Document Type : Research Paper


1 Corresponding Author, Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran. E-mail:

2 Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran. E-mail:

3 Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran. E-mail:

4 Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran. E-mail:


In order to study the effects of water limitation, biofertilizers and nanosilicon on compatible osmolytes and biochemical traits of triticale, an experiment as factorial was conducted based on randomized complete block design with three replications in research greenhouse faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabil during 2020. The experimental factors were included irrigation at three levels (full irrigation as control; irrigation withholding at 50% of heading as moderate water limitation and irrigation withholding at 50% of booting stages as severe water imitation), application of bio fertilizers (no application of bio fertilizers as control, application of vermicompost, Mycorrhiza, both application vermicompost and Mycorrhiza), foliar application of nano silicon (foliar application with water as control and foliar application of 2 g.L-1 nano silicon). The results showed that both application of vermicompost, Mycorrhiza and foliar application nano silicon under irrigation withholding in booting stage, increased the activity of catalase, polyphenol oxidase enzymes, proline and soluble sugar content (49.17, 50.64, 44.92 and 52.22% respectively) compared to no application of bio fertilizers and nano silicon under normal irrigation condition. Also, both application of vermicompost, Mycorrhiza and foliar application of nano silicon in normal irrigation condition decreased hydrogen peroxide and malondialdehyde content (55.34 and 53.64% respectively) and grain yield increased 59.52% compared to no application of bio fertilizers and nano silicon under irrigation withholding in booting stage condition. It seems that the application of biofertilizers and nano silicon can increase grain yield triticale under water limitation due to improving the biochemical traits.


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