Document Type : Research Paper

Authors

1 Department of Agronomy, Faculty of Agriculture, University of Zabol, Zabol, Iran. E-mail: Kaikha_mahlagha@uoz.ac.ir

2 Corresponding Author, Department of Agronomy, Faculty of Agriculture, University of Zabol. Zabol, Iran. E-mail: dr.dahmardeh@uoz.ac.ir

3 Department of Agronomy, Faculty of Agriculture, University of Zabol, Zabol, Iran. E-mail: ikhamari@uoz.ac.ir

4 Department of chemistry, Faculty of Science, University of Zabol, Zabol, Iran. E-mail: soori@uoz.ac.ir

Abstract

In order to evaluation of the spraying time and cobult nanoparticle fertilizer using chemical and green chemistry methods on morphophysiological indices of cowpea, this research was conducted in a factorial experiment in a the experiment was conducted Randomized Complete Block Design (RCBD) with three replications in Agricultural Research Institute University of Zabol in 2017.The experimental included 4 levels of treatments: (Cobult  nanoparticles of plant origin, chemical origin, plant and chemical composition and control), as the first factor and time of spraying in 2 levels (Step 4 leaf and Step 6 leaf as the second factor). The plant density of cowpea was 20 plants/m2 and irrigation was performed twice a week. The results of analysis of variance showed that the time of foliar application and application of nano particles of chemical and plant origin had a significant effect on all studied traits.  Based on the results of average comparison, the highest amount of chlorophyll a (13.25 μg/g leaf), chlorophyll b (7.50 μg/g leaf), carotenoids (3.63 μg/g leaf), as well as plant height (34.84 %), number of pods per plant (28.26%), biological yield (45.64%), economic yield (59.10) compared to control treatment, iron (0.26 ppm) and leaf protein ( (46/14 ppm) was obtained from the six-leaf stage and from the combined application of cobalt nanoparticles with chemical and plant origin. In terms of all the measured traits, it seems that the simultaneous application of cobalt nanoparticles chemically and vegetative, through improving the photosynthetic system, increasing leaf duration and photosynthesis, will lead to increased biological and economic yield.

