Document Type : Research Paper


1 Ph.D. Student, Department of Agronomy, Takestan Branch, Islamic Azad University, Takestan, Iran.

2 Associate Professor, Department of Agronomy, Takestan Branch, Islamic Azad University, Takestan, Iran.

3 Assistant Professor, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran.

4 Assistant Professor, Department of Agronomy, Takestan Branch, Islamic Azad University, Takestan, Iran.


The current study tries to evaluate the effect of auxin foliar application in two safflower cultivars under drought stress condition, using a factorial split plot experiment with randomized complete block design in two years (2017-2018 and 2018-2019). It has been conducted at research field of Seed and Plant Improvement Research Institute in Karaj and evaluates irrigation at two levels including normal irrigation (control) and interruption of irrigation from the beginning of grain filling stage (drought) in the main plots and cultivars of Padideh and Goldasht and auxin foliar application in two levels of non-foliar application of distilled water (control) and foliar application of auxin (indole acetic acid) in one step (mid-flowering) at a concentration of 3000 mg.l-1 (30 ppm) in sub-plots as a factorial. In the auxin foliar application and non-foliar application (control) in both normal irrigation and drought stress treatments, the highest total number of heads per plant in Goldasht cultivar have been 26.3 and 24.3 respectively, and the highest grain number per plant observed in Goldasht cultivar have been at 708 and 592, respectively. Auxin foliar application and irrigation has had no significant effect on the grain oil percentage. Goldasht cultivar demonstrates a higher grain yield compared to Padideh cultivar due to lower head temperature, higher soluble carbohydrate concentration, number of heads, and 1000-grain weight. Generally, results show that the effect of the studied treatments is affected by the year and despite the positive effect of auxin foliar application on some yield components at both irrigation levels, foliar application of auxin at middle of flowering stage has no significant effect on grain yield.


