Document Type : Research Paper

Author

Assistant Professor, Department of Agriculture, Payame Noor University, Tehran, Iran.

Abstract

In order to investigate the effects of foliar application of amino acids fertilization and mycorrhizal fungi symbiosis on quantitative and qualitative characteristics of Calendula officinalis L., a factorial layout has been conducted, based on a randomized complete block design with three replications at the field of the research farm of Islamic Azad University, Takestan Branch during 2017-2018 and 2018-2019 years. Experimental factors include three species of mycorrhiza fungi (control, Glomus mosseae, G.etunicatum and G.intradices) and Soren amino acid fertilizer levels (this commercial compound contains a variety of essential amino acids for the plant) include no foliar application and foliar application in two stages of stem and flowering. The results show that inoculation with mycorrhiza species has been significant for all studied traits in this test. Also, the effect of amino acid fertilizer has had a significant effect on all traits, except essential oil percentage and extract yield. Among the mycorrhizae used in this experiment, Glomus mosseae is able to be superior over other fungi in most traits. The highest seed yield is obtained from amino acid fertilizer and Glomus mosseae with 2343 kg / ha and the highest essential oil yield is in Glomus mosseae with 7.74 kg / ha. Based on the results, it can be concluded that the use of amino acid fertilizers with inoculation with mycorrhiza has a positive effect on the traits of marigold.

Keywords

Abdel Aziz, N. G., Mazher, A. A. M., & Farahat, M. M., (2010). Response of vegetative growth and chemical constituents of Thuja orientalis L. plant to foliar application of different amino acids at Nubaria. Journal of American Science, 6(3), 295-301.
Abdel-Mawgoud, A. M. R., El-Bassiouny, A. M., Ghoname, A., & Abou-Hussein, S. D. (2011). Foliar Application of Amino Acids and Micronutrients Enhance Performance ofGreen Bean Crop under Newly Reclaimed Land Conditions. Australian Journal of Basic and Applied Sciences, 5(6), 51-55.
Aghababaei, F., Raiesi, F., & Nadian, H. (2011). Effect of mycorrhizal symbiosis on nutrient uptake by some commercial almond genotypes in a sandy loam soil. Journal of Soil Research, 25(2), 138-147.
Akbari, I., & Gholami, A. (2015). Evaluation of mycorrhizal fungi, vermicompost and humic acid on essence yield and root colonization of fennel. Iranian Journal of Field Crops Research, 13(4), 840-853. (In Persian).            
Alshaal, T., & El-Ramady, H. (2017). Foliar Application: from Plant Nutrition to Biofortification. The Environment, Biodiversity & Soil Security, 1, 71-83. DOI: 10.21608/jenvbs.2017.1089.1006.
Amanifar, S., & Toghranegarb, Z. (2020). The efficiency of arbuscular mycorrhiza for improving tolerance of Valeriana officinalis L. and enhancing valerenic acid accumulation under salinity stress. Industrial Crops & Products, 147 (2020) 112234. DOI:10.1016/j.indcrop.2020.112234.
Ameri, A. A., Nassiri Mahallati, M., & Rezvani Moghadam, P. (2007). Effects of different nitrogen levels and plant density on flower, essential oils and extract production and nitrogen use efficiency of Marigold (Calendula officinalis). Iranian Journal of Crop Research, 5(2), 315-325. (In Persian).
Aminifard, M. H., Gholami, M., Bayat, H., & Moradi Nezhad, F. (2020). The Effect of Fulvic Acid and Amino Acid Application on Physiological Characteristics, Growth and Yield of Coriander (Coriandrum sativum L.) as a Medicinal Plant. Journal of Agroecology, 12(3), 373-388. (In Persian).
Amouzegar, M., Abbaspour, A., Shahsavani, S., Asghari, H. R., & Parsaeiyan, M. (2016). Effects of phosphorus fertilizers and Arbuscular Mycorrhiza fungi symbiosis with sunflower on Pb availability in a contaminated soil. Journal of Water and Soil Science, 19(74),39-50.(In Persian).
