The use of different nutritional sources is one of the effective ways to maintain soil quality and improve the quantitative and qualitative yield of plants. The aim of this study was to investigate the effect of organic, biological and chemical fertilizers on some quantitative and qualitative traits in Isabgol medicinal plant in 2018-19 crop year in a randomized complete block design with 6 treatments and 4 replications in Iranshahr. Fertilizer treatments were: Vermicompost (10 tons per hectare), Animal manure (20 tons per hectare), Phosphate Barvar 2 biofertilizer (100 g/ha), Phosphate Barvar 3 biofertilizer (100 g/ha), Superphosphate triple (50 Kg/ha) and no fertilizer application (control). The results showed that the effect of fertilizers on most of the studied traits was significant at the 1% level. The effect of treatments on plant Height and Carbohydrates was not significant and on Inflation index and Chlorophyll number were significant at 5% level. Maximum number of tillers per plant, number of spikes, spike length, fresh weight of plant, swelling index, Carbohydrate, Nitrogen, Potassium and Chlorophyll number were obtain under the influence of manure, and traits including root length, plant height, number of seeds per spike, dry weight Plant, 1000-seed weight, plant yield, yield per hectare, mucilage, protein, chlorophyll a, chlorophyll b and total chlorophyll were obtained under the influence of Vermicompost. Also, control treatment and Phosphate Barvar 2 biofertilizer had the highest swelling and the highest percentage of phosphorus, respectively.