). Effect of putrescine application and drought stress on germination of wheat (Triticum aestivum L.). Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 46(1), 43-55.
British Pharmacopoeia. (2013). The British  Pharmacopoeia Commission. London: Renouf Publishing Company Limited.
Capell, T., Escobar, C., Lui, H., Burtin, D., Lepri, O., & Christou, P. (1998). Over-expression of the oat arginine decarboxylase cDNA in transgenic rice (Oryza sativa L.) affects normal evelopment patterns in vitro and results in putrescine accumulation in transgenic plants. Theoretical Applied Genetic, 97, 246-254.
Cseke, L. J., & Kaufan, P. B. (1999). Regulation of metabolite synthesis in plants. In Natural Products from Planta. edited by Kaufam, P. B. Cseke, L. J., Warber, S., Duke, J. A., & Brielmann, H. L. Florida: CRC Press.
Danaee, E., & Abdossi, V. (2019). phytochemical and morphophysiological responses in Basil (Ocimum basilicum L.) plant to application of polyamines. Journal of Medicinal Plants, 18(69), 125-133. http://dorl.net/dor/20.1001.1.2717204.2019.18.69.15.6. (In Persian).
Faraji, A., Esmailpoor, B., Sefidkon, F., Abaszadeh, B., & Khavazy, K. (2015). Effect of salicylic acid and putrescine on growth and essential oil compounds of summer savory (Satureja hortensis L.). Iranian Journal of Medicinal and Aromatic Plants, 31(3), 709-722. (In Persian).
Fazel, F., Gheysari, M., Mohammadian, M., & Etemadi, N. (2016). Effect of Maximum Allowable Depletion on Irrigation Use and Plant Parameters of Grass under Subsurface Drip Irrigation Management. Journal of Irrigation Sciences and Engineering (JISE), 40, 155-165. (In Persian).
González-Hernández, A. I., Scalschi, L., Vicedo, B., Marcos-Barbero, E. L., Morcuende, R., & Camañes, G. (2022). Putrescine: A Key Metabolite Involved in Plant Development, Tolerance and Resistance Responses to Stress. International journal of molecular sciences, 23(6), 2971.
Groppa, M. D., & Benavides, M. P. (2008). Polyamines and abiotic stress: recent advances. Amino Acids, 34, 35-45.
He, L., Ban, Y., ınoue, H., Matsuda, N., Liu, J., & Moriguchi, T. (2008). Enhancement of spermidine content and antioxidant capacity in transgenic pear shoots overexpressing apple spermidine synthase in response to salinity and hyperosmosis. Photochemistry, 69, 2133-2141.
Kumar, S., Das, M., Singh, A., Ram, G., Mallavarapu, G. R., & Ramesh, S. (2001). Composition of the essential oils of the flowers, shoots and roots of two cultivars of Chamomilla recutita. Medical Aromatic Plant Science, 23(4), 617-623.
Liu, J. H., Kitashiba, H., Wang, J., Ban, Y., & Moriguchi, T. (2007). Polyamines and their ability to provide environmental stress tolerance to plants. Plant Biotechnology, 24, 117-126.
Liu, H.P., Jiang, M.Y., Zhou, Y. F., & Liu, Y. L. (2005). Production of polyamines is enhanced by endogenous abscisic acid in maize seedlings subjected to salt stress. Plant Biology, 47, 1326-1334.
Mahboub, M. H., Abd El Aziz, N. G., & Mazhar, A. M. A. (2011). Response of Matricaria recutita plant to foliar spray with putrescine and thiamine on growth, flowering and photosynthetic pigments. American-Eurasian Journal of Agriculture and Environmental Science, 10(5), 769-775.
Mann, C., & Staba, E. J. (1986). The chemistry, pharmacology, and commercial formulations of Chamomile. In Herbs, Spices, and Medicinal Plants: Recent Advances in Botany, Horticulture, and Pharmacology. edited by Craker, L. E., Simon, J. E. Phoenix: Oryx Press.
Marco, F., Alcázar, R., Tiburcio, A. F., & Carrasco, P. (2011). Interactions between polyamines and abiotic stress pathway responses unraveled by transcriptome analysis of polyamine overproducers. Omics: a journal of integrative biology, 15(11), 775-781.
