To evaluate the effect of different application methods of humic acid and aminochelate (Foliar application and soil application) on growth and yield of greenhouse-cucumber (Cucumis sativus. L), an experiment research at greenhouse for two years was carried out with three replications with randomized complete block design with factorial arrangement, with three levels of humic acid (no humic acid, soil humic acid and leaf-spray humic acid) and three levels of aminocelate (without aminochelate application, soil aminoclate application and leaf aminochelate application). The effect of humic acid application and the effect of amino acid application on fruit yield per unit area, number of fruits per plant, fruit dry matter percentage and leaf chlorophyll index were significant. Also, the effect of humic acid application, the effect of amino acid application and the interaction effect of humic acid and amino acid application on the concentration of nutrients consumed and low leaf consumption were significant. The highest mean fruit yield per unit area was obtained from the application of humic acid soil at 28.7 kg / m2, which increased yield by 24.7% compared to the control treatment (without using humic acid and aminoclate). According to the results, to achieve maximum yield per unit area, use of humic acid and aminocelate in soil were recommended.