Asadi, M., Nasiri, Y., Mola Ali Abasiyan, S., & Morshedloo, M. R., (2018). Evaluation of Quantitative and Qualitative Yiled of Peppermint under Amino Acids, Chemical and Organic Fertilizers. Journal of Agricultural Knowledge and Sustainable Production, 28(2), 257-275. (In Persian).
Calvo, P., Nelson, L., & Kloepper, J. (2014). Agricultural uses of plant biostimulants. Plant Soil, 383, 3-41.
Chitrakar, B., Zhang, M., & Bhandari, B. (2019). Edible flowers with the common name “marigold”: Their therapeutic values and processing. Trends in Food Science & Technology, 89, 76-87. DOI: 10.1016/j.tifs.2019.05.008.
Diagne, N., Ngom, M., Ibrahima Djighaly, P., Fall, D., Hocher, V., & Svistoonof, S. (2020). Roles of Arbuscular Mycorrhizal Fungi on Plant Growth and Performance: Importance in Biotic and Abiotic Stressed Regulation. Diversity. 12, 370. DOI:10.3390/d12100370.
El-Abagy, H.M., El-Tohamy, W.A., Abdel-Mawgoud, A.M.R., & Abou-hussein, S.D. (2014). Effect of Different Amino Acid Sources and Application Rates on Yield and Quality of Onion in the Newly Reclaimed Lands. Middle East Journal of Agriculture Research, 3(1), 81-88.
El-Said, M.A.A., & Mahdy, A.Y., (2016). Response of two wheat cultivars to foliar application with amino acids under low levels of nitrogen fertilization. Middle East Journal of Agriculture Research, 5, 462-472.
Ertani, A., Pizzeghelio, D., Altissimo, A., & Nardi, S. (2013). Use of meat hydrolyzate derived from tanning residues as plant biostimulant for hydroponically grown maize. Journal of Plant Nutrition and Soil Science, 176, 287-296. DOI: 10.1002/jpln.201200020.
Escher, G.B., Borges, L.D.C., Santos, J.S., Cruz, T.M., Marques, M.B., do Carmo, M.A.V., Azevedo, L., Furtado, M.M., Sant, Ana, A.S., Wen, M.C., et al. (2019). From the field to the pot: Phytochemical and functional analyses of Calendula officinalis L. flower for incorporation in an organic yogurt. Antioxidants, 8, 559. DOI: 10.3390/antiox8110559.
Evelin, H., Devi, T.S., Gupta, S., & Kapoor, R., (2019). Mitigation of salinity stress in plants by arbuscular mycorrhizal symbiosis: current understanding and new challenges. Frontiers in Plant Science, 10, 470. DOI: 10.3389/fpls.2019.00470.
Ghaith, R.H., & Galal, R.M. (2014). Response of Pea Plant (Pisum sativum L.) Growth and Yield for Spraying of Amino Acid and Boron. Egyptian Journal of Applied Science, 29, 154-173.
Ghazi Manas, M., Banj Shafiee, S., Haj Seyed Hadi, M.R., & Darzi, M.T., (2013). Effects of vermicompost and nitrogen on quantitative and qualitative yeild of chamomile (Matricaria chamomilla L.). Journal of Applied Research on Medicinal and Aromatic Plants, 29(2), 269-280. (In Persian with English Summary)
Golubkina, N., Logvinenko, L., Novitsky, M., Zamana, S., Sokolov, S., Molchanova, A., Shevchuk, O., Sekara, A., Tallarita, A., & Caruso, G., (2020). Yield, Essential Oil and Quality Performances of Artemisia dracunculus, Hyssopus officinalis and Lavandula angustifolia as Affected by Arbuscular Mycorrhizal Fungi under Organic Management. Plants, 9, 375. doi: 10.3390/plants9030375.
Golzadeh, H., Mehrafarin, A., Naghdi Badi, H., Fazeli, F., Ghaderi, A., & Zarincheh, N., (2011). Effects of bio-stimulants on quantitative and qualitative yield of German chamomile. Journal of Medicinal Plants, 11(41), 195-207. (In Persian).