Mohammadi-Cheraghabadi, M., Modarres-Sanavy, S. A. M., Sefidkon, F., Rashidi-Monfared, S., & Mokhtassi-Bidgoli, A. (2021). Improving water deficit tolerance of Salvia officinalis L. using putrescine. Scientific Reports, 11(1), 1-15.
Mozaffarian, V. (2013) Identification of Medicinal and Aromatic Plants of Iran. Tehran: Farhang-e-Moaser publication. (In Persian).
Nieves, N., Martinez, M. E., Castillo, R., Blanco, M. A., & Gonzalez-Olmedo, J. L. (2001). Effect of abscisic acid and jasmonic acid on partial dessication of encapsulated somatic embryos of sugarcane. Plant Cell Tissue Organ Cult, 65, 15-21.
Abd Elbar, O. H., Farag, R. E., & Shehata, S. A. (2019). Effect of putrescine application on some growth, biochemical and anatomical characteristics of Thymus vulgaris L. under drought stress. Annals of Agricultural Sciences, 64, 129-137.
Ortega-Amaro, M. A., Rodriguez-Kessler, M., Becerra-Flora, A., & Jimenez-Bremont, J. F. (2012). Modulation of Arabidopsis CYCB1 expression patterns by polyamines and salt stress. Acta Physiology, 34, 461-469.
Sangwan, N. S., Farooqi, A. H. A., Sabih, F., & Sangwan, R. S. (2001). Regulation of essential oil production in plants. Plant Growth Regulators, 34, 3-21.
Schilcher, H., Imming, P., & Goeters, S. (2005). Active Chemical Constituents of Matricaria chamomilla L. syn. Chamomilla recutita (L.) Rauschert. In Chamomile: Industrial Profiles. edited by Franke, R., Schilcher, H. Florida: CRC Press.
Sharafzadeh, Sh., Bazrafshan, F., & Bayatpoor, N. (2012). Effect of naphthaleneacetic acid and spermidine on essential oil constituents of German chamomile. Inter. Agriculture Crop Science, 4(23), 1803-1806.
Sharif moghaddasi, M. (2011). Study on chamomile (Matricaria chamomilla L.) usage and farming. Environment Biology, 5, 1446-1453.
Verma, S., & Mishra, S. N. (2005). Putrescine alleviation of growth in salt stressed Brassica juncea by inducing antioxidative defense System. Plant Physiology, 162, 669-677.
Yamaguchi, K., Takahashi, Y., Berberich, T., Imai, A., Takahashi, T., Michael, A. J., & Kusano. T. (2007). A protective role for the polyamine spermine against drought stress in Arabidopsis. Biochemical and Biophysical Research Communications, 352, 486-490.
Yamasaki, H., & Cohen, M. F. (2006). NO signal at the crossroads: polyamine-induced nitric oxide synthesis in plants? Trends Plant Science, 11, 522-524.
Yiu, J., Juang, L., Fang, D., Liu, Ch., & Wu, Sh. (2009). Exogenous putrescine reduces flooding- induced oxidative damage by increasing the antioxidant properties of Welsh onion. Science Horticulture, 120(3), 306-314.
Youssef, A. A., Aly, M. S., Abou Zied, E. N., Iliey, L., & Titiana, S. (2002). Effect of some growth substances on mass production and volatile oil yield of Mentha piperita E.”Bulgaro”. Egyptian Journal of Applied Science, 17(11), 610-623.
Zamani, Z., Niakan, M., & Ghorbani, M. (2013). Effect of endogenous putrescine on phenolic compounds content, anthioxidant enzymes and nitrate reductase activity of hyoscyomus (Hyoscyomus niger) seedlings under drought stress. Journal of Iranian Plant Ecophysiological Research, 8(31), 78-90. (In Persian).
Zhang, W., Jiang, B., Li, W., Song, H., Yu, Y., & Chen, J. (2009). Polyamines enhance chilling tolerance of cucumber (Cucumis sativus L.) through modulating antioxidative system. Science Horticulture, 122, 200-208.