To investigate the effects of row spacing and nitrogen application rate on growth and yield of quinoa, a split plot experiment was conducted in a randomized complete block design with three replications in Sabzevar during 2018. The factors studied were row spacing at three levels (25, 50 and 75 cm) and nitrogen application rate at four levels (0, 50, 100, and 150 kg N ha-1). The results showed that the highest content of photosynthetic pigments, panicle length, number of seeds per plant and biological yield were obtained by 150 kg N ha-1 treatment. However, the 100 kg N ha-1 treatment had the highest number of panicles per plant (19.24) and 1000-seed weight (3.51 g). Increasing row spacing increased chlorophyll contents, plant height, number of panicles, panicle length and number of seeds per plant, but decreased 1000-seed weight, seed yield and biological yield. The decrease in seed yield by increasing the row spacing to 50 and 75 cm was 18.62 and 50.14%, respectively. The highest seed yield (6644.5 kg ha-1) was produced with application of 150 kg N ha-1 at a row spacing of 25 cm. With increasing row spacing, nitrogen requirement to produce maximum seed yield decreased, so that the highest seed yield in 75, 50 and 25 cm rows spacing were produced with the application of 50, 100 and 150 kg N ha-1, respectively. According to the results, the application of 150 kg N ha-1 and row spacing of 25 cm is recommended for planting quinoa, Sajama genotype.