Document Type : Research Paper


1 Ph.D. Candidate, Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

3 Associate Professor, Department of Cultivation and Development of Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran

4 Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

5 Associate Professor, Department of Horticultural Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran


The present study has been conducted to investigate the duration of growth period of different cannabis (Cannabis sativa L.) ecotypes and their responses to water stress based on a factorial completely randomized experiment design in the greenhouse condition at the University of Tehran in 2017. Irrigation levels include 50%, 75%, and 100% of field capacity, with the ecotypes being Urmia, Sanandaj, Tabriz, Dasht-e-Moghan, Rasht, Khomein, Daran, Qom, Shahroud, Kerman, Tabas, and Saravan. Results show that Rasht and Khomein ecotypes have had the highest and lowest duration of germination phase and growth degree-days (GDD1), respectively. The highest duration of vegetative, flowering and maturation phases belong to Dasht-e-Moghan, Rasht, and Dasht-e-Moghan ecotypes with 71.33, 30, and 46.66 (days), respectively, at 100% field capacity. The lowest durations of these phases could be seen in Tabas, Tabriz, and Saravan ecotypes with 42, 16, and 17 (days), respectively, at 50% field capacity. Also, the highest values of growth degree-days for vegetative (GDD2), flowering (GDD3), and maturation (GDD4) phases  for Dasht-e-Moghan, Kerman, and Dasht-e-Moghan ecotypes with 1788, 836, and 1169 (0C.d), respectively, at 100% field capacity, with their lowest values belonging to Tabas, Tabri,z and Saravan with 1039, 413, and 448 (0C.d), respectively, at 50% field capacity. Based on growth period duration and cumulative growth degree-days (total GDD), Tabas and Dasht-e-Moghan ecotypes are found as earliest and latest ecotypes, respectively.


