Response of Seed Germination and Seedling Growth of Licorice to Chemical Scarification and Gibberellic Acid Levels

Ghanbari, Jalal; Besharati-Far, Marzieh; Khajoei-Nejad, Gholamreza

doi:10.22059/jci.2021.328615.2595

Document Type : Research Paper

Authors

¹ Corresponding Author, Department of Plant Production and Genetics, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran. E-mail: jalalghanbari@agr.uk.ac.ir

² Department of Plant Production and Genetics, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran. E-mail: marziehbesharati@gmail.com

³ Department of Plant Production and Genetics, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran. E-mail: khajoei@uk.ac.ir

https://doi.org/10.22059/jci.2021.328615.2595

Abstract

Natural habitats of licorice have decreased dramatically while the demand for this plant rises. Licorice seeds exhibit a low germination rate due to secondary dormancy imposed by the hard seed coat. In this study, conducted in the spring of 2021 at Shahid Bahonar University of Kerman, Iran, the interaction effect of sulfuric acid (95-97%, 60 min) scarification with gibberellic acid (GA) concentrations (0, 100, 250, 500, and 1000 mgL^-1) has been studied in a factorial experiment based on a completely randomized design with four replications. Results show that although there has been no difference in germination between GA levels for scarified seeds, GA at 250 mg L^-1 improves germination by 36%, compared to the control in non-chemically scarified ones. In contrast, increasing in GA concentrations increased seedling length, plant height, number of leaves and compound leaves, leaf area, fresh and dry weight of shoot and root, while decreased chlorophyll index (SPAD). As a result, chemical scarification, application of GA at 1000 mg L^-1, and germination at room temperature can effectively increase the germination rate and uniformity as well as early growth of licorice plant.

Keywords

20.1001.1.20088337.1401.24.4.19.1

References

Abudureheman, B., Liu, H., Zhang, D., & Guan, K. (2014). Identification of physical dormancy and dormancy release patterns in several species (Fabaceae) of the cold desert, north-west China. Seed Science Research, 24(2), 133. https://doi.org/10.1017/S0960258514000063

Ahmad, F., Kamal, A., Singh, A., Ashfaque, F., Alamri, S., Siddiqui, M. H., & Khan, M. I. R. (2021). Seed priming with gibberellic acid induces high salinity tolerance in Pisum sativum through antioxidants, secondary metabolites and up‐regulation of antiporter genes. Plant Biology, 23, 113-121. https://doi.org/10.1111/plb.13187

Amooaghaie, R. (2009). The effect mechanism of moist-chilling and GA3 on seed germination and subsequent seedling growth of Ferula ovina Boiss. The Open Plant Science Journal, 3(1), 22-28.

Baskin, J. M., & Baskin, C. C. (2004). A classification system for seed dormancy. Seed Science Research, 14(1), 1-16. https://doi.org/10.1079/SSR2003150

Bhatt, A., Carón, M. M., Verheyen, K., Elsarrag, E., & Alhorr, Y. (2016). Germination and seedling performance of five native legumes of the Arabian Desert. Flora-Morphology, Distribution, Functional Ecology of Plants, 220, 125-133. https://doi.org/10.1016/j.flora.2016.03.002

Carruggio, F., Onofri, A., Impelluso, C., Giusso del Galdo, G., Scopece, G., & Cristaudo, A. (2020). Seed dormancy breaking and germination in Bituminaria basaltica and B. bituminosa (Fabaceae). Plants, 9(9), 1110. https://doi.org/10.3390/plants9091110

Cornea-Cipcigan, M., Pamfil, D., Sisea, C. R., & Mărgăoan, R. (2020). Gibberellic acid can improve seed germination and ornamental quality of selected cyclamen species grown under short and long days. Agronomy, 10(4), 516. https://doi.org/10.3390/agronomy10040516

Ghadiri, H., & Bagherani, T. N. (2000). Effects of scarification and temperature on germination of licorice (Glycyrrhiza glabra L.) seeds, 2(4), 257-262. Retrieved from http://jast.modares.ac.ir/article-23-11099-en.html