Abeysingha, G.L.D.N. (2015). The effect of auxins on seed yield parameters in wheat, pea and canola grown under controlled environment and western Canadian field conditions. A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Plant Science. Department of Agricultural, Food and Nutritional Science University of Alberta. 148 p.
Achhale, D. (2016). Screening of safflower (Carthamus tinctorius L.) genotypes for drought tolerance. M.Sc.(Ag.) Thesis, Rajmata Vijayaraje Scindia Krishi Vishwavidyalaya, Madhya pradesh.
Asemanrafat, M., & Honar, T. (2017). Effect of water stress and plant density on canopy temperature, yield components and protein concentration of red bean (Phaseolus vulgaris L. cv. Akhtar). International Journal of Plant Production, 11(2), 241-258.
Behdani, M. A., & Mousavifar, B. E. (2011). Effect of insufficient irrigation on plant dry mater and remobilization in three spring safflower genotypes (Carthamus tinctorius L.). Journal of Agroecology, 3(3), 406.
Cakir, R. (2004). Effect of water stress at different development stages on vegetative and reproductive growth of Corn. Field Crops Research, 89, 1-16.
Duan, B., Lu, Y. Yin, C. Junttila, C. & Li, C. (2005). Physiological responses to drought and shade in two contrasting Picea asperata populations. Physiologia Plantarum, 124, 476-484.
Eslam, B. P. (2017). Effect of planting date on reducing growth period of spring safflower cultivars in Tabriz cold and semi-arid climate. Iranian Journal of Field Crops Research, 15(4), 851-860.
Flemmer, A.C., Franchini, M.C., & Lindström, L.I. (2015). Description of safflower (Carthamus tinctorius) phenological growth stages according to the extended BBCH scale. Annals of Applied Biology 166, 331-339.
Fleta-Soriano, E. & Munné-Bosch, S. (2016). Stress memory and the inevitable effects of drought: a physiological perspective. Frontiers in Plant Science, 7, 1-6.
French, S.R., Abu-Zaitoon, Y., Uddin, M.M., Bennett, K. & Nonhebel, H.M. (2014). Auxin and Cell Wall Invertase Related Signaling during Rice Grain Development. Plants (Basel, Switzerland), 3(1), 95-112.
Hsiao, T.C. (1973). Plant Responses to Water Stress. Annual Review of Plant Physiology, 24 (1), 519-570.
Hussain, M.I., Lyra, D.A., Farooq, M., Nikoloudakis, N. & Khalid, N. (2016). Salt and drought stresses in safflower: a review. Agronomy for Sustainable Development, 36(1), 4.
Koutroubas, S.D., Papakosta, D.K., & Doitsinis, A. (2004). Cultivar and seasonal effects on the contribution of pre-anthesis assimilates to safflower yield. Field Crops Research, 90, 263-274.
Mahrokh, A., Nabipour, M., Roshanfekr, H. A., & Choukan, R. (2019). Response of some grain maize physiological parameters to drought stress and application of auxin and cytokinin hormones. Journal of Environmental Stresses in Crop Sciences, 12(1), 1-15. (In Persian)
Moosavifar, B., Behdani, M., Jami Alahmadi, M., & Hosaini Bojd, M. (2011). Effect of deficit irrigation on growth and yield of spring safflowers (Carthamus tinctorius L.) genotypes in Birjand. Agroecology, 2(4), 627-639. (In Persian)
Ohashi, Y., Nakayama, N., Saneoka, H., & Fujita, K. (2006). Effects of drought stress on photosynthetic gas exchange, chlorophyll fluorescence and stem diameter of soybean plants. Biologia Plantarum, 50, 138-141.
Park, J.S., Hye, J., Kim, Cho, H.S., Jung, H.W., Cha, J.Y., Yun, D.J., Oh, S.W., & Chung, Y.S. (2019). Overexpression of AtYUCCA6 in soybean crop results in reduced ROS production and increased drought tolerance. Plant Biotechnology Reports, 13(2), 161-168.
Pasban Eslam, B. (2008). Evaluation of physiological and agronomical characters of spring genotypes of safflower for drought tolerance. Final Report of Research Project. No. 87.346. AREEO. pp. 13-21.
Prins, A.H., & Verkaar, H.J. (1992). Defoliation: do physiological and morphological responses lead to (over) compensatin? In: Ayres, P.G. (Ed.), Pests and Pathogens. Plant Responses to Foliar Attack. Bios Scientific Publishers. Oxford, UK, pp. 13-21.
Qaderi, M.M., Kurepin, L.V., & Reid, D.M. (2006). Growth and physiological responses of canola (Brassica napus) to three components of global climate change: temperature, carbon dioxide and drought. Journal of Plant Physiology, 128, 710-721.
Rahmani, F., Sayfzadeh, S. Jabbari, H. Valadabadi, S.A., & Hadidi Masouleh, E. (2019). Alleviation of drought stress effects on safflower yield by foliar application of zinc. International Journal of Plant Production, 13, 297-308.
Rastogi, A., Siddiqui, A., Mishra, B.K., Srivastava, M., Pandey, R., Misra, P., Singh, M., Shukla, S. (2013). Effect of auxin and gibberellic acid on growth and yield components of linseed (Linum usitatissimum L.). Crop Breeding and Applied Biotechnology, 13(2), 136-143.
Sanchez, F.J., Manzanares, M., De Andres, E.F., Tenorio, J.L., & Ayerbe, L. (1998). Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress. Field Crops Research, 59, 225-235.
Sen Raychaudhuri, S. (2000). The role of superoxide dismutase in combating oxidative stress in higher plants. Botanical Review, 66, 89-98.
Sharma, E., Sharma, R., Borah, P., Jain, M., & Khurana J.P. (2015). Emerging Roles of Auxin in Abiotic Stress Responses. G.K. Pandey (ed.), Elucidation of Abiotic Stress Signaling in Plants, Springer Science+Business Media New York.
Sheligl, H.Q. (1986). Die verwertung orgngischer souren durch chlorella lincht. Planta Journal, 47-51.
Shi, H., Chen, L., Ye, T., Liu, X., Ding, K., & Chan, Z. (2014). Modulation of auxin content in Arabidopsis confers improved drought stress resistance. Plant Physiology and Biochemistry, 82, 209-217.
Singh, D.P., Chaudhary, B.D., Singh, P., Sharma, H.C., & Karwasra, S.P.S. (1990). Drought tolerance in oilseed brassicas and chickpea. Hisar, India: Directorate of Research, Haryana Agricultural University.
Singh, D.P., Singh, P., Kumar, A., & Sharma, H.C. (1985). Transpiration. Cooling as a screening technique for drought tolerance in oilseed Brassica. Annals of Botany, 56, 815-820.
Wolfe, D.W., Henderson, D.W., Hsiao, T.C., & Alvino, A. (1988). Interactive water and nitrogen effects on senescence of maize. I. Leaf area duration, nitrogen distribution, and yield. Agronomy Journal, 80, 859-864.
Yau, S.K. (2006). Winter versus spring sowing of rain-fed safflower in a semi-arid, high-elevation Mediterranean environment. European Journal of Agronomy, 10, 1-8.