Ahmed, S. A., Ross, S. A., Slade, D., Radwan, M. M., Zulfiqar, F., & ElSohly, M. A. (2008). Cannabinoid ester constituents from high-potency Cannabis sativa. Journal of natural products, 71(4), 536-542.‏
Aladić, K., Jarni, K., Barbir, T., Vidović, S., Vladić, J., Bilić, M., & Jokić, S. (2015). Supercritical CO2 extraction of hemp (Cannabis sativa L.) seed oil. Industrial Crops and Products, 76, 472-478.‏
Amaducci, S., Zatta, A., Pelatti, F., & Venturi, G. (2008). Influence of agronomic factors on yield and quality of hemp (Cannabis sativa L.) fibre and implication for an innovative production system. Field Crops Research, 107(2), 161-169.‏
Anderson, R. D. (2018). Effects of Nitrogen Fertilizer Rate, Timing, and Herbicide Use on Industrial Hemp (Cannabis Sativa L.).‏ Masters Theses & Specialist Project.
Anwar, F., Latif, S., & Ashraf, M. (2006). Analytical characterization of hemp (Cannabis sativa) seed oil from different agro‐ecological zones of Pakistan. Journal of the American Oil Chemists' Society, 83(4), 323-329.‏
Ardakani, M, R., Abbaszadeh, B., Sharifi, E., Lebaschi, M, H., Moaveni, P., & Mohebati, F. (2010). Drought stress on growth indices of lemongrass (Melissa officinalis L.). Journal Plants and Ecosystems, 21, 47-58.
Asghari-Pour, M. R., & Rashed-Mohassel, M. H. (2007). The effect of plant population and nitrogen fertilizer on fiber yield of hemp (Cannabis sativa L.). Iranian Journal of Field Crops Research, 4(2), 1-14.
Bahador, M., & Tadayon, M. (2018). Effect of deficit irrigation and zeolite levels on phenology, oil yield and water use efficiency of hempHemp.‏ Iranian Journal of Field Crop Science, 49(3), 25-38.
Bahreininezhad, B., & Razmju, J. (2014). Effects of Drought Stress on Physiological Growth Indices and Phenological Characteristics of Thyme. Journal of Plant Process and Function,7(3),67-79.
Baldini, M., Ferfuia, C., Zuliani, F., & Danuso, F. (2020). Suitability assessment of different hemp (Cannabis sativa L.) varieties to the cultivation environment. Industrial Crops and Products, 143, 111860.‏
Bazzazi, N., Khodambashi, M., & Mohammadi, S. (2013). The effect of drought stress on morphological characteristics and yield components of medicinal plant fenugreek. Journal of Crop Production and Processing, 3(8), 11-23.
Behtari, B., Ghassemi-Golezani, K., Nasab, A. D. M., Salmasi, S. Z., & Toorchi, M. (2010). Effect of Water Deficit on Morphological Traits and Water Use Efficiency of Two Soybean Cultivars. Journal of Agricultural science and Sustainable Production, 2(4), 11-21.
Cannoy, D. C. (2015). Green Gold-a Cannabis Sativa L. Lucis Suitability Analysis for West Virginia.‏ Marshall University, 14(2), 45-47.
Chaves, M. M., Maroco, J. P., & Pereira, J. S. (2003). Understanding plant responses to drought—from genes to the whole plant. Functional plant biology, 30(3), 239-264.‏
Dolferus, R., Ji, X., & Richards, R. A. (2011). Abiotic stress and control of grain number in cereals. Plant science, 181(4), 331-341.‏
Ghanbari, A. A. (2015). Developmental stages and phenology of common bean genotypes under normal irrigation and water deficit conditions.‏ Agronomy Journal, 107, 190-199.
Hosseini, S.M. (2009). Study of growth degree day Index of Orange, Cotton, Wheat and Rice in gharakhil. Journal of Mazandaran Province Meteorological Office-Spring and summer seasons.
Irannezhad, H., Poshtkohi, M., Piri, P., & Javanmardi, Z. (2007). The cultivation of medicinal plants and oily plants (Cannabis, Flex oil & castor bean oil). Ayezh Press. Tehran. Iran. pp: 128.
Jentsch, A., Kreyling, J., Boettcher‐Treschkow, J. E. G. O. R., & Beierkuhnlein, C. (2009). Beyond gradual warming: extreme weather events alter flower phenology of European grassland and heath species. Global Change Biology, 15(4), 837-849.‏
Khalili, M., Naghavi, M. R., & Pour-Aboughadareh, A. (2015). Evaluation of Grain Yield and Some of Agro-Morphological Characters in Spring Safflowers Genotypes under Irrigated and Rainfed Conditions. Journal of Crop Breeding, 7(16), 139-148.
Khanduri, V. P., Sharma, C. M., & Singh, S. P. (2008). The effects of climate change on plant phenology. The Environmentalist, 28(2), 143-147.‏
Kumar, A., Omae, H., Egawa, Y., Kashiwaba, K., & Shono, M. (2006). Adaptation to heat and drought stresses in snap bean (Phaseolus vulgaris L.) during the reproductive stage of development. Japan Agricultural Research Quarterly: JARQ, 40(3), 213-216.‏
Mirhaji, T., Sanadgol, A. A., Ghasemi, M. H., & Nouri, S. (2010). Application of Growth Degree-Days in determining phenological stages of four grass species in Homand Absard Research Station. Iranian Journal of Range and Desert Research, 17(3), 362-376.
Mulligan, K., Laniel, L., Potter, D., Hughes, B., Vandam, L., & Skarupova, K. (2012). Cannabis production and markets in Europe. C. Carpentier (Ed.). Publ. Office of the Europ. Union‏ p. 243.
Parsa, M., Ganjali, A., Rezaeanzadeh, A., & Nezami, A. (2011). Effect of supplemental irrigation on yield and growth indices of chickpea (Cicer arietinum L.). Iranian Journal of Field Crops Research, 9(3), 310-321.
Riahi, L., Shokrpour, M., Salami, S. A., & Khandan, A. (2016). Some quantitative traits associations and their application in characterization of Iranian accessions of Cannabis (Cannabis sativa L.). Iranian Journal of Filed Crop Science, 46(4), 629-639.
Saadati, A., Pourtahmasebi, K., Salami, S.A., & Oladi, R. (2013). Xylem and Bast Fiber Properties of Six Iranian Hemp Population. Iranian Journal of Natural Resources, 68(1), 121-32.
Sanadgol, A. (2003). The short-term effects of two grazing systems and three grazing intensities on the phenology of Bromus tomentellus Boiss. Iranian journal of Range and Desert Research, 17(3), 321-337.
Taher Abadi, Sh., Goldani, M., Taher Abadi, Sh., & Fazeli Kakhki, F. (2015). Determination of Cardinal Temperatures in the Seeds of Henbane, Aconite and Hemp.jornal of plant protection, 29(1), 16-22.
Tommasini, L., Svensson, J. T., Rodriguez, E. M., Wahid, A., Malatrasi, M., Kato, K., & Close, T. J. (2008). Dehydrin gene expression provides an indicator of low temperature and drought stress: transcriptome-based analysis of barley (Hordeum vulgare L.). Functional & integrative genomics, 8(4), 387-405.‏
Van der Werf, H. M. G., Brouwer, K., Wijlhuizen, M., & Withagen, J. C. M. (1995). The effect of temperature on leaf appearance and canopy establishment in fiber hemp (Cannabis sativa L.). Annals of Applied Biology, 126(3), 551-561.
Van Dijk, H., & Hautekeete, N. (2007). Long day plants and the response to global warming: rapid evolutionary change in day length sensitivity is possible in wild beet. Journal of Evolutionary Biology, 20(1), 349-357.‏
Yasari, T., Shahsavari, M. R., Barzegar, A., & Omidi, A. H. (2005). Study of developmental stages and relationship between of them and seed yield in ten advanced safflower genotype.‏ Pajouhesh & Sazandegi, 68, 75-83.