Graeber, K. A. I., Nakabayashi, K., Miatton, E., Leubner‐Metzger, G., & Soppe, W. J. J. (2012). Molecular mechanisms of seed dormancy. Plant, Cell & Environment, 35(10), 1769-1786. https://doi.org/10.1111/j.1365-3040.2012.02542.x

Hayashi, H., & Sudo, H. (2009). Economic importance of licorice. Plant Biotechnology, 26(1), 101-104. https://doi.org/10.5511/plantbiotechnology.26.101

Jiang, M., Zhao, S., Yang, S., Lin, X., He, X., Wei, X., … Zhang, J. (2020). An “essential herbal medicine”-Licorice: A review of phytochemicals and its effects in combination preparations. Journal of Ethnopharmacology, 249, 112439. https://doi.org/10.1016/j.jep.2019.112439

Jin, Q., Duan, L., Li, J., Dong, X., Tian, X., Wang, B., & Li, Z. (2006). Scarification damages by sulphuric acid and their effects on vigour, germination and emergence of Glycyrrhiza uralensis Fisch seeds. Seed Science and Technology, 34(1), 227-231. https://doi.org/10.15258/sst.2006.34.1.27

Kucera, B., Cohn, M. A., & Leubner-Metzger, G. (2005). Plant hormone interactions during seed dormancy release and germination. Seed Science Research, 15(4), 281-307. https://doi.org/10.1079/SSR2005218

Mao, P.-S., Wang, Y.-H., Wang, X.-G., Lian, J.-J., & Huang, Y. (2008). Conditions and Stimulation for Germination in Glycyrrhiza uralensis Fisch Seeds. Agricultural Sciences in China, 7(12), 1438-1444. https://doi.org/10.1016/S1671-2927(08)60400-9

Nadjafi, F., Bannayan, M., Tabrizi, L., & Rastgoo, M. (2006). Seed germination and dormancy breaking techniques for Ferula gummosa and Teucrium polium. Journal of Arid Environments, 64(3), 542-547. https://doi.org/10.1016/j.jaridenv.2005.06.009

Rafieiolhossaini, M., Tadayon, M. R., & Mazhari, M. (2014). The effect of dormancy breaking treatments on seed germination of Licorice medicinal plant (Glycyrrhiza glabra L.). Journal of Crops Improvement, 16(4), 809-817.

Solichatun, S., Santosa, S., Dewi, K., & Pratiwi, R. (2016). The effects of physical and hormonal treatments on dormancy breaking and the changes in seed coat ultrastructure of Delonix regia. Nusantara Bioscience, 8(1), https://doi.org/10.13057/nusbiosci/n080117

Verma, S., Sharma, R. K., & Shrivastava, D. K. (2000). Seed germination, viability and invigoration studies in medicinal plants of commercial value. Journal of Medicinal and Aromatic Plant Sciences, 22(4a), 426-428.

Vishal, B., & Kumar, P. P. (2018). Regulation of Seed Germination and Abiotic Stresses by Gibberellins and Abscisic Acid. Frontiers in Plant Science, 9, 838. https://doi.org/10.3389/fpls.2018.00838

Wheeler, A. W., & Humphries, E. C. (1963). Effect of gibberellic acid on growth, gibberellin content, and chlorophyll content of leaves of potato (Solanum tuberosum). Journal of Experimental Botany, 14(1), 132-136. https://doi.org/10.1093/jxb/14.1.132

Yang, L.-E., Peng, D.-L., Li, Z.-M., Huang, L., Yang, J., & Sun, H. (2020). Cold stratification, temperature, light, GA3, and KNO3 effects on seed germination of Primula beesiana from Yunnan, China. Plant Diversity, 42(3), 168-173. https://doi.org/10.1016/j.pld.2020.01.003

Response of Seed Germination and Seedling Growth of Licorice to Chemical Scarification and Gibberellic Acid Levels

References

References

Volume 24, Issue 4December 2022Pages 1311-1324

Volume 24, Issue 4
December 2022
Pages 1311-1324