Guang-Ming, H., Ying-Ning, Z., Qiang-Sheng, W., Yong-Jie, X., & Kamil K. (2020). Mycorrhizal roles in plant growth, gas exchange, root morphology, and nutrient uptake of walnuts. Plant, Soil and Environment, 66(6), 295-302. DOI.org/10.17221/240/2020-PSE.
Haghir Ebrahimabadi, A., Hatami, M., Karimzadeh Asl, K., & Ghorbanpour, M. (2017). Effect of Mycorrhizal Fungi and Biophosphor Fertilizer on Growth Features, Yield and Yield Components, and Essntial Oil Constituents in Cuminum cyminum L. Journal of Medicinal Plants, 17(2), 174-184. (In Persian).
Haj Seyed Hadi, M. R., & Rezaee Ghale, H. (2016). Effects of vermicompost and foliar application of amino acids and urea on quantitative and qualitative yield of chamomile (Matricaria chamomilla L.). Iranian Journal of Medicinal and Aromatic Plants, 31(6), 1058-1070. (In Persian).
Halpern, M., Bar-Tal, A., Ofek, M., Minz, D., Muller, T., & Yermiyahu, U. (2015). The Use of Biostimulants for Enhancing Nutrient Uptake. In: Sparks, D.L. (Ed.), Advances in Agronomy, 141-174. DOI: 10.1016/bs.agron.2014.10.001.
Hamzehii, J., & Sadeghi Mayabadi, F. (2013). Effect of Irrigation Cycle and Arbuscular Mycorrhiza on Chlorophyll Index, Yield and Yield Components of Grain Sorghum. Journal of Production and Processing of Crops and Horticulture. 4(12), 211-220. (In Persian).
Hounsome, N., Hounsome, B., Tomos, D., & Edwards-Jones, G. (2008). Plant metabolites and nutritional quality of vegetables. Journal of Food Science, 73(4), 48-65. http://dx.doi.org/10.1111/j.1750-3841.2008.00716.x
Jami, N., Rahimi, A., Sedaghati, E., & Naghizadeh, M. (2019). Effects of Mycorrhiza Inoculation and Vermicompost Levels on some Nutrient Concentrations and Corm Criteria of Saffron (Crocus sativus L.) under Kerman Climatic Conditions. Journal of Saffron Research (semi-annual), 7(2), 217-234. http://dx.doi.org/10.22077/jsr.2019.1990.1074. (In Persian).
Khaosaad, T., Vierheilig, H., Nell, M., ZitterlEglseer, K., & Novak, J. (2006). Arbuscular mycorrhiza alters the concentration of essential oils in oregano (Origanum sp., Lamiaceae). Mycorrhiza, 16(6), 443-446.
Khoramdel, S., Koocheki, A., Nassiri Mahalati, M., & Ghorbani, R. (2008). Application effects of biofertilizers on the growth indices of black cumin (Nigella sativa L.). Iranian Journal of Crop Research, 6(2), 285-294. DOI: 10.22067/GSC.V6I2.2435. (In Persian).
Kozlowska, J., Stachowiak, N., & Prus, W. (2019). Stability studies of collagen-based microspheres with Calendula officinalis flower extract. Polymer Degradation and Stability, 163, 214-219. DOI: 10.1016/j.polymdegradstab.2019.03.015.
Kumar, V., Sarma, M. K., Saharan, K., Srivastava, R., Kumar, L., Sahai, V., Bisaria, V.S., & Sharma, A. K. (2012). Effect of formulated root endophytic fungus Piriformospora indica and plant growth promoting rhizobacteria fluorescent pseudomonads R 62 and R 81 on Vigna mungo. World Journal of Microbiology and Biotechnology, 28, 595-603. DOI: 10.1007/s11274-011-0852-x
Lehnert, H. A., Serfling, M., Enders, W., & Friedt, F. (2017). Genetics of mycorrhizal symbiosis in winter wheat (Triticum aestivum). New Phytologist, 779-791. DOI: 10.1111/nph.14595
Miller, A.J., Fan, X., Shen, Q., & Smith, S.J. (2007). Amino acids and nitrate as signals for the regulation of nitrogen acquisition. Journal of Experimental Botany, 59, 111-119. DOI: 10.1093/jxb/erm208.
Mirzaei, M. M., Ghorbani, S., Roozbehani, A., & Sadeghi Shoaa, M. (2016). Investigation of different methods of increasing soil fertility through organic, chemical and biological fertilizers on the essential oil of marigold (Calendula officinalis L). Journal of Agriculture and Plant Breeding, 11(4), 37-53. (In Persian).
Mishra, A.K., Mishra, A., & Chattopadhyay, P. (2018). Screening of acute and sub-chronic dermal toxicity of Calendula officinalis L. essential oil. Regulatory Toxicology and Pharmacology. 98, 184-189. DOI: 10.1016/j.yrtph.2018.07.027.
Moghadasan, Sh., Safipour Afshar, A., & Saeid Nematpour, F. (2016). The Role of Mycorrhiza in Drought Tolerance of Marigold (Calendula officinalis L.). Journal of Crop Ecophysiology, 9(4), 521-532. 
Moradi Marjaneh, E., Golavi, M., Ramroudi, M., & Salouki, M. (2018). Investigation of some qualitative characteristics of rosemary plant under the influence of foliar application of various nutrients at different harvest times. Journal of Crop Production, 11(4), 119-134. DOI: 10.22069/ejcp.2019.13979.2064.
Motahhari, M., Hani, A., & Moradi, P. (2011). The effect of phosphorus fertilizer and mycorrhizal fungus on yield, yield components and active ingredients of marigold. The first national conference on new topics in agriculture. Article COI Code: NCNCA01_273. https://civilica.com/doc/162880/. (In Persian).
Omer, E. A., Said-Al Ahl, H. A. H., El Gendy, A. G., Shaban, Kh. A., & Hussein, M.S. (2013). Effect of Amino Acids Application on Production, Volatile Oil and Chemical Composition of Chamomile Cultivated in Saline Soil at Sinai. Journal of Applied Sciences Research, 9(4), 3006-3021, 2013
Omidbeigi, R. (2000). Production and processing of medicinal plants. Volume I, Second Edition, Tarahan Publication. pp 424. (In Persian).
Oosterhuis, D. M., & Weir, B. L. (2010). Foliar fertilization of cotton. Physiology of Cotton. DOI 10.1007/978-90-481-3195-2-25, p: 272-288. https://www.researchgate.net/publication/226243985_Foliar Fertilization_of_Cotton.
Raeisi, M., Farahani, L., & Palashi, M. (2014). Changes of qualitative and quantitative properties of radish (Raphanus sativus L.) under foliar spraying through amino acid. International Journal of Biosciences, 4(1), 463-468. http://dx.doi.org/10.12692/ijb/4.1.463-468.
Rezakhani, A., & Haj Seyed Hadi, M. R. (2017). Effect of manure and foliar application of amino acids on growth characteristics, seed yield and essential oil of coriander (Coriandrum sativum L.). Iranian Journal of Field Crop Science, 48(3), 777-786. DOI: 10.22059/ijfcs.2017.210745.654144. (In Persian).
Rouphael, Y., & Colla, G. (2018). Synergistic Biostimulatory Action: Designing the Next Generation of Plant Biostimulants for Sustainable Agriculture. Frontiers in Plant Science, 9, 1655. DOI: 10.3389/fpls.2018.01655.
Sabbagh, S.K., Poorabdollah, A., Sirousmehr, A., & Gholamalizadeh-Ahangar, A. (2017). Bio-fertilizers and Systemic Acquired Resistance in Fusarium Infected Wheat. Journal of Agricultural Science and Technology, 19(2), 453-464.
Sanikhani, M., Akbari, A., & Kheiry, A. (2019). Effect of phenylalanine and tryptophan on morphological and physiological characteristics in colocynth plant (Citrullus colocynthis L.). Plant Process and Function, 9(35),317-327.(In Persian).
Sasanelli, N., Anton, A., Takacs, T., Addabbo, T. D., Biro, I., & Malov, X. (2009). Influence of arbuscular mycorrhizal fungi on the nematicidal properties of leaf extracts of Thymus vulgaris L. Helminthologia, 46(4), 230-240. DOI: 10.2478/s11687-009-0043-6.
Shao, Y. D., Zhang, D. J., Hu, X. C., Wu, Q. S., Jiang, C. J., Xia, T. J., Gao, X. B., & Kuca, K. (2018). Mycorrhiza-induced changes in root growth and nutrient absorption of tea plants. Plant, Soil and En‌vironment, 64(6), 283-289. DOI.org/10.17221/126/2018-PSE.
Shafeek, M.R., Hafez, M.M., Mahmoud, A.R., & Ali, A.H. (2014). Comparative Effect on N-Fixing Bacterial with Foliar Application of Amino Acid Mixed on Growth and Yield of Pea Plants (Pisum sativum L.). Middle East Journal of Applied Sciences, 4, 755-761.
Shehata, S. M., Abdel-Azem, H. S., Abou El-Yazied, A., & El-Gizawy, A. M. (2011). Effect of Foliar Spraying with Amino Acids and Seaweed Extract on Growth Chemical Constitutes, Yield and its Quality of Celeriac Plant. European Journal of Scientific Research, 58(2), 257-65.
Mohammadi, S., Tabrizi, L., Delshad, M., & Moteshare Zadeh, B. (2013). Investigation of growth and yield of pot marigold (Calendula officinalis L.) under arbuscular mycorrhizal fungi symbiosis and heavy metal stress conditions. Journal of Ecological Agriculture, 3(2), 48-59. (In Persian).
Slavov, A., Ognyanov, M., & Vasileva, I. (2020). Pectic polysaccharides extracted from pot marigold (Calendula officinalis) industrial waste. Food Hydrocolloids. 101, 105545. DOI: 10.1016/j.foodhyd.2019.105545.
Smith, S. E., & Read, D. J. (2010). Mycorrhizal Symbiosis. Academic Press: Cambridge, MA, USA, ISBN, 978-0-08-055934-6.
Souri, M. K., & Yarahmadi, B. (2015). Effect of Amino Chelates Foliar Application on Growth and Development of Marigold (Calendula officinalis) plant. Plant Production Technology, 15(2), 109-119. (In Persian).
Tantawy, A.S., Abdel-Mawgoud, A.M.R., El-Nemr, M.A., & Ghorra Chamoun, Y. (2009). Alleviation of Salinity Effects on Tomato Plants by Application of Amino Acids and Growth Regulators. European Journal of Scientific Research, 30(3), 484-494.
Torabahmadi, S., Abedy, B., & Saberali, S. F. (2019). Evaluation of some Quantitative and Qualitative Characteristics of Pistachio Plant in Response to Amino Acid Compounds and Seaweed Extract. Journal of Agricultural Knowledge and Sustainable Production, 29(4), 189-204. (In Persian).
Wahba, H. E., Motawe, H. M., & Ibrahim, A. Y. (2015). Growth and chemical compositionof Urtica pilulifera L. plant as influenced by foliar application of some aminoacids. Journal of Materials and Environmental Science, 6 (2), 499-509.
Yaghoubi Suraki, F., Souri, M. K., & Arzani, K. (2018). The effect of foliar application of ammonium sulfate on vegetative growth, yield and characteristics quality of greenhouse cucumber. Science and Technology of Greenhouse Crops, 9(1), 41-50. (In Persian).
Zhang, F., Wang, P., Zou, Y. N., Wu, Q. S., & Kuca, K. (2019). Effects of mycorrhizal fungi on root-hair growth and hormone levels of taproot and lateral roots in trifoliate orange under drought stress. Archives of Agronomy and Soil Science, 65, 1316-1330. DOI: 10.1080/03650340.2018.1563780.