Keywords

Arnon, A. N. (1976). Method of extraction of chlorophyll in plants. Agronomy Journal, 23, 121-122.
Ayas, H., & Gulser, F. (2005). The effects of sulfur and humic acid on yield components and macronutrient contents of spinach (Spinacia Oleracea Var. Spinoza). Journal of Biological Sciences, 5(6), 801-804.
Bakkaus, E. B., Gouget, B., Gallien, J. P., Khodja, H., Carrot, F., Morel, J. L., & Collins, R. (2005). Concentration and distribution of cobalt in higher plants: the use of micro-pixe spectroscopy. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 231(1-4), 350-356.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2), 248-254. 
DalCorso, G., Manara, A., Piasentin, S., & Furini, A. (2014). Nutrient metal elements in plants. Metallomics, 6(10), 1770-1788.
Ehlers, J. D., & Hall, A. E. (1997). Cowpea (Vigna unguiculata L. Walp.). Field Crops Research, 53(1-3), 187-204.
Gad, N. (2006). Increasing the efficiency of nitrogen fertilization through cobalt application to pea plant. Research Journal of Agriculture and Biological Sciences, 2(6), 433-442.
Ganj Abadi, F., & Jalili, F. (2014). Effect of nitrogen sources and solubilization of micronutrients on agronomic characteristics and yield of cowpea. Research in Crop Sciences, 7(35), 39-54. (In Persian).
Gheyrati Arani, L., Sharifi, T., & Sheykhi, M. R. (2013). Effect of micronutrient application at different growth stages on growth and yield of common bean (cv. Sanrey). Agroecology Journal, 9(3), 35-47. (In Persian).
Haghighatnia, G., & Rajaei, M. (2003). Investigating the effect of the amount and method of consumption of trace elements on canola yield. 8th Congress of Soil Sciences, 254-259. (In Persian).
Keikha, Gh. Fanaei, H. R., Polshekan Pahlavan, M. R., &  Akbari Moghadam, A. R. (2005). Investigation of the effects of foliar application of zinc, zinc and iron on quantitative and qualitative yield of canola. 9th Iranian Soil Science Congress. Tehran. 149-153. (In Persian).
Khadem Moghadam Igdelou, N., Golchin, A., & Farhadi, Kh. (2020). The effects of nitrogen, molybdenum, and cobalt application on pod yield and nutrient content of bean leaf (Phaseolus vulgaris L.). Journal of Crops Improvement, 22(2), 217-229. (In Persian).
Koocheki, A., & Banayan Aval, M. M. (2009). Pulse Crops. Mashhad: University Jihad Publications, 236p. (In Persian).
Kordi, S., Marsafari, M., Tahmasebi, Z., Shahkarami, Gh., Gerami, L., Taghizadeh, A. A., & Ghanbari, F. (2016). Effect of Foliar Application of Zinc on yield, grain and straw protein of bean (Phaseolus vulgaris) under water deficit stress in Ilam weather conditions. Applied Field Crops Research, 29(2), 105-114. (In Persian).
Lithourgidis, A. S., Vasilakoglou, I. B., Dhima, K. V., Dordas, C. A., & Yiakoulaki, M. D. (2006). Forage yield and quality of common vetch mixtures with oat and triticale in two seeding ratios. Field crops Research, 99(2-3), 106-113.
Ma, X., Geiser-Lee, J., Deng, Y., & Kolmakov, A. (2010). Interactions between engineered nanoparticles (ENPs) and plants: Phytotoxicity, uptake and accumulation. Science of the Total Environment, 408(16), 3053-3061.
Mahajan, P., Dhoke, S. K., & Khanna, A. S. (2011). Effect of nano-ZnO particle suspension on growth of mung (Vigna radiata) and Gram (Cicer arietinum) seedlings using plant agar method. Journal of Nanotechnology, pp: 1-7.
Mazaherinia, S., Astaraei, A. R., Fotovat, A., & Monshi, A. (2010). Nano-iron-oxide particles efficiency on Fe, Mn, Zn and Cu concentrations in wheat plant. World Applied Sciences Journal, 7(1), 36-40. (In Persian).
Mishra, S., Keswani, C., Abhilash, P. C., Fraceto, L., & Singh, H. B. (2017). Integrated approach of agri-nanotechnology: challenges and future trends. Frontiers in Plant Science, 8, 307-315.
Mortvedt, J. J. (2003). Research techniques with micronutrient fertilizers for use in deficient crop production, National Fertilizer and Environmental Research Center Tennesee USA, 267-275.
Nasrollahzadeh Asl, A., & Gorbannezhad, H. (2014). Effect of biological and mineral phosphorus fertilizers together with microelement sprayings on yield and component of yield in pinto bean (Phaseolus vulgaris L.). Journal of Crop Ecophysiology, 8(4), 451-464. (In Persian).
Purushothaman, K., & Nagarajan, G. (2009). Performance, emission and combustion characteristics of a compression ignition engine operating on neat orange oil. Renewable Energy, 34(1), 242-245.
Rafique, E., Yousra, M., Mahmood-ul-Hassan, M., Sarwar, S., Tabassam, T., & Choudhary, T. K. (2015). Zinc application affects tissue zinc concentration and seed yield of pea (Pisum sativum L.). Journal of Pedosphere, 25(2), 275-281.
Rahman Khan, M., & Mahmud Khan, M. (2010). Effect of varying concentrations of nikel and cobalt on the plant growth and yield of chickpea. Australian Journal of Basic and Applied Sciences, 4(6), 1036-1046.
Ravi, S., Channal, H. T., Hebsur, N. S., Patil, B. N., & Dharmatti, P. R. (2008). Effect of sulphur, zinc and iron nutrition on growth, yield, nutrient uptake and quality of safflower (Carthamus tinctorius L). Karnataka Journal Agriculture Science, 21(3), 382-385.
Roshdi, M., Boyaghchi, D., & Rezadoust, S. (2012). Effect of micronutrients on growth and yield of pinto bean under irrigation– cutback treatments. Journal of Crop production and processing, 2(5), 131-142. (In Persian).
Ross, S. M., King, J. R., O'Donovan, J. T., & Spaner, D. (2005). The productivity of oats and berseem clover intercrops. I. Primary growth characteristics and forage quality at four densities of oats. Grass and forage science, 60(1), 74-86.
Shabani, R., & Armin, M. (2017). The effect of foliar application of urea and humic acid in rain-fed conditions on yield and yield components of chickpea. Crop Science Research in Arid Regions, 1(1), 77-88. (In Persian).
Shamloo, A., & Roozbahani, A. (2015). Effect of amino acids and microelements on the rate of photosynthetic pigments content and yield of red bean (Phaseolus Vulgaris L.). Journal of Plant Ecophysiology, 7(21), 136-150. (In Persian).
Sharafi, M., & Ranjbar, A. (2011). Determining the tolerance and damage threshold of bean pigments to cobalt. The first national congress of modern agricultural sciences and technologies, 19-21. University of Zanjan. (In Persian).
Sharafi, M., Ranjbar, A., & Beigi Harchegani, H. (2012). The comparison of the effect of soil cobalt concentration on chlorophyll a.b and totall chlorophill density in two cultivars of pinto bean khomein and talash. Crop Physiology Journal, 3(12), 55-68. (In Persian). 
Veisi, A., Pasari, B., & Rokhzadi, A. (2019). Investigating the effect of humic acid and micronutrient nano fertilizers on the response of rainfed chickpea (Cicer arietinum L.) in autumn cultivation. Crop Physiology Journal, 10(40), 93-110. (In Persian).
Wilson, J. R. (1983). Effects of water stress on in vitro dry matter digestibility and chemical composition of herbage of tropical pasture species. Australian Journal of Agricultural Research, 34(4), 377-390.
Yamaguchi, T., Tsukada, C., Takahama, K., Hirotomo, T., Tomioka, R., & Takenaka, C. (2019). Localization and speciation of cobalt and nickel in the leaves of the cobalt-hyperaccumulating tree Clethra barbinervis. Trees, 33(2), 521-532.
Ziaeyan, A. H., & Rajaie, M. (2009). Combined effect of zinc and boron on yield and nutrients accumulation in corn. International Journal of Plant Production, 3(3), 35-44. (In Persian).