In this study, the effect of combined application of biochar, Azospirillium lipofrom bacteria and nitrogen in two flooding and intermittent irrigation methods on yield and yield components of rice (Oryza sativa L. cv. ‘Tarom Hashemi’) was investigated. This experiment was done in split-split plot arrangement based a complete randomized block design with three replications at the research farm of Sari Agricultural Sciences and Natural Resources University in the two consecutive years of 2017 and 2018. The treatments were two irrigation methods (flooding and alternation irrigation regimes) as main plot and nine fertilizers levels (100, 75 and 50% nitrogen in combination with control (without biochar), 20 and 10 tons of biochar per hectare) and seedling inoculation with Azospirillum lipoferum (inoculation and non-inoculated control) considered as sub and sub sub plots, respectively. Accordings to the significance of the studied factors effects on the measured traits, application of 20 tons of biochar along with 75 to 100% of the recommended nitrogen showed the greatest impact on rice yield and yield components in both intermittent and flooding irrigation methods. Since, the effectiveness of biochar in imtermittent irrigation was more significant; therefore, it can be concluded that the application of 20 tons of biochar per hectare plus 75 to 100% of the recommended nitrogen could greatly compensate the yield reduction due to reduced water consumption in intermittent irrigation as compared to the flooding irrigation.