Mycorrhiza is a common group of microorganisms that can establish symbiotic relationships with plants. To evaluate symbiosis and compatibility between a collection of chicory seeds and mycorrhizal fungi, through inoculation, a factorial experiment was set up in a randomized complete block design. Two batches of chicory seed (Ardabil and Fars) were inoculated by two species of mycorrhizal fungi (Glomus mosseae and Glomus intraradices). The control group of seeds were not inoculated. Three replicates were arranged in a greenhouse. The research was aimed at examining how the two species of mycorrhizal fungi affect several plant traits and the amounts of phytochemical compounds (i.e. phenol, flavonoids, antioxidant activity, chlorophyll a and b, total chlorophyll), fresh and dry weights of roots and root length, as well as the ability of chicory roots to absorb several elements. The results showed that seed batch and mycorrhizal fungi have significant effects on vegetative traits, chlorophyll content, phenol, total flavonoids and antioxidant activity in plants. The highest amounts of total phenol production (3.77 mg/g fresh weight), total flavonoids (1.21 mg/g fresh weight), antioxidant activity (5.23 mg/g fresh weight), root growth and chlorophyll content were observed in the Ardabil batch when treated with G. intraradices. The two batches of seeds grew into plants with different growth rates and phytochemical compositions. The growth parameters of plants improved in the case of mycorrhizal inoculation. The effects of G. intraradices were more efficient than those of G. mosseae in symbiosis with chicory plants.