The aim of this study was to evaluate the yield of cucumber genotypes in two seasons, and to identify cucumber genotypes with high yield and stability. This research was carried out in the greenhouse of the Department of Horticultural Sciences, University of Guilan as a factorial experiment in a completely randomized design in autumn and spring seasons with 9 cucumber genotypes in three replications. The results showed that in terms of number of female flowers in autumn and spring, genotype C1 with an average of 16.7 per plant and genotype C8 with an average of 7.0 per plant are the best, respectively. Also in terms of leaf area, C10 genotype with an average of 2866.6 cm2 in autumn and C8 genotype with an average of 9340.990 cm2 in spring are the best and can be used to achieve suitable hybrids for each growing season. Also, the results of principal components analysis and population biplot showed that C8 genotype is a suitable genotype for cultivation in two cropping seasons in terms of stability of yield-related traits and vegetative traits. Finally, in terms of number of fruits, genotype C1 with an average of 5.0 per plant in autumn and C10 genotype with an average of 5.0 per plant in spring were superior.