Numerous studies have been performed on the effect of rootstock and cultivar on physicochemical traits of apple fruit and the results have shown different effects of these two factors. In order to study three vegetative rootstocks on some quantitative and qualitative characteristics of apple cultivars "Golden Delicious" and "Red Delicious", an experiment in factorial design based on randomized complete blocks with four replications in orchard of Mashhad Agricultural Research Center was performed in 2021. Fruit quantitative and qualitative characteristics: weight, volume, density, length, diameter, firmness, pH, total soluble solids, phenol content, antioxidant properties and anthocyanin was measured. The highest amount of total phenol (652.9 μg.g-1FW) and anthocyanin (35.9 mg.g-1FW) was measured in "Red delicious" on M9 rootstock. The results of this study showed that "Red delicious" had the highest amount of diameter (78/64 mm) and weight (166/9 g). The highest amount of fruit firmness in M9 rootstock (5/64 kgcm-3) was observed. In general, the results of this study indicated a different effect of rootstock and cultivar interaction on the studied characteristics. Depending on the purpose of gardener, different grafting compounds can be recommended. "Red delicious" on M9 and MM106 rootstock with highest amount of total phenol and anthocyanin was suggested as a valuable grafting compounds in terms of health benefits under Mashhad Climatic